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àÃ×èͧ : ÍÒ¨ÒÏÁ³ªÑ പÊѧ¡ÃÒ¹¹· ¤³ÐÇÔ·ÂÒÈÒʵÏáÅÐà·¤â¹âÅÂÕ ÁËÒÇÔ·ÂÒÅÑÂÃÒªÀѯÊǹ´ØÊÔµ monchaibiot@hotmail.com

ä¿â¤ä«ÂÒ¹Ô¹ (Phycocyanin) : ÊÒÃÊÕ¨Ò¡Êä»ÃÙÅÔ¹‹Ò (6)

¡ÒÃÊÃŒ Ò §ªÕ Ç ÁÇÅ (biomass) áÅÐ«Õ - ä¿â¤ä«ÂÒ¹Ô ¹ (C-Phycocyanin; C-PC) ¢Í§Êä»ÃÙÅÔ¹‹Ò (Spirulina sp.) ¢Öé¹ÍÂÙ‹ ¡Ñº»˜¨¨ÑÂËÅÒ»ÃСÒà ÍÒ·Ô ÊÒ¾ѹ¸Ø¢Í§ÊÒËËÒ ÍسËÀÙÁÔ ¤ÇÒÁ ࢌÁáʧ ¤Ø³ÀÒ¾áʧ ÃÐÂÐàÇÅÒ㹡ÒÃãËŒáʧ ¤‹Ò¤ÇÒÁ໚¹¡Ã´-´‹Ò§ ¤ÇÒÁࢌÁ¢Œ¹¢Í§à¡Å×Í ÍѵÃÒ¡ÒÃàµÔÁÍÒ¡ÒÈ ÍѵÃÒ¡ÒÃàµÔÁ¤ÒϺ͹ä´ÍÍ¡ä«´ ¤ÇÒÁࢌÁ¢Œ¹¢Í§ä¹âµÃਹ ÍÒÂآͧ¡ÅŒÒàª×éÍ »ÃÔÁÒ³ ¡ÅŒÒàª×éÍ áÅСÒáǹ¼ÊÁ ໚¹µŒ¹ «Ö觾ºÇ‹Ò»˜¨¨Ñ·ÕèÊÓ¤ÑÞ·ÕèÊØ´µ‹Í¡ÒÃÊÐÊÁ«Õ-ä¿â¤ä«ÂÒ¹Ô¹ ¤×Í ¤ÇÒÁࢌÁáʧ áÅФÇÒÁࢌÁ¢Œ¹¢Í§ ä¹âµÃਹ ¨Ò¡ÃÒ§ҹÇԨѾºÇ‹Ò¡ÒÃà¾ÒÐàÅÕé§ S. platensis ã¹ÍÒËÒà à¾ÒÐàÅÕé§ÊÒËËÒ modified Zarrouk’s medium (no addition of TiSO4) ·ÕèÍسËÀÙÁÔ 30 ͧÈÒà«Åà«ÕÂÊ áÅÐÁÕ¡ÒáǹµÅÍ´àÇÅÒ ÀÒÂ㵌 ÊÀÒÇСÒÃãËŒáʧᵡµ‹Ò§¡Ñ¹ 4 ÊÀÒÇÐ ¤×Í 1) à¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁ áʧ 50 (low light) äÁâ¤ÃäÍÊäµ¹/µÒÃÒ§àÁµÃ/ÇÔ¹Ò·Õ 2) à¾ÒÐàÅÕé§ÀÒÂ㵌 ¤ÇÒÁࢌÁáʧ 500 (high light) äÁâ¤ÃäÍÊäµ¹/µÒÃÒ§àÁµÃ/ÇÔ¹Ò·Õ 3) à¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧµèÓáÅŒÇà»ÅÕè¹ä»ãËŒ¤ÇÒÁࢌÁáʧÊÙ§·Ñ¹·Õ áÅÐ 4) à¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧÊÙ§áÅŒÇà»ÅÕè¹ä»ãËŒ¤ÇÒÁࢌÁáʧµèÓ ·Ñ¹·Õ ¾ºÇ‹Ò¡ÒÃà¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧµèÓ (LL; 50 äÁâ¤ÃäÍÊäµ¹/ µÒÃÒ§àÁµÃ/ÇÔ¹Ò·Õ) ÊÒËËÒ¨ÐÁÕÊÕà¢ÕÂÇá¡Á¹éÓà§Ô¹ ã¹¢³Ð·Õè¡ÒÃà¾ÒÐàÅÕé§ ÀÒÂ㵌 ¤ ÇÒÁࢌ Á áʧÊÙ § (HL; 500 äÁâ¤ÃäÍÊäµ¹ / µÒÃÒ§àÁµÃ/ÇÔ¹ Ò·Õ) ÊÒËËÒ¨ÐÁÕÊÕàËÅ×ͧ ´Ñ§ÀÒ¾·Õè 1 (a) áÊ´§ãˌNjҤÇÒÁࢌÁáʧÁÕÍÔ·¸Ô¾Åµ‹Í ¡ÒÃÊÐÊÁç¤Çѵ¶Øµ‹Ò§æ ¢Í§Êä»ÃÙÅÔ¹‹Ò ¡ÒÃà»ÅÕè¹á»Å§´Ñ§¡Å‹ÒÇàËç¹ä´ŒªÑ´à¨¹ã¹¡Ò÷´Åͧ·Õèà»ÅÕ蹨ҡ ¡ÒÃà¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧÊÙ§ «Öè§à«Åŏ¨ÐÁÕÊÕàËÅ×ͧáŌǨÐà»ÅÕèÂ¹ä» à»š¹ÊÕà¢ÕÂÇá¡Á¹éÓà§Ô¹àÁ×èÍà»ÅÕè¹ÁÒà¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧµèÓ ã¹ ¢³Ðà´ÕÂǡѹà«Åŏ¨Ðà»ÅÕ蹨ҡÊÕà¢ÕÂÇá¡Á¹éÓà§Ô¹ä»à»š¹ÊÕàËÅ×ͧàÁ×èÍà¾ÒÐ àÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧµèÓáÅŒÇà»ÅÕè¹ä»à¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧÊÙ§ µÒÁÅӴѺ ¡ÒÃà»ÅÕè¹á»Å§´Ñ§¡Å‹ÒǾºÇ‹Òà¡Ô´¨Ò¡¡ÒÃà»ÅÕè¹á»Å§¢Í§ »ÃÔÁÒ³á¤â÷չÍ´ (Caroteinoid) áÅÐä¿â¤äºÅÔâ«Á (Phycobilisome) ઋ¹à´ÕÂǡѺ·Õ辺ã¹ÊÒËËÒª¹Ô´Í×è¹ ¹Í¡¨Ò¡¹Õ龺NjҡÒÃà¾ÒÐàÅÕé§ÀÒÂ㵌 ¤ÇÒÁࢌÁáʧÊÙ§à«Åŏ¨ÐÁըӹǹ¢Í§ gas vacuoles ÁÒ¡¡Ç‹Ò¡ÒÃà¾ÒÐ àÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧµèÓ ´Ñ§áÊ´§ã¹ÀÒ¾·Õè 1 (b) â´Â¨Ð¾ºÍÂÙ‹ºÃÔàdz Ãͺæ àÂ×èÍËØŒÁà«Åŏ áÊ´§ãËŒàËç¹Ç‹Ò»ÃÔÁÒ³ gas vacuoles ·Õèà¾ÔèÁÁÒ¡¢Öé¹¹Õé à¾×èÍ»ÃÐ⪹ã¹¡Òû‡Í§¡Ñ¹à«Åŏ¨Ò¡áʧÁÒ¡¡Ç‹Ò¡ÒÃÅ͵ÑǢͧà«Åŏ «Öè§ ¨Ðª‹ÇÂÅ´»ÃÔÁÒ³áʧ·ÕèÍÒ¨¨Ðä»·ÓÅÒÂʋǹÃѺáʧ (light harvesting complex) áÅÐ໚¹¼ÅãËŒà¡Ô´ photobleaching á¡‹à«Åŏ䴌 ¹Í¡¨Ò¡¨ÐÁÕÍÔ·¸Ô¾Åµ‹Í¡ÒÃÊÐÊÁ¢Í§Ã§¤Çѵ¶ØáÅŒÇ ¤ÇÒÁࢌÁáʧ ÂѧÁÕ ÍÔ ·¸Ô¾ ŵ‹Í ¡ÒÃà¨ÃÔÞàµÔºâµáÅÐͧ¤»ÃСͺµ‹Ò §æ ÀÒÂã¹à«Åŏ¢Í§ Êä»ÃÙÅÔ¹‹ÒÍÕ¡´ŒÇ (µÒÃÒ§·Õè 1) ¡ÒÃà¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧÊÙ§Êä»ÃÙÅÔ¹‹Ò ¨Ðà¨ÃÔ Þ àµÔ ºâµä´Œ Í Â‹ Ò §ÃÇ´àÃç Ç â´ÂÁÕ à ÇÅÒ·ÇÕ ¤Ù ³ (doubling time) »ÃÐÁÒ³ 21-24 ªÑèÇâÁ§ ã¹¢³Ð·Õè¡ÒÃà¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧµèÓ Êä»ÃÙÅÔ¹‹Ò¨Ðà¨ÃÔÞàµÔºâµä´ŒªŒÒ¡Ç‹Ò â´ÂÁÕàÇÅÒ·ÇÕ¤Ù³»ÃÐÁÒ³ 31-34 ªÑèÇâÁ§

