Page 1

Microbes Me_DIARY

P

01/02

site: me 01/02 hand

EXPOSED (A) date: 23/01/11 time: 00:54-01:03

skin face

EXPOSED (P)

inside nose

THRESHOLD

covered back

CONCEALED


Microbes Me_Collection me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

inside, storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

27.7°C

plate no:

plate notes:

autoclaved petri dish with LB + agar medium prepared by lab collection notes:

me 37°C body temperature, outside temperature 8°C (d)/ 5°C (n), inside temperature 20°C - microbes collected after 18h day (shopping on the market (smell of over roasted onions and bbq burgers), preparing experiment, collecting microbes on mm site, cycling, meeting friends, theatre old victorian spa, dining...

01/02

concealed_covered back

01

P01/02_ 23/01/11


site: me 01/02 hand

EXPOSED (A) date: 23/01/11 time: 00:54-01:03


skin face

EXPOSED (P)

inside nose

THRESHOLD


covered back

CONCEALED


Microbes Me_Development Minutes_w me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

inside, storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

27.7°C

plate no:

01/02_w

concealed_covered back

24/01/11

inside, 27.7°C development minutes:

exposed (A) _ 1 white spot 0.25mm diameter

exposed (P) _ no changes

threshold _ area 17.5mm diameter, white spots 1.5mm diameter each, regular spread, no overlapping

concealed _ no changes

special notes: lots of condensate in dish

02

P01/02_w 24/01/11


Microbes Me_Development Minutes _c

me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

outside storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

24/01/11

outside, 8°C (d), 5°C (n); 85% humidity development minutes:

exposed (A) _ no changes

exposed (P) _ no changes

threshold _ no changes

concealed _ no changes

condit. plate no:

01/02_c

concealed_covered back

02

P01/02_c 24/01/11


Microbes Me_Development Minutes_w me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

inside, storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

27.7°C

plate no:

01/02_w

concealed_covered back

25/01/11

inside, 27.7°C development minutes:

exposed (A) _ 3 white spots, separated; 1x 3mm diameter 2dim. darker centre; 1x 1mm diameter 3dim. drop like; 1x 0.25mm drop like

exposed (P) _ 3 white spots 0.25m diameter separated

threshold _ area 18mm x 22mm, white spots 1-1.5mm diameter each in centre 3dim. drop like, separated, surrounding ring white spots, 2-3mm diameter starting to touch each other; 3dim. drop like

concealed _ no changes

special notes: condensate disappeared

03

P01/02_w 25/01/11


Microbes Me_Development Minutes _c me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

outside storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

25/01/11

outside, 8°C (d), 5°C (n); 83% humidity development minutes:

exposed (A) _ blurred yellowish edges around residue disappeared

exposed (P) _ no changes

threshold _ no changes

concealed _ no changes

condit. plate no:

01/02_c

concealed_covered back

03

P01/02_c 25/01/11


Microbes Me_Development Minutes_w me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

inside, storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

27.7°C

plate no:

01/02_w

concealed_covered back

26/01/11

inside, 27.7°C development minutes:

exposed (A) _ 3 spots, separated; large spot 5mm diameter cloudy; greyish white. darker centre with bloomy network, 2dim; 1x 1mm diameter 3dim. drop like; 1x 0.25mm drop like, both yellow

exposed (P) _ 6 yellowish white drops, 0.25-1.5mm, separated, 3dim

threshold _ area 20mm x 28mm, white spots 1.5-2mm diameter each in centre, 3dim. drop like, merging, surrounding ring white spots, 3-4mm diameter, merging; 3dim. drop like; inner centre of spots more white opaque diameter 5/6 of whole spot

concealed _ no changes

special notes: condensate disappeared

04

P01/02_w 26/01/11


Microbes Me_Development Minutes _c me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

outside storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

26/01/11

outside, 5°C (d), 1°C (n); 73% humidity development minutes:

exposed (A) _ no changes

exposed (P) _ no changes

threshold _ no changes

concealed _ no changes

condit. plate no:

01/02_c

concealed_covered back

04

P01/02_c 26/01/11


Microbes Me_Development Minutes_w me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

inside, storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

27.7°C

plate no:

