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International Journal of Environment, Agriculture and Biotechnology (IJEAB)

Vol-4, Issue-3, May-Jun- 2019

http://dx.doi.org/10.22161/ijeab/4.3.9

ISSN: 2456-1878

Table S.2 Composition of MS basal medium (Murashige and Skoog, 1962) INGREDIENTS OF MS MEDIUM Serial No

Macro Nutrients Ingredients

Chemical composition

Weight mg l -1

1

Potassium nitrate

KNO3

23.5 g l-1

2

Ammonium nitrate

NH4 NO3

20.5 g l-1

3

Calcium chloride

CaCL2 2H2 O

5.5 g l-1

4

Potassium diphosphate

KH2 PO4

2.5 g l-1

5

Magnesium sulphate

MgSO4 7H2 O

4.5 g l-1

6

Potassium nitrate

KNO3

23.5 g l-1

Micro Nutrients 7

Boric Acid

H3 BO3

0.31

8

Manganesesulphate

MgSo 4 4H2 O

1.15

9

Zinc sulphate

ZnSo 4 7H2 O

0.430

10

Ferrous sulphate

Fe SO4 7H2 O

1.390

11

Sodium molybdate

Na2 M o O4 2H2 O

0.12

12

Copper sulphate

CuSO4 5 H2 O

1.25

13

Cobalt chloride

CoSo 3 6 H2 O

1.25

Iron source 14

Iron Sulphate

FeSo 4 7 H2 O

27.8

15

Sodium EDTA

Na2 EDTA

1.865

Vitamins 16

Thiamine HCL

17

Myo-inosital

C12 H8 N8 OSCL2

2.0 mg l-1 200 mg l-1

Other Sugar

19

Agar

7.5 g l-1

20

pH

5.8

www.ijeab.com

C12 H22 O11

20 g l-1

18

Page | 659

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