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Cytotoxicity of mineral trioxide aggregate (MTA) and bone morphogenetic protein 2 (BMP-2) and response of rat pulp to MTA and BMP-2 • • • •

Hyunjung Ko, DDS, PhD , Wonkyung Yang, DDS, PhD , Kyungtae Park, DDS, Miri Kim, DDS, PhD

Affiliations Reprint requests: Miri Kim, Division of Conservative Dentistry, Department of Dentistry, Asan Medical Center, Ulsan University, 388-1, Pungnapdong, Songpa-gu, Seoul, Korea 138-736 o

Received 28 October 2009; received in revised form 18 January 2010; accepted 29 January 2010.

Objective The aim of this study was to assess the cytotoxicity of mineral trioxide aggregate (MTA) and bone morphogenetic protein 2 (BMP-2) and the response of rat pulp tissue to MTA and BMP-2.

Study design For cytotoxicity studies, 1 g MTA was mixed with or without 1 μg of BMP-2 and allowed to set for 1, 24, 48, or 72 hours before addition of samples to 2-mL aliquots of culture medium. The viability of MG-63 cells was determined using the dimethylthiazol-diphenyltetrazolium bromide (MTT) assay. For animal studies, upper first molars from 32 Sprague-Dawley rats were used, the molars were exposed, and 1 g MTA cement was placed in the first molars. In left molars, 1 μg BMP-2 was additionally placed on exposed pulps with MTA. After 2 weeks and 7 weeks, rats were killed and histologic sections assessed by light microscopy.

Results In MTT assay, the viability was higher in the MTA with BMP-2 group than in the MTA-only group up to 24 hours, but was not significantly different thereafter. In animal study, inflammation was higher in the MTA-only group than in the MTA with BMP group, although this difference did not attain statistical significance.

Conclusions The addition of BMP-2 had a beneficial effect in vitro, reducing the initial cytotoxicity of freshly mixed MTA. However, the pulp reaction to a combination of MTA and BMP2 was not significantly better than use of MTA alone.

Cytotoxicity of mineral trioxide aggregate  
Cytotoxicity of mineral trioxide aggregate  

The aim of this study was to assess the cytotoxicity of mineral trioxide aggregate (MTA) and bone morphogenetic protein 2 (BMP-2) and the re...

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