42 : January 2013

áÅоºÇ‹Ò¡ÒÃà¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧÊÙ§Êä»ÃÙÅÔ¹‹Ò¨ÐÁÕ ¡ÒÃÊÐÊÁ¤ÅÍâÿÅŏ àÍ (chlorophyll a) ä¿â¤äºÅÔâ»ÃµÕ¹ â»ÃµÕ¹ÅÐÅÒ (soluble protein) áÅÐâ»ÃµÕ¹·Ñé§ËÁ´ (total protein) äÇŒÀÒÂã¹à«Åŏ¹ŒÍ¡NjÒà«Åŏ·Õèà¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁ ࢌÁáʧµèÓ»ÃÐÁÒ³ 3-3.4 à·‹Ò Í‹ҧäáçµÒÁ ËÒ¡à»ÅÕè¹ ¡ÒÃà¾ÒÐàÅÕé  §¨Ò¡¤ÇÒÁࢌ Á áʧÊÙ §ä»à»š ¹ ¤ÇÒÁࢌ Á áʧµè Ó ¡ÒÃÊÐÊÁç¤Çѵ¶Øµ‹Ò§æ ¡ç¨Ðà¾ÔèÁ¢Öé¹Í‹ҧÃÇ´àÃçÇ à»š¹¼ÅãËŒ »ÃÔÁÒ³â»ÃµÕ¹ÅÐÅÒÂáÅÐâ»ÃµÕ¹·Ñé§ËÁ´·ÕèÇÔà¤ÃÒÐˏ䴌à¾ÔèÁ¢Öé¹ ´ŒÇ 㹢³Ð·Õè¡ÒÃà¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧµèÓáÅŒÇà»ÅÕè¹ ä»à»š¹¤ÇÒÁࢌÁáʧÊÙ§ »ÃÔÁҳç¤Çѵ¶ØÀÒÂã¹à«Åŏ¨ÐŴŧ Í‹ҧÃÇ´àÃçÇ Ê‹Ç¹â»ÃµÕ¹ÅÐÅÒÂáÅÐâ»ÃµÕ¹·Ñé§ËÁ´¨ÐŴŧ ¹Œ Í Â¡Ç‹ Ò ÊÀÒÇСÒÃà¾ÒÐàÅÕé  §·Õè ¡ Å‹ Ò Ç¶Ö § ¡‹ Í ¹Ë¹Œ Ò à¾ÃÒÐ ÊÒËËÒÂä´Œ¹Óä¿â¤äºÅÔâ»ÃµÕ¹ ä»ÊÌҧ໚¹â»ÃµÕ¹·Õè¨Ó໚¹µ‹Í ¡ÒÃà¨ÃÔÞàµÔºâµã¹ÊÀÒÇзÕè¤ÇÒÁࢌÁáʧÊÙ§¹Ñè¹àͧ ¹Í¡¨Ò¡¹Õé ¾ºÇ‹Ò¡ÒÃà¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧÊÙ§Êä»ÃÙÅÔ¹‹Ò¨ÐÁÕ¡Òà ÊÐÊÁä¡Åâ¤à¨¹ (Glycogen) ÁÒ¡¡Ç‹Ò¡ÒÃà¾ÒÐàÅÕé§ÀÒÂ㵌 ¤ÇÒÁࢌÁáʧµèÓ

µÒÃÒ§·Õè 1 ͧ¤»ÃСͺ¢Í§Ã§¤Çѵ¶Ø ÊÒûÃСͺ·Ò§ªÕÇà¤ÁÕ áÅÐàÇÅÒ·ÇÕ¤Ù³¢Í§ S. platensis Cl àÁ×èÍ à¾ÒÐàÅÕé§ÀÒÂ㵌¤ÇÒÁࢌÁáʧÊÙ§ (HL; 500 äÁâ¤ÃäÍÊ䵏¹/µÒÃÒ§àÁµÃ/ÇÔ¹Ò·Õ) áÅФÇÒÁࢌÁáʧµèÓ (LL; 50 äÁâ¤ÃäÍÊäµ¹/µÒÃÒ§àÁµÃ/ÇÔ¹Ò·Õ)

àÍ¡ÊÒÃ͌ҧÍÔ§

Nomsawai et al. 1999. Light regulation of phycobilisome structure and gene expression in Spirulina platensis Cl (Arthrospira sp. PCC 9438). Plant Cell Physiol. 40(12): 1194-1202.

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