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SILICA GEL COLUMN CHROMATOGRAPHY


SILICA GEL COLUMN CHROMATOGRAPHY Column chromatography is used for separating and purifying the liquids and solid compounds. It is an effective chromatography technique in which stationary phase adsorbs the unwanted compounds and other impurities using silica gel and activated aluminum oxide powder as powerful adsorbents


Benefits of Silica Gel Column Chromatography • Optimize separation efficiency • High adsorption capacity with large surface area • Reproducible performance • Suitable for large scale applications • Excellent flow rates • Direct scalability


General uses of chromatography in our real life are •

• • • • •

Pharmaceutical Company –determine amount of each chemical found in new product. Hospital –detect blood or alcohol levels in a patient’s blood stream. Law Enforcement – to compare a sample found at a crime scene to samples from suspects. Environmental Agency – determine the level of pollutants in the water supply. Manufacturing Plant – to purify a chemical needed to make a product. Biotechnology industry – establishing the purity or concentration of compounds in biotechnological research. Biological application – Chromatography has many applications in biology. It is used to separate and identify amino acids, carbohydrates, fatty acids, and other natural substances. Environmental testing laboratories use chromatography to identify trace quantities of contaminants such as PCBs in waste oil and pesticides such as DDT in groundwater.


Application of Column Chromatography •

• • •

Analytical uses: For analytical purposes, column chromatography finds limited applications. Vestergaard and Sayegh could separate seven urinary steroids within 5 hours which requires 36 hours on a normal column. They have used narrow Teflon tubing packed with aluminum oxide or silica gel. Separation of geometrical isomers: The separation of cis/trans isomer is based on the steric factors. Isomers whose functional groups can approach the surface of the adsorbent more easily are more strongly adsorbed. Separation of Diastereomers Separation of tautomeric mixtures. Separation of racemates.


Types of silica gel for column chromatography mesh size. SILICA GEL 60-120 MESH Related Categories:

Flash Chromatography, TLC

Grade:

Laboratory Grade

Particle Size:

60-120 Mesh (250125μm)

Boiling Point:

2230 0C

Melting Point:

>1600 0C

Bulk Density KG/LIT

500-650

Ph 10% aq solution

7 ± 0.5

Fe (Iron)

<75 ppm

Chloride(NaCl)

Max 300ppm

Pore Diameter –A

40-60A

Surface Area m2/gm

350-450

Moisture Content %

<6.00


SILICA GEL 60-200 MESH Related Categories:

Flash Chromatography, TLC

Grade:

Laboratory Grade

Particle Size:

60-200 Mesh (25074μm)

Boiling Point:

2230 0C

Melting Point:

>1600 0C

Bulk Density KG/LIT

545-620

Ph 10% aq solution

7 ± 0.5

Fe (Iron)

<75 ppm

Chloride(NaCl)

Max 300ppm

Pore Diameter –A

40-60A

Surface Area m2/gm

350-450

Moisture Content %

<5.00


SILICA GEL 70-230 MESH Related Categories:

Flash Chromatography, TLC

Grade:

Laboratory Grade

Particle Size:

70-230 Mesh (21063μm)

Boiling Point:

2230 0C

Melting Point:

>1600 0C

Bulk Density KG/LIT

500-650

Ph 10% aq solution

7 ± 0.5

Fe (Iron)

<75 ppm

Chloride(NaCl)

Max 300ppm

Pore Diameter –A

40-60A

Surface Area m2/gm

350-550

Moisture Content %

<5.00


SILICA GEL 100-200 MESH Related Categories:

Flash Chromatography, TLC

Grade:

Laboratory Grade

Particle Size:

100-200 Mesh (14974μm))

Boiling Point:

2230 0C

Melting Point:

>1600 0C

Bulk Density KG/LIT

500-650

Ph 10% aq solution

7 ± 0.5

Fe (Iron)

<75 ppm

Chloride(NaCl)

Max 300ppm

Pore Diameter –A

40-60A

Surface Area m2/gm

350-550

Moisture Content %

<6.00


SILICA GEL 200-400 MESH Related Categories:

Flash Chromatography, TLC

Grade:

Laboratory Grade

Particle Size:

200-400 Mesh (7437μm)

Boiling Point:

2230 0C

Melting Point:

>1600 0C

Bulk Density KG/LIT

500-650

Ph 10% aq solution

7 ± 0.5

Fe (Iron)

<65 ppm

Chloride(NaCl)

Max 300ppm

Pore Diameter –A

40-60A

Surface Area m2/gm

450-650

Moisture Content %

<5.00


SILICA GEL 230-400 MESH Related Categories:

Flash Chromatography, TLC

Grade:

Laboratory Grade

Particle Size:

230-400 Mesh (6337μm)

Boiling Point:

2230 0C

Melting Point:

>1600 0C

Bulk Density KG/LIT

400-650

Ph 10% aq solution

7 ± 0.5

Fe (Iron)

<60 ppm

Chloride(NaCl)

Max 300ppm

Pore Diameter –A

40-60A

Surface Area m2/gm

450-650

Moisture Content %

<5.00


SILICA GEL 400-800 MESH Related Categories:

Flash Chromatography, TLC

Grade:

Laboratory Grade

Particle Size:

400-800 Mesh (3718μm)

Boiling Point:

2230 0C

Melting Point:

>1600 0C

Bulk Density KG/LIT

400-650

Ph 10% aq solution

7 ± 0.5

Fe (Iron)

<60 ppm

Chloride(NaCl)

Max 300ppm

Pore Diameter –A

55-60A

Surface Area m2/gm

450-650

Moisture Content %

<5.00


Reference Link • http://column-chromatography.com/chromatography-adsorbents/ • http://column-chromatography.com/thin-layer-chromatography-2/ • http://column-chromatography.com/silica-gel-column-chromatography/ • http://column-chromatography.com/silica-gel-100-200-mesh/ • http://column-chromatography.com/silica-gel-230-400-mesh/

• http://column-chromatography.com/aluminium-oxide-neutral/ • http://column-chromatography.com/aluminium-oxide-basic/ • http://column-chromatography.com/flash-chromatography-3/ • http://column-chromatography.com/blog/ • http://column-chromatography.com/silica-gel-gf/ • http://column-chromatography.com/silica-gel-60-120-mesh/ • http://column-chromatography.com/silica-gel-powder/

• http://column-chromatography.com/aluminium-oxide-chromatography/ • http://column-chromatography.com/extraction-of-bio-active-compounds-from-medicinal-plants/


For More Information Contact Us 304-307, III Floor, Prayosha Complex, Next to Hyundai Motor Showroom, Chhani Jakat Naka, Vadodara â&#x20AC;&#x201C; 390024, Gujarat, India. Phone : +91-265-2761041 Toll Free : 1800-233-2677 Website: Email:

http://column-chromatography.com/ sales@sorbeadindia.com


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silica gel for column chromatography mesh size  

Silica Gel Column chromatography is used for separating and purifying the liquids and solid compounds. It is an effective chromatography tec...

silica gel for column chromatography mesh size  

Silica Gel Column chromatography is used for separating and purifying the liquids and solid compounds. It is an effective chromatography tec...

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