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COLI 3000 Water Quality

BACTERIOLOGICAL CONTROL OF WATER Bacteriological analysis of water is an




absolute requisite to detect pollution and prevent any health hazards. The aim here was clearly to automatize the method used in laboratory for a

IN • drinking and beach water

24/7 detection of Total Coliforms and E.Coli and thus enable the authorities

COLI 3000 benefits :

to take corrective action.

 Quick & reliable pollution alert  Automatic sampling  Continuous measurement  Limited maintenancelimitée

BACKGROUND & APPLICATIONS SERES KNOWHOW in the field of water analysis. Laboratory analysis vs automatic analyser : • Conventional laboratory method : manual sampling during working hours and subsequent laboratory analysis precluding continuous control. • Online sampling and analysis : automatic monitoring providing continuous surveillance of the water microbiological quality.

COLI 3000, online analyser with automatic sampling, a major asset for users : • To ensure a faster and more precise detection of an eventual pollution, • To take quick action if urgent sanitary steps need to be implemented.

APPLICATIONS in the following areas : • Biological analysis of drinking water and surveillance of quality of underground or surface water, mainly for contamination by total coliforms, • Beach water, sea water, waste water (after disinfection), mainly for the presence of E.Coli. Coli 3000_Brochure_UK_120309 - Non contractual document

KEY FEATURES Acknowledged detection method : automatization of the laboratory measurement Selective & precise detection : 1 coliform in 100 ml water) Surveillance of Total Coliforms or E.Coli and other heat resistant coliforms Quick alert in case of pollution peak Response time from sample intake : from 3 to 17 hrs Automatic sampling frequency is programmable from evey 6 hrs up to once a day Reagent is a culture medium allowing optimum development of Coliform bacteria and E.Coli

COLI 3000 Water Quality ANALYTICAL METHOD & BLOCK DIAGRAM The analyser includes 3 measuring chambers and can work in either :

• Automatic, continuous operation : at minimum 6 hourly intervals 100 ml water is sampled from a fast loop by the analyser.

• Semi-automatic operation : manual sampling (three 1-liter containers filled in from one up to 3 different sites to control). Automatic filling of the measuring chambers by the analyser. The complete measuring cycle lasts 18 hrs with the following steps :

- Sampling,

Principle :

- Injection du réactif dans la cuve de me-

 The reagent is a culture medium allowing optimum development of colifoms with a nutriment that is specific to coliforms and E.Coli metabolism (ONPG).

sure régulée en température,

- Incubation and continuous measurement of optical density for a period of 17 hrs or until development of a yellow colouration revealing the presence of coliforms,

- Emptying of the measuring chamber and sterilization of the hydraulic circuit prior to the next emasurement cycle.

 The b-galactosidase enzyme present in coliforms and E.Coli hydrolyses the ONPG and releases an ONP molecule (Orthonitrophenyl) that yields a yellow colouration in the incubation medium.

 The colour change is detected by the analyser. Total coliforms are currently found in higher concentration than E.Coli and as such used as an early indicator of water contamination.



Cabinet Weight Material

Dimensions : 700 x 600 x 400 mm (W x H x D) 35 kg Steel Epoxy finish

User interface

Colour LCD graphic display with control tabs

Environment & Protection

Installation in safe and sheltered area, away from corrosive atmospheres. Cabinet IP65

Output signals & Alarms

Dry contact output relays : Vessel contamination, temperature failure, microprocessor failure, analyser failure


1 RS232 current loop output / Modem on option


230 VAC 50 Hz (110 VAC 60 Hz on request)



300 W


Nutritive medium (reagent) optimized for a quick growth of coliforms and colorimetric measurement

Consumption for auto frequency every 6 H : - Reagent (nutritive medium) : 28 doses / week - Sodium hypochlorite (5 g/l) : 7 l / week - Chlrohydric acid (2N) : 2.5 l / week

Vessel cleaning

Before each measure

Circuit sterilization

Before each measure, after contamination / pollution

Automatic zero

Before each measure



Total coliforms or E.Coli & other thermoresistant forms Control of bacteriological hazard in sea or fresh water

Limit of detection

1 coliform in 100 ml water

Samp. frequency

Every 6 H minimun in automatic mode (ajustable)

Cycle duration

Maximum 18 H

Detection time

- Drinking water : less than 18 H to detect 1 or more coliforms in 100 ml - Beach water : less than 8 H to detect 2000 or more E.Coli in 100 ml sea water - Faster response in case of high pollution peak

SERES environnement 360 rue Louis de Broglie La Duranne - BP 20087 13793 AIX EN PROVENCE Cedex 3 - France

SAMPLE Sampling

In automatic mode, sampling from a fast loop with permanent circulation

Sample flowrate

60 to 200 l / h

Sample volume

100 ml

Incubation temperature

35°C for total coliforms, or 44°C for E.Coli and other thermoresistant forms

Tel : Fax : Email : Internet :

+33 (0)4 4297 3737 +33 (0)4 4297 3030

coli 3000 brouche UK 120309