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O H C K 293 HE

High Yield Protein Expression

Ready for high yield protein production ? Experience HYPE-5tm Transfection Kit

The art of delivery systems


T

he effective transfection solution for bioproduction and biopharmaceutical manufacturing.

Transient transfection of suspension-growing cells represents an attractive alternative to costly and time-consuming stable transfection. HYPE-5TM Transfection Kit is dedicated to achieve High Yield Protein Expression in mammalian cells. It allows for straightforward and easy scale-up to larger volumes for production of milligrams of protein per liter of cell culture with simple and cost efficient handling steps.

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Results CHO Cells

1500

Day 3 Day 5 Day 7

1200 900 600 300 0

HYPE-5

LP

FHD

HEK 293 Cells

10000

µg of SEAP per Liter of cell culture

µg of SEAP per Liter of cell culture

Designed to achieve High Yield Protein Expression in suspension-growing cells Day 3 Day 5 Day 7

8000 6000 4000 2000 0

HYPE-5

LP

FHD

HYPE-5TM Transfection Kit achieves higher potein yield than popular commercial transfection reagents in both CHO and HEK 293 cells adapted in suspension. Secreted Alkaline Phosphatase (SEAP) was produced by transient transfection using HYPE‑5TM, LP or FHD transfection reagents according to manufacturer’s instruction. At day 3, 5 and 7, 20µL supernatants were collected and SEAP activity was measured.

CHO Cells

1200 1000 800 600 400 200 0

HYPE-5

FSM reagent

µg of protein per Liter of cell culture

µg of SEAP per Liter of cell culture

Outperforms competitor: Secreted and intracellularly expressed protein HEK 293 Cells

ß-Gal Expression SEAP Expression

15000 12000 9000 6000 3000 0

HYPE-5

FSM reagent

HYPE-5 FSM reagent

HYPE-5TM Transfection Kit outperforms commercial reagent specific for protein production in both HEK293 and CHO cells adapted in suspension. SEAP or β-Galactosidase (β-Gal) were produced by transient transfection using HYPE-5TM Transfection Kit or FSM reagent according to manufacturer’s instructions. CHO & HEK293 cells were cultured in 30 mL in 125 mL shaken flasks.

CHO Cells

1500 1200 900 600 300 0

30 mL

300 mL

µg of SEAP per Liter of cell culture

µg of SEAP per Liter of cell culture

Easy and efficient scale-up 10000

HEK 293 Cells

8000 6000 4000 2000 0

30 mL

300 mL

Protein production in both CHO and HEK293 suspension cells can be easily scaled up by using HYPE-5TM Transfection Kit. SEAP was produced by transient transfection using HYPE-5TM Transfection Kit. CHO & HEK293 cells were cultured in 30 mL or 300 mL of medium in 125 mL or 1000 mL shaken flasks respectively.


Ordering information The HYPE-5TM Transfection Kit includes HYPE-5TM reagent (DNA transfection reagent) and HYPE-Blast (improves gene expression). HYPE-Blast is optional and cell type-dependent. You can also purchase the kit components separately. All components are completely animal origin free.

HYPE-5TM Transfection Kit Cat # HY01500

Description HYPE-5 Starter Kit 1.5mL Suitable for 0.5-1 L of cell culture HYPE-5 Kit 3mL Suitable for 1-2 L of cell culture HYPE-5 Kit 15mL Suitable for 5-10 L of cell culture HYPE-5 Kit 30mL Suitable for 10-20 L of cell culture

HY03000 HY15000 HY30000

Pkg. Size 1x1.5mL (reagent) + 1x5mL (blast) 2x1.5mL + 2x5mL 1x15mL + 1x50mL 2x15mL + 2x50mL

HYPE-5TM reagent Cat # HYR10003 HYR10015 HYR20030

Description HYPE-5 reagent 3mL Suitable for 1-2 L of cell culture HYPE-5 reagent 15mL Suitable for 5-10 L of cell culture HYPE-5 reagent 30mL Suitable for 10-20 L of cell culture

Pkg. Size 2x1.5mL

Description HYPE-Blast 5mL

Pkg. Size 1x5mL

1x15mL 2x15mL

HYPE Blast Cat # HYB00005

OZ Biosciences: The art of delivery systems At OZ Biosciences we are committed to creating transfection reagents that make your life science research easier. As an innovative, technology-based company and your reliable partner, we continually provide enabling technologies for the delivery of molecules. To stay close to your needs, we listen to you. We encourage your suggestions and feedback. Visit us at www.ozbiosciences.com Rely on OZ Biosciences for: - A broad and comprehensive line of specialty products for the delivery of biomolecules - A proven track record of performance evidenced by >500 citations - A dedicated customer service that is going out of its way to provide the best possible options - A 10 years expertise

The art of delivery systems

Parc Scientifique de Luminy - Batiment Grand Luminy - Case 922 - 13288 Marseille Cedex 9 - FRANCE +33 (0) 486 948 516 / +33 (0) 486 948 515 (fax)

www.ozbiosciences.com - contact@ozbiosciences.com


Hype-5 Protein Production