Page 1

Fall 2012

Pennsylvania Highlands Community College New Faculty Orientation Biology Department

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Index

Welcome…………………………………………………………………………..1 Frequently Asked Questions …………………………………………………….2

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WELCOME TO PENNSYLVANIA HIGHLANDS COMMUNITY COLLEGE BIOLOGY DEPARTMENT

Summer 2011 Volume 1, Issue 1

101 Community College Way, Johnstown PA 15904 www.pennhighlands.edu

814-262-6400

Welcome We want to first welcome you to the Biology Department, here at Pennsylvania Highlands Community College. This packet includes information that will be useful in your faculty role as an instructor. This is designed to make your transition into the Biology Department at Pennsylvania Highlands Community College more pleasant and productive. The Biology department provides a solid foundation in science by instilling in students a commitment to search for truth through application of the scientific method, to gain a better understanding of humans, the earth and the universe, and to desire knowledge that can be applied to the service of others. The department offers courses that fulfill the college's Core Curriculum, as well as courses that support the Biotechnology Program. Your role as a member of the department is to aid our endeavors of creating a learning environment that enhances critical thinking, allows for collaboration, and exploration through experience which lends to life-long learning skills.

Wolff, William, Associate Professor, Natural Sciences and Technology Mitchell, Jill, Instructor, Biological Sciences Hollern, Ashley, Instructor, Biological Sciences

Important Phone Extensions    

Security – x 6247 Security Emergency – x 5555 IT Help Desk – x 6470 Front Desk – x 6400

 To make a local call from any room dial 9 first.

Quick Contact List for New Faculty Name William Wolff Jill Mitchell Ashley Hollern

Title Assistant Professor, Chemistry Instructor, Biological Sciences Lead Faculty Instructor, Biological Sciences

Contact 262-6479 262-6487 262-6412

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1. How do I obtain the keys for my classroom? The keys for your lecture are lab room can be obtained from Mary Ann Lee during the hours of 8:30am and 4:30pm. If your class is held after these hours you should contact security at x6427 which can be dialed from any open classroom or from the faculty copy room. 2. Who are the full time departmental employees in biology? Jill Mitchell is the Biology Department lead faculty. She teaches BIO110, and BIO115. Her office is located in C. Ashley Hollern is a full time biology faculty member and teaches BIO210, BIO260, and BIO262. Her office is located in C 3. Who can I ask for assistance while teaching lecture / lab? Feel free to contact either Jill Mitchell at x 6487 or Ashley Hollern at x 6412 with any questions or concerns regarding lab or lecture questions. 4. What supplies are available to me? You will find a list of supplies available to you in this manual. See pages **** 5. Is it ok to borrow supplies from another lab cabinet? We would prefer you obtain the lab supplies from the lab cabinet assigned to your class. However, if supplies are in short supply and you must borrow supplies from another cabinet, please make a note of the need for a purchase order. There is a tablet for “supplies needed” that can be found in C230B Storage room, or C226 B Storage room. 6. Where do I make copies? Copies can be made in the faculty copy room located in C***** 7. Who do I contact if I need additional supplies? If you are aware of the need for additional supplies at the beginning of the semester, you should contact Jill Mitchell with the Supply list, company name and purchase order information so that the order may be processed in a timely manner. 8. How do I address supplies that I run out of during the semester? If you should run out of supplies during the semester and no replacements are available, contact Jill Mitchell ASAP so that an order may be processed. 9. What is the proper PHCC procedure for disposing of laboratory material? You will find a “Lab Safety” section in the manual outlining the proper procedure of handling hazardous materials handled during the semester. 10. Where can I find manuals to the lab equipment available to me? The lab equipment manuals can be found in the C230 A Prep Room, located in the drawer beneath the incubators labeled “Equipment Manuals” 11. How do I address Lab Safety in Lab? At the start of the semester, students should be made aware of proper lab safety protocol. You are required to show a brief video on “Lab Safety” from Flinn Scientific. Following the video ALL students are to receive the “Lab Safety Contract” that they must read and

