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New Features in SCORE 5.0

Version 5.x.T/2012


New Features in SCORE 5.0 SCORE 5.0 has been expanded and now features: • • • •

a newly designed short cut version for accelerated work flow full multiplex support of SSP mixes interactive gel visualisation for SSP-mixes of interest instant switch between standard nomenclature, sub group nomenclature without silent mutation differentiation, P-group nomenclature and G-group nomenclature

SCORE 5.0 is based on Access07 and is delivered with a customised installation run time version package that will not interfere with any pre-existing Access versions. Short cut version: The short cut version provides the short cut functionality of the standard version. The workflow tabs are reduced to Main, Patient/sample, Gel, and Analysis. The user can decide at any point to switch between standard and short cut version. In the start Screen there are 4 quick link buttons that lead users to the respective fields or selection options.

Gel screen: For a new sample, the shortcut version will automaticall generate a gel name and open with the selection box to specify the typing kit. If you prefer to assign a user-defined name to a gel, you can do so by selecting this option in the gel configuarion. If there is a need to import an additional typing kit that has not been used before, you can import it from this screen.

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Results can be entered as true multiplex results, thus providing more accurate typings. If an SSP mix of a well is designed to deliver multiple fragment sizes, you are asked to specify which fragment size you have observed. In order to reduce the list of multiple fragment sizes that differ only by some base pairs, the display will show binned fragment size groups. Groups closest to the actual amplification sizes will be displayed in the list. If you are in doubt, you can select the value “any”, which deactivates this feature for this single multiplex result. If you do not whish to enter multiplex results at all, you can deactivate this feature. In this case, the band that is displayed on the virtual gel will always be the shortest fragment size to aid you to differentiate any primer–dimers on the gel.

You can enter multiple bands in one lane by simultaneously pressing the control key. In any case, if there is the possibility to have multiple fragment lengths, the specific band will show up in purple.

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Gels can be visualised in the traditional dark display of the inverse light display. The settings can be selected in the gel configuration screen. Here you also can select the option to enter a user-defined gel name.

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Typing configuration: The typing options can be set via the configuration tab, typing options.

New options provide you additional flexibility. You can choose dynamically which allele database you want ot use for interpretation. • The current allele database you are logged into • The allele database that was used to design the kit you used for typing. If you combine multiple kits, the earliest allele database will be used. • The allele database that is linked to the design of the kit, but not older than one year • Any specific allele database starting from HLADB_2.13.0 You can select the output format of typing results • Standard allele name • Allele name restricted to main and subgroups, silent mutations will not be listed separately • P-names of alleles • G-names of alleles You can choose the source of serological equivalents based on a publication in Tissue Antigens 2009 (R. Holdsworth, C.K. Hurley, S.G.E. Marsh, M. Lau, H.J. Noreen, J.H. Kempenich, M. Setterholm, M.Maiers: The HLA Dictionary 2008: a summary of HLA-A, -B, -C, -DRB1/3/4/5, -DQB1 alleles and their association with serologically defined HLA-A, -B, -C, -DR and -DQ antigens Tissue Antigens (2009) 73:95-170) • WHO assignment • Expert assignment You can choose to enter SSP-results as multiplex results or just as Yes/NO results with the check box Enter and utilise information of multiple amplification sizes.

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Use of Riboon instead of menus: From Office 07 onward, there are ribbons = extended menus in the place of classical menus. These riboons can either be displayed permanently or they can pop up and hide. The default for SCORE is the setting “minimize the ribbon�. You can modify this by right click in the menu bar layer

In the minimize mode, the ribbon will overlap the display if you click on a menu item. In this mode, you will not be able to see or access the top links Main, Patient/Sample, Gel and Analysis.

Just click anywhere on the SCORE program screen, and the ribbon will be minized again If you inactivate the minimize option, the ribbon will be there permanently, thus requiring some space.

Therefore it is recommended to keep the riboon minimized.

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Analysis screen of short cut Version The Analysis screen provides a listing of all loci typed for the current sample

Clicking on any field of a locus will inflate the analysis area and provide detailed information as well as all tools to re-analyse or partially re-analyse a sample.

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If a sample cannot be analysed directly and requires a tolerance to allow the assumption of false positive/negative reactivities, the new information screen allows you to highlight all weligible positions after you click on one of the bombs.

In this image, you see lane 6 highlighted, corresponding to the position that should be negative for the allele combination A*01:01P and A*02:01.

You can edit the gel results directly in this screen, which will lead to an instant re-analysis of the sample as soon as you click on “Done�.

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Single Allele exclusion typing kits: If kits are designed to exclude an allele, e.g. B*27 or B*5701, negative results will not any more trigger an automatc recalculation, but insert the message “This sample is xxx negative” into the comment field.

Distributed by: OLERUP GmbH Address: Loewengasse 47/6, 1030 Vienna, Austria Orders and support: support-at@olerup.com Tel: +43-1-710 15 00. Fax: +43-1-710 15 00 10

2012-01-V1.0

Manufactured by: OLERUP SSP AB Address: Franzengatan 5 SE-112 51 STOCKHOLM, Sweden E-mail: info-ssp@olerup.com Tel: +46 8 717 88 27. Fax: +46 8 717 88 18

OLERUP Inc Address: 901 S. Bolmar St., Suite R West Chester, PA 19382 Orders and support: info.us@olerup.com Tel: 1-877-OLERUP1. Fax: 610-344-7989

www.olerup-ssp.com

www.olerup.com

Olerup SSP® AB is ISO 9001:2008 and ISO 13485:2003 certified. Olerup SSP® AB is IVD certified in accordance with the requirements of the IVD Directive 98/79/EC. Olerup SSP® HLA Typing Kits are FDA cleared and licensed by Health Canada.

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SCORE 5 New Features