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Reg. No. : .................................... Name : .........................................
Third Semester B.Tech. Degree Examination, June 2009 (2003 Scheme) 03 306 : MICROBIOLOGY (B) Time : 3 Hours
Max. Marks : 100 PART A
Answer all questions. Each question carries 4 marks. 1. Write a brief note on the nutritional types of bacteria. 2. What are antibiotics ? Explain the mode of action of penicillin and tetracycline. 3. Differentiate between bright field and dark field microscopy. 4. Differentiate between prokaryotes and eukaryotes. 5. Give a brief account of two economically important algae. 6. Explain Pasteurization. 7. With a neat labelled diagram describe the growth curve of bacteria. 8. Discuss any two methods for the classification of bacteria. 9. What are endospores ? Name any two bacterial genera producing endospores. 10. Describe the chemical composition of bacterial cell wall and point out the differences between gram positive and gram negative cell wall. (4×10=40 Marks) P.T.O.
*3132* PART B
Answer one question from each Module. Module I 11. a) Give an account of phase-contrast microscopy. How it differ from normal light microscopy ?. b) Outline the different methods for the enumeration of microorganisms in a given soil sample. Discuss the merit and demerits. (10×2=20 Marks) OR 12. a) What are the modern trends in microbial taxonomy ? Explain. b) Differentiate between synchronous and asynchronous culture. Explain how synchrony can be attained. (10×2=20 Marks) Module II 13. a) What are the major methods for the genetic transfer in bacteria ? b) Explain glycolysis. Highlight its significance. OR
14. a) Explain TCA cycle and its importance in microbial metabolism. b) Explain Entuer-Deodroff pathway.
Module III 15. a) Describe the methods of determining the sanitary quality of drinking water. b) Describe the role of microorganisms in maintaining soil fertility. (10×2=20 Marks) OR 16. a) What is sterilization ? What are the most commonly used sterilizing agents ? b) Discuss the various methods adopted for the sterilization of milk. (10×2=20 Marks) ____________________
9.What are endospores ? Name any two bacterial genera producing endospores. 4.Differentiate between prokaryotes and eukaryotes. 5.Give a bri...