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sign, prior to the start of any lab. Please keep the contracts until the end of the semester, at which time you must turn them into Jill Mitchell. 12. The lab stations are out of lab disinfectant, where can I find more? The lab countertop disinfectant can be found in the C230 prep room to the left of the dishwasher in a cabinet marked “Lab Disinfectant” 13. Where the emergency gas shut-off located in the lab rooms? The emergency gas shut off is a large red button located near the door. By pushing the button in, the gas is shut “off”. 14. What is the recharging procedure for the chargeable microscopes located in C224/ C226? Microscopes that have been used should be plugged into the outlet strip and the timer knob turned. The timer will turn the charge off automatically after 8 hours. Microscopes that were not used should not be plugged in during this process. Overcharging of the scopes will shorten the life of the battery. 15. Usage of the dishwasher for glassware? What is it primarily used to clean? It can be used to clean glassware, but it is best to have students wash glassware at the end of lab so that glassware remains available to all labs and isn't hanging out in the dishwasher.

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Pennsylvania Highlands Community College


Welcome to The Microbiology Lab C230

The lab and lecture rooms on campus are used frequently for both day and evening classes. Therefore it is of utmost importance that the materials used during class and lecture be returned. ALL supplies and equipment should be put away after use and all glassware needs to be cleaned. You are permitted to prep your materials ahead of time and place the materials on a marked cart in the prep rooms of the lab. However following the lab sections the materials are to be put away. Also, it is your responsibility as an instructor to prep your own labs. As the instructor, you are to plan accordingly to make sure all the materials you need are available prior to the start of lab.

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The following is a list of stocked items, and equipment available for each of the biology courses. If you require additional supplies not present on the list, please contact Jill Mitchell with your order ASAP to assure your purchases are made. In your order supply the company name, item name, item number and quantity required. Note: Not all additional orders are guaranteed and are based on funds available at the time.

BIO 110 Principles of Biology                  

Sugar Glycerin Dish detergent Straws pH paper antacid tablets 3 beverages: i.e. soda, iced tea, Gatorade, etc. Milk String Pennies Ethanol Vegetable oil Lemon juice Ammonia Benedicts solution Iodine solution Buiret solution Sudan IV solution Starch solution

Storage Room C230B                   

Glucose solution Egg white Elodea Agar Corn syrup Syringes Potato cores Dialysis tubing Well plates Sodium bicarbonate Food coloring Yeast Balloons Bromothyml blue Sucrose Prepared mitosis slides Magnetic chromosome kits Electrophoresis gels, etc. Evolutionary agents kit

BIO 115 Principles of Biology II

Storage Room C230B

BIO 210 Microbiology

Storage Room C230B

    

Microscopes Staining trays Test Tube Racks Gloves Prepared Slides o Letter “e” slide o Bacterial Shapes

     

o Gram Stain Glass Slides Cover Slips Rubber Bulb Pippets Toothpicks Oil Stains / Alcohol

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           

o Methylene blue o Crystal violet o Safranin o Nigrosin o Safranin o Methyl red o Iodine o Ethyl alcohol o Acid fast alcohol o Carbol fuchsin o Methyl green o Pond Water Sterile Swabs Bibulous paper Kim wipes Petri plates Test tubes Sterile Cotton Swabs Inoculating Loop Bunsen Burners Hot Plates Clothespins Wax marking pencils

 

cooler unless otherwise noted)

o o o o o o o o o o o  

BIO 260 Anatomy and Physiology I          

Microscopes Dissecting Kits Gloves Synthesizing Macromolecules lab Set Dialysis Tubing Iodine Starch Onion Root Tip / Ascaris Slides Tissue Slides Bones (2 boxes- green, blue)

BIO 262 Anatomy and Physiology II

Microscopes Gloves Dissecting Kits

Alcohol Pads Specimen (All specimen are located in Escherichia coli Staphlococcus aureus Streptococcus pyogenes Clostridium botulinum Bacillus megaterium Corynebacterium diphtheriae Micrococcus luteus Enterobacter aerogenes Serretia marcescens Mycobacterium smegmatis Saccharomyces cerevisiae

