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Introduction

Purpose

Effect of Fixation

Summary


Preparation of tissue for microscopic examination , through a series of namely fixation , clearing, embedding ,cutting & Staining.

Tissue decompose when removed from the body or it is cut off from the body supply – Autolysis or Putrefaction


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A physical or chemical process that stops as soon as possible vital cellular processes, maintaining with minimal alternation the shape, volume spatial and molecular relation between elements. Fixation is the foundation for the subsequent stages in the preparation of the sections.


1.To prevent autolysis , bacterial decomposition & putrefaction. 2. To coagulate the tissue as prevent loss of easily diffusible substances. 3. Indurate tissues to enhance working with glass slides. 4. To facilitate differential staining with dyes & other reagents.


1.

2. 3. 4.

5. 6.

Inhibitions of autolysis & putrefaction Preservation Hardening Solidification of colloid material Optical differentiation Effect on staining


I.

Immersion

II.

Perfusion

III.

Perfusion – immersion

IV.

Heat fixation


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It is clear that fixation is normally carried out as soon as possible to prevent autolysis and to reduce possible infectivity.

Formalin is used for all routine surgical pathology and autopsy tissues when an H & E slides is to be produced.

Basic Principle of IHC Fixation and Staining - Immunostaining  

Tips on various IHC fixation and permeabilization methods. IHC fixation provides the detail about preserves tissue for future analysis by pr...

Basic Principle of IHC Fixation and Staining - Immunostaining  

Tips on various IHC fixation and permeabilization methods. IHC fixation provides the detail about preserves tissue for future analysis by pr...

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