01/02_w

concealed_covered back

27/01/11

inside, 37.5°C; change of location London/Germany, transport temperature unknown ca.7h development minutes:

exposed (A) _ 4 spots, separated; large spot 9mm diameter, greyish white, cloudy outline, darker centre with bloomy network or nerved, finer network in background 2dim, relief, 2dim - 3dim; 1x 2.5mm diameter, yellow-white, 3dim. drop like; 2x 0.25mm drop like, separated, yellow-white

exposed (P) _ 2 white large spots, 3dim drop like, 2.25mm diameter; 2 yellow large spots, 3dim drop like 2mm diameter, 2 small spots, white, 0.1mm diameter, flat 2dim

threshold _ area 22mm x 30mm, white spots 2mm diameter each in centre, 3dim. drop like, merging, surrounding ring white spots, 5mm diameter, closed circle; 3dim. drop like; inner centre of spots more white opaque diameter 5/6 of whole spot

concealed _ no changes

special notes: no condensate

05

P01/02_w 27/01/11


Microbes Me_Development Minutes _c me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

outside storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

condit. plate no:

01/02_c

concealed_covered back

27/01/11

storage in fridge 2°C constant; move London/Germany, transport temperature unknown ca.7h development minutes:

exposed (A) _ no changes

exposed (P) _ no changes

threshold _ no changes

concealed _ no changes

05

P01/02_c 27/01/11


Microbes Me_Development Minutes_w me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

inside, storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

27.7°C

plate no:

01/02_w

concealed_covered back

29/01/11

inside, 37.5°C development minutes:

exposed (A) _ 4 spots, separated; large spot 1.3mm diameter, greyish white, cloudy outline, darker centre with bloomy network or nerved, finer network in background 2dim, relief, 2dim - 3dim; 1x 2.5mm diameter, yellow-white, 3dim. drop like; 2x 0.25mm drop like, separated, yellow-white; one of them surrounded by a carpet of smaller dots 0.1mm diameter whole area 2.5mm

exposed (P) _ 2 white large spots, 3dim drop like, 3-3.5mm diameter, merging; 2 yellow large spots, 3dim drop like 2.5mm diameter, separated, centre higher than outer skirts; 2 small spots, white, 0.1mm diameter, flat 2dim

threshold _ area 25mm x 30mm, white spots 2mm diameter each in centre, 3dim. drop like, merging, surrounding ring white spots, 5mm diameter, closed circle; 3dim. drop like; inner centre of spots more white opaque diameter 5/6 of whole spot, looks like a flower with blossoms

concealed _ no changes

06

P01/02_w 29/01/11


Microbes Me_Development Minutes _c me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

outside storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

29/01/11

storage in fridge 2°C constant development minutes:

exposed (A) _ no changes

exposed (P) _ no changes

threshold _ no changes

concealed _ no changes

condit. plate no:

01/02_c

concealed_covered back

06

P01/02_c 29/01/11


Microbes Me_Development Minutes_w me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

inside, storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

27.7°C

plate no:

01/02_w

concealed_covered back

31/01/11

inside 27.7°C; move Germany/London, transport temperature unknown ca.7h development minutes:

exposed (A) _ 4 spots, separated; large spot 1.3mm diameter, greyish white, cloudy outline, darker centre with bloomy network or nerved, finer network in background 2dim, relief, 2dim - 3dim, frame around network increased in width 1.5-3mm; 1x 2.5mm diameter, yellow-white, 3dim. drop like; 2x 0.25mm drop like, separated, yellow-white; one of them surrounded by a carpet of smaller merged dots 0.1mm diameter whole area 3mm

exposed (P) _ 2 white large spots, 3dim drop like, 3-3.5mm diameter, merging; 2 yellow large spots, 3dim drop like 2.5-3.5mm diameter, separated, centre higher than outer skirts; 2 small spots, white, 0.1mm diameter, flat 2dim

threshold _ area 25mm x 30mm, white spots 2mm diameter each in centre, 3dim. drop like, merging, surrounding ring white spots, 5mm diameter, closed circle; 3dim. drop like; inner centre of spots more white opaque diameter 5/6 of whole spot, looks like a flower with blossoms - not considerable changed

concealed _ no changes

07

P01/02_w 31/01/11


Microbes Me_Development Minutes _c me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

outside storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

condit. plate no:

