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DNA LADDERS

These ready-to-use DNA Size Markers are designed for horizontal gel electrophoresis use. The 100 bp ladder and 1 kb ladder represent the two most popular markers used for DNA fragment size determination while the new Low, High and Broad range markers offer the widest sizing range from 125 bp to 10000 bp. All markers are formulated to run accurately and to provide crisp band patterns. They require no additional preparation and contain Bromophenol Blue for ease of use. All markers are supplied at a concentration of 50µg/500µl (0.1µg/µl). 100 bp Ladder DNA Marker (100-3,000 bp)

The 100 bp DNA Ladder contains 11 discrete DNA fragments ranging in size from 100 bp to 3,000 bp. This marker is ideal for the size determination of PCR products.

bp 3,000

ng/10 µl 108

2,000

147

1,500

116

1,000

77

800 700 600 500 400

62 54 46 150 60

300

56

200

60

100

60

1 Kb Ladder DNA Marker

(300-10,000 bp)

The 1 kb DNA Ladder contains 13 discrete DNA fragments ranging in size from 300 bp to 10,000 bp. This marker is ideal for the size determination of digested DNA.

bp

ng/10 µl

10,000 8,000 6,000 5,000 4,000

80 80 80 80 80

3,000 2,500

120 123

2,000

81

1,500

73

1,000

41

700

57

500

61

300

44

• READy • CRISP

BAND PATTERNS

• INCLUDES BROMOPHENOL BLUE FOR EASE OF USE

• STABLE AT ROOM TEMPERATURE

Broad Range Marker (250-8,000 bp)

The Broad Range marker has 11 discrete fragments: 75 ng/10 µl of 8000 bp, 75 ng/10 µl of 6000 bp, 75 ng/10 µl of 5000 bp, 65 ng/10 µl of 4000 bp, 90 ng/10 µl of 3000 bp, 165 ng/10 µl of 2000 bp, 60 ng/10 µl of 1500 bp, 210 ng/10 µl of 1000 bp, 50 ng/10 µl of 750 bp, 60 ng/10 µl of 500 bp, 75 ng/10 µl of 250 bp

bp

ng/10 µl

8,000 6,000 5,000 4,000 3,000 2,000

75 75 75 65 90 165

1,500

60

1,000 750

210 50

500

60

250

75

High Range Marker

bp

ng/10 µl

10,000 8,000 6,000 5,000 4,000

100 100 100 100 100

3,000

230

2,000

70

(1,000-10,000 bp)

The High Range marker has 8 discrete fragments: 100 ng/10 µl of 10000 bp, 100 ng/10 µl of 8000 bp, 100 ng/10 µl of 6000 bp, 100 ng/10 µl of 5000 bp, 100 ng/10 µl of 4000 bp, 230 ng/10 µl of 3000 bp, 70 ng/10 µl of 2000 bp and 200 ng/10 µl of 1000 bp.

Low Range Marker

bp

ng/10 µl

3,000 2,000 1,500

120 220 65

1,000

235

750

70

500

80

250

105

125

105

(125-3,000 bp)

1,000

The Low Range marker has 9 discrete fragments: 120 ng/10 µl of 3000 bp, 220 ng/10 µl of 2000 bp, 65 ng/10 µl of 1500 bp, 235 ng of 1000 bp, 70 ng/10 µl of 750 bp, 80 ng/10 µl of 500 bp, 105 ng/10 µl of 250 bp, and 105 ng/10 µl of 125 bp.

200

Technical Specifications Product Code Size Range Number of bands Reference bands Package concentration Storage Recommended loading vol. Tracking dyes Source

TO USE

Typical Applications

MDNA-100BPH 100-3000bp 12 500, 1500bp 54µg/500µl vial

MDNA-100BP MDNA-1KB MDNA-50BP MDNA-BR 100-1500bp 250bp-10Kb 50-1500bp 100bp-10Kb 11 13 17 19 500, 1500bp 1Kb, 3Kb 200, 500bp 500bp, 1.5 & 3Kb 50µg/500µl vial 50µg/500µl vial 56µg/500µl vial 86µg/500µl vial 6 months at 25°C, 12 months at 4°C & 24 months at - 20°C 5µl/well 5µl/well 5µl/well 5µl/well 5µl/well Orange G, Xylene Cyanol FF, Bromophenol Blue Proprietary plasmids and PCR fragments phenol-extracted following restriction digestion and dissolved in 10mM Tris-HCl (pH 8.0) and 10mM EDTA

MDNA-HR 250bp-25Kb 14 1Kb, 3Kb 52µg/500µl vial

Size determination of DNA and RNA bands on gels

5µl/well

NE W

Powdered and Liquid Buffers

TBE and TAE are available in both powder and liquid form. Both TBE and TAE are widely used to separate nucleic acids by horizontal gel electrophoresis, and occasionally in vertical polyacrylamide gels. TBE has a higher buffering capacity than TAE, which is used for faster separations of linear double-stranded DNA. Dry TBE is supplied in packs of 10 powder sachets to maintain shelf life. Each buffer sachet may be opened as required and reconstituted in distilled water to make 1 litre of working solution.

Technical Specifications TAE

EACH 50X SOLUTION CONTAINS: TRIS-BASE (2.0M FINAL STOCK CONCENTRATION); GLACIAL ACETIC ACID (1.0M); EDTA, PH 8.0 (0.05M); FOLLOWED BY DISTILLED WATER TO 1L

TBE

EACH 10X SOLUTION / POWDER CONTAINS: TRIS-BASE (0.112M FINAL STOCK CONCENTRATION); BORIC ACID (0.112M); EDTA, PH 8.0 (0.02M); FOLLOWED BY DISTILLED WATER TO 1L

Buffers are also provided as ready-made 50x TAE and 10x TBE solutions in 1 and 5 litre volumes. These are ideal for laboratories running horizontal nucleic acid gels on a daily basis that require off-the-shelf working stock solutions.

ORDERING INFORMATION DNA Ladders MDNA-100BPH 100bp DNA ladder, 100-3000bp, 1x500µl vial MDNA-100BP 100bp DNA ladder, 100-1500bp, 1x500µl vial MDNA-1KB 1Kb DNA ladder, 250bp-10Kb, 1x500µl vial Powdered and Liquid Buffers TBEP Powdered Tris-Borate-EDTA Running Buffer – 10 sachets (1 litre / pack) TBE10X1L Buffer Tris-Borate-EDTA Running Buffer, 10 x 1L TBE10X5 Buffer Tris-Borate-EDTA Running Buffer, 10 x 5L

MDNA-50BP MDNA-BR MDNA-HR

50bp DNA ladder, 50-1500bp, 1x500µl vial Broad Range DNA ladder, 100bp-10Kb, 1x 500µl vial High Range DNA ladder, 250bp-25Kb, 1x500µl vial

TAE50X1L TAE50X5L

Buffer Tris-Acetate-EDTA Running Buffer, 50 x 1L Buffer Tris-Acetate-EDTA Running Buffer, 50 x 5L

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Biocom online cat 16  

Biocom catalogue 2016

Biocom online cat 16  

Biocom catalogue 2016