What’s in your water Kit Agar: o Nutrient o Eosin Methelyne Blue o Sabouraud agar o MacKonkey agar o Trypticase Soy agar o Nutrient broth

Storage Room C226B          

Skeletons (4) Muscles (see models below) Scapels Mammal Spinal Cord Mammal Brain Mammal Eye Human Senses Lab Kit Phenyl-thiocarbamide (PTC) Thiourea Sodium Benzoate

Storage Room C230B

Blood Group Identification Lab Mammal Heart Double Injected Cats

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Blood Pressure Cuffs Stethoscopes Models: - (2) Human Leg Muscle - (2) Human Arm Muscule - (2) Human Torso - (3) Atlay Human Heart - (3) Human Lung - Kidney Model (Small) - Cross Section With Description chart - Kidney Model (Med) - Cross Section on stand - Kidney Model (Large) - Cross Section with description booklet in base. - Cell Model Globe - Liver Model (Large) Cross Section

-

LAB Equipment

Prep / Storage Room C230A/B

-

Microscopes Balances Hot Plates Stirrers Water Distiller Bunsen Burners Microscopes Autoclave Incubators (2)

-

-

Reflex Arc Model (Large, Flat) Eye Model (small) Eyeball only Eye Model (Medium) in socket with muscle and bone sites Eye Model (Large) in socket with muscle and bone sites (5) Brain Model (small) external colored regions ,and cross section marked sites (4) marked sites cards for identification purposes Male Reproductive Organs in box Brain Model (Large) External and Cross Section with description booklet in base Brain Model in Head (Small)

Fume Hood (2) Dishwasher Cooler Refrigerator Gel Electrophoresis Chambers Micropippeters Thermometers Glassware Test Tube Racks

Note: The lab equipment manuals can be found in the C230 A Prep Room in a cabinet drawer labeled, “Equipment Manuals�.

LAB Equipment C226 -

Prep / Storage Room C226A/B

Beakers Microscopes Yard Sticks Cooler

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Index A. OSHA Laboratory Safety Guidance B. Disposal of Biological Specimen C. OSHA Bloodborne Pathogens and Hazard Communication Standards

A. OSHA Laboratory Safety Guidance Here are a list of helpful links to documents provided by the Occupational Safety and Health Administration. Laboratory Safety Guidance OSHA Fact Sheet OSHA’s Bloodborne Pathogens Standard OSHA Fact Sheet Chemical Hygiene Plan OSHA Fact Sheet Laboratory Safety OSHA Laboratory Standard OSHA Quick Facts Laboratory Safety Autoclaves / Sterilizers OSHA Quick Facts Laboratory Safety Labeling and transferring of Chemicals Other Helpful Links: Material Safety Data Sheets Center for Disease Control and BIO Safety World Health Organization

Management and Disposal of Biological Waste At Pennsylvania Highlands Community College I. Introduction The purpose of this document is to provide information, requirements, guidelines and procedures for the handling and disposal of hazardous and non-hazardous biological waste for all departments and units on the Pennsylvania Highlands Community

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College campus. Currently there are three departments or units that generate biowaste: Science, Healthcare Management, and Workforce Education. “Biological Waste” or “Biowaste” means discarded biological material from teaching and clinical laboratories and operations. This does not include household or office trash. “Biohazardous Waste” means any solid or liquid biological waste that is hazardous because of its physical and/or biological nature. All waste that contains infectious material, or because of its biological nature, may be harmful to humans, animals, plants, or the environment is biohazardous waste. This includes waste from infectious animals, human blood or blood products, pathological waste, and sharps. II. Responsibility Faculty member or other person with operational responsibility 1. Shall be responsible for ensuring students in classes generating biohazard waste comply with the provisions of the plan. 2. Shall take appropriate steps to assure the safety of the students involved in the handling of biowaste. 3. Will take immediate decontamination measures, primarily consisting of intensive flushing, and other preliminary first aid procedures in the event of a spill or other accident, while notifying the Director of Security and Safety or his employees who will in turn notify appropriate medical and HAZMAT first responders.