01/02_c

concealed_covered back

31/01/11

storage outside 8°C (d) 0°C (n); move Germany/London, transport temperature unknown ca.7h development minutes:

exposed (A) _ no changes

exposed (P) _ no changes

threshold _ no changes

concealed _ no changes

07

P01/02_c 31/01/11


Microbes Me_Development Minutes_w me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

inside, storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

27.7°C

plate no:

08

P01/02_w 02/02/11

08

P01/02_c 02/02/11

01/02_w

concealed_covered back

02/02/11

inside 27.7°C development minutes:

exposed (A) _ 4 spots, separated; large spot 1.3mm diameter, greyish white, cloudy outline, darker centre with bloomy network or nerved, finer network in background 2dim, relief, 2dim - 3dim, frame around network increased in width 1.5-3mm; 1x 2.5mm diameter, yellow-white, 3dim. drop like; 2x 0.25mm, centre raising, separated, yellow-white; one of them surrounded by a carpet of smaller merged dots 0.1mm diameter whole area 3mm diameter

exposed (P) _ 2 white large spots, 3dim drop like, 3-4mm diameter, merging; 2 yellow large spots, 3dim drop like 2.5-3.5mm diameter, separated, centre higher than outer skirts; 2 small spots, white, 0.1mm diameter, flat 2dim- not considerable changed

threshold _ area 25mm x 30mm, white spots 2mm diameter each in centre 10x15mm diameter, 3dim. drop like, merging, surrounding ring white spots, 5mm diameter, closed circle; 3dim. drop like; inner centre of spots more white opaque diameter 5/6 of whole spot, looks like a flower with blossoms - not considerable changed

concealed _ no changes

Microbes Me_Development Minutes _c me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

outside storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

02/02/11

storage outside 7°C (d) 5°C (n); development minutes:

exposed (A) _ no changes

exposed (P) _ no changes

threshold _ no changes

concealed _ no changes

condit. plate no:

01/02_c

concealed_covered back


Bacillus subtilis known also as the hay bacillus or grass bacillus, is a Grampositive, catalase-positive bacterium commonly found in soil.[3] A member of the genus Bacillus, B. subtilis is rod-shaped, and has the ability to form a tough, protective endospore, allowing the organism to tolerate extreme environmental conditions. B. subtilis is not a human pathogen. It may contaminate food but rarely causes food poisoning.[5] B. subtilis produces the proteolytic enzyme subtilisin. B. subtilis spores can survive the extreme heat during cooking. B. subtilis is responsible for causing ropiness — a sticky, stringy consistency caused by bacterial production of long-chain polysaccharides — in spoiled bread dough. Reproduction: B. subtilis can divide symmetrically to make two daughter cells (binary fission), or asymmetrically, producing a single endospore that is resistant to environmental factors such as heat, acid, and salt, and which can persist in the environment for long periods of time. The endospore is formed at times of nutritional stress, allowing the organism to persist in the environment until conditions become favorable. Prior to the process to produce the spore the bacterium might become motile, through the production of flagella, and also take up DNA from the environment.

circular form

raised elevation

entire margin


irregular form Mycobacterium smegmatis lives in aggregate layers of cells attached to each other in flat , wrinkled a community called a biofilm. Mycobacterium smegmatis are mostly found in the soil, water, and plants. They tend mostly to exist near large bodies of undulate margin water. It isn’t dependent on living in an animal, unlike some pathogenic Mycobacterium. The bacteria will be finely wrinkled and creamy white while it is growing on accessible nutrients. When Mycobacterium smegmatis has been growing for quite some time (generally after 48 hr growth) and is abundant, the color will turn from white to a nonpigmented creamy yellow. It will also be waxy because of the high amount of unique Gram-positive cell wall coated with mycolic acids. The bacteria also ranges in textures, being seen as smooth, flat and glistening or coarsely folded or finely wrinkled . Since Mycobacteria are obligate aerobes, oxygen is required for aerobic respiration.


