Electrophoresis Is A Great Experiment To Develop And Demonstrate One important part of physics that lends itself well to practical experiments in the lab is electrophoresis. However, educators must still find the time to make the experiment and obtain the mandatory materials to be able to demonstrate this important concept to their students. Here are a few ideas to do this in an efficient manner. The Essentials At first, the word electrophoresis might seem a bit daunting, but there is a resemblance to the word telekinesis which sharp students often realize quickly. This process has remarkable and almost supernatural results, like telekinesis. At this point in the class, the majority of students are aware of the process of electrostatics, where a charged particle will move in the occurrence of a static electrical field. The electrophoretic effect is similar, in that an electrical field will generate a motive force on a particle in a solution. When brought on by its interface with a liquid, the charged condition of the particle is acted upon. When the liquid induces a positive charge on the particle making this a cation, this is cataphoresis. Conversely, in anaphoresis, a negative charge is induced, and the particle is then called an anion. (Anion may be memorized as anode-ion and cation may be remembered as cathode-ion.) The cation will move in the direction of the field, while the anion will move in the opposite direction. The force of the motion is slowed by two mechanisms. Friction is the first, which is created by the particle traveling through the liquid. This is a force proportionate to the velocity and it is analyzed by the laws of fluid dynamics. In the second, the ions in the particle are acted on by the field which may lie some distance away and not the surface of the particle. This force is called electrophoretic retardation. Experiments To Show The Effect The original demonstration of electrophoresis was performed by Ferdinand Frederic Reuss at the Moscow State University in 1807. The motion of clay particles which migrated on use of an electric field was what he discovered. One effective modern experiment includes separating proteins by the electrophoretic process. In relation to their associated charges, the proteins will disperse at a different rate. The process is very easily seen as the four proteins are each a distinct color. This shows the practical application of utilizing an electrostatic force to filter and separate distinct proteins. A set of multi-colored dye molecules is utilized in a second effective experiment. By following the migration rates in the existence of a static electrical field, students can distinguish the molecules by comparing the rates to a table of known values. A subsequent part of the experiment entails determining the mechanism of binding the dye molecules to DNA. This demonstrates the practical
Modern Biology of Indiana, Inc
Electrophoresis Is A Great Experiment To Develop And Demonstrate application of analyzing DNA strands, which is a well-known process among fans of forensic television shows or giant dinosaur movies. Order Online For Materials Obviously, these are effective and interesting experiments. Of course, they also require the effort of a lesson plan, a rubric, and the lab materials. Don't worry; for a very affordable fee, you can get the whole package online. If you are a teacher that wants to effectively teach the sciences, there are several websites devoted to aiding instructors. You simply need to specify your number of students, and you will quickly receive everything you need to teach this important topic in your own lab. Discover more about electrophoresis by using biology experiments out of Modern Biology. Check out Modern Biology by going to their web site which is www.modernbio.com.
Document Tags: electrophoresis lab, electrophoresis gel, agarose electrophoresis http://www.modernbio.com/
Modern Biology of Indiana, Inc
Published on Mar 7, 2014
Discover more about electrophoresis by using biology experiments out of Modern Biology. Check out Modern Biology by going to their web site...