Science lab manager, Health Care Management appointee, Director of Workforce Education 1. Shall be responsible for the proper storage, handling and packaging for disposal of biological waste generated from their respective programs. Material for disposal shall be clearly marked in the red biohazard bags provided, and stored in the “prep” rooms, room 224A for Healthcare Mgmt. and Workforce Ed., as well as in room 230 for Science, pending pickup. Boxes should also be clearly marked with the name of the originating department for billing purposes. Access to these locked rooms shall be strictly limited to appropriate faculty, or to students under direct supervision of those faculty members, and all access doors will be clearly marked with signs depicting the universal biohazard symbol. 2. Shall notify the Director of Security and Safety when a sufficient number of containers of biowaste or sharps are ready for disposal (2-3 standard boxes) so that a pickup can be scheduled. Will also place orders for replacement containers at this time. Pennsylvania Highlands Community College


3. Shall immediately report spills or other events to the Office of Security and Safety, while ensuring that any necessary decontamination and first aid to affected persons is immediately performed. The Office of Security will respond immediately to the scene of the incident and will coordinate immediate deployment of medical and HAZMAT first responders as appropriate. Facilities Manager 1. Shall be responsible for receiving requested replacement biowaste supplies from the disposal company at the time of a scheduled pick-up and distributing the same to the various departments. 2. Shall be responsible for directing invoices and other paperwork resulting from a pickup to the Director of Security and Safety, who will in turn file or distribute to the appropriate departments as necessary. 3. Shall be responsible for transporting containers of biowaste from the “prep” room to a holding area. This includes assuring that the containers are secure while they await removal by the disposal company. Containers of “sharps” will be transferred to the holding area approximately one hour prior to the anticipated arrival of the disposal company. 4. Facilities Manager will oversee the actual loading and removal of biowaste. Director of Security and Safety 1. Shall assume primary oversight responsibility for procedures as outlined in this plan, as well as ensuring that they conform to applicable state and federal regulations. 2. Shall be notified immediately of any incident occurring on PHCC property involving biowaste spills or other related accidents, particularly those which may result in the contamination of students, faculty, staff or others. Will coordinate notification to appropriate medical and HAZMAT first responders, and will proceed immediately to the accident scene to assist with decontamination and first aid, as well as the evacuation of personnel and securing the area. Subsequent notification of the incident will also be made to the PA Department of Environmental Protection. 3. Following the incident will complete a PHCC Incident/Injury report, and will convene an after-action meeting attended by all involved parties for the purpose of identifying the cause(s) of the accident, as well as making recommendations aimed at avoiding a reoccurrence. 4. Will ensure that all required records and notifications to regulatory agencies are being accomplished correctly and in a timely fashion. Will maintain a file of Department of Environmental Protection (DEP) forms # 2540, Infectious and Chemotherapeutic Manifest, documenting pickup and eventual disposal (i.e. chain of custody).

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5. Upon notification by the referenced departments that a pickup is required, shall contact the disposal company and make arrangements, and that information will be relayed to the departments and to the Facilities Manager. Will also relay orders for replacement biowaste containers as requested from the departments. III. Segregation 1. Any waste that could produce lacerations or puncture injuries must be disposed of as “sharps”. Sharps must be segregated from other waste. Metal sharps and broken glass that pose a biological hazard may be comingled with each other, but not with other waste. Sharps should not be comingled with regular laboratory glassware which has been broken and disposed of in an appropriate and separate container. 2. Biological waste must not be comingled with chemical waste or other laboratory trash. 3. Biohazardous waste should be separated from other biological waste. IV. Containers Containers must be appropriate for the contents; not leak; be properly labeled; and maintain their integrity if thermal or chemical treatment is used. Containers of biohazardous materials must be kept closed. 1. Sharps—use a rigid, puncture-resistant container (heavy-walled plastic is recommended) for storage and disposal. These are usually provided by the disposal company. 2. Solid Biohazardous Waste—Use heavy- duty plastic “Biohazard Bags” (autoclave bags) or