Microbes Me_Development Minutes_w me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

inside, storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

27.7°C

plate no:

09

P01/02_w 05/02/11

09

P01/02_c 05/02/11

01/02_w

concealed_covered back

05/02/11

inside 33°C development minutes:

exposed (A) _ not considerable changed

exposed (P) _ not considerable changed

threshold _ smaller spots at the top of the frame colonies, opaque, white, 3dim, 1mm diameter

concealed _ no changes

special note: condensate in dish

Microbes Me_Development Minutes _c me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

outside storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

05/02/11

storage outside 12°C (d) 10°C (n); development minutes:

exposed (A) _ no changes

exposed (P) _ no changes

threshold _ no changes

concealed _ no changes

special note: wide spread white spots, 3 dim, 0.75mm diameter

condit. plate no:

01/02_c

concealed_covered back


Microbes Me_Development Minutes_w me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

inside, storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

27.7°C

plate no:

10

P01/02_w 07/02/11

10

P01/02_c 07/02/11

01/02_w

concealed_covered back

07/02/11

inside 33°C development minutes:

exposed (A) _ no changes

exposed (P) _ no changes

threshold _ smaller colonies at the top of the frame colonies, opaque, dark white, raised elevation, 1mm diameter, circular form, entire margin, opaque

concealed _ no changes

special note: condensate in dish has reduced

Microbes Me_Development Minutes _c me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

outside storage:

condit. plate no:

01/02_c

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; concealed_covered back locations:

storage notes:

date:

07/02/11

storage outside 11°C (d) 3°C (n); development minutes:

exposed (A) _ no changes

exposed (P) _ no changes

threshold _ no changes

concealed _ no changes

special note: scattered 17 white colonies, spread area 40x40mm, raised elevation, 2mm diameter, circular form, entire margin, smooth surface, opaque 1 yellow colony, separated, raised elevation, smooth surface, opaque

0.75mm diameter, circular form, entire margin,


Microbes Me_Development Minutes_w me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

inside, storage:

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; locations:

storage notes:

date:

27.7°C

plate no:

11

P01/02_w 09/02/11

11

P01/02_c 09/02/11

01/02_w

concealed_covered back

09/02/11

inside 33°C development minutes:

exposed (A) _ no further growth of previous colonies but surrounding flat colonies, clear frame of 2mm around older edges.

exposed (P) _ surrounded by flat colonies

threshold _ smaller colonies at the top of the frame colonies, opaque, greyish white, raised elevation, 1mm diameter, circular form, entire margin, opaque

concealed _ no changes

special note: no condensate, since condensate disappeared agar has dulled, overall growth of flat, continous plane, smooth, opaque, yellowish colonies, some areas excluded, there transparent cut outs partially different grow of translucent, frosted glass like colonies

Microbes Me_Development Minutes _c me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

outside storage:

condit. plate no:

01/02_c

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; concealed_covered back locations:

storage notes:

date:

09/02/11

storage outside 11°C (d) 9°C (n); development minutes:

exposed (A) _ no changes

exposed (P) _ no changes

threshold _ no changes

concealed _ no changes

special note: scattered 17 white colonies, spread area 40x40mm, raised elevation, 2.5-3mm diameter, circular form, entire margin, smooth surface, opaque 1 yellow colony, separated, raised elevation, smooth surface, opaque

1mm diameter, circular form, entire margin,


Microbes Me_Development Minutes _c me / site:

body

23/01/11 c. date:

00:54-01:03 c. time:

outside storage:

condit. plate no:

01/02_c

exposed (A)_hand; exposed (P)_face skin; threshold_nose inside; concealed_covered back locations:

storage notes:

date:

09/02/11

storage outside 11°C (d) 9°C (n); development minutes:

exposed (A) _ no changes

exposed (P) _ no changes

threshold _ no changes

concealed _ no changes

special note: scattered 17 white colonies, spread area 40x40mm, raised elevation, 2.5-3mm diameter, circular form, entire margin, smooth surface, opaque 1 yellow colony, separated, raised elevation, smooth surface, opaque

1mm diameter, circular form, entire margin,

11

P01/02_c 09/02/11


form:

elevation:

circular

raised

irregular

convex

undulate

flat

filiform

filamentous

margin:

entire

a slender threadlike object or fiber, esp. one found in animal or plant structures.

umbonate

curled

rhizoid a filamentous outgrowth or root hair on the underside of the thallus in some lower plants, esp. mosses and liverworts, serving both to anchor the plant and (in terrestrial forms) to conduct water.

crateriform

other criteria: size, surface, opacity, colour

lobate

A

COLONY MORPHOLOGY

collection me  

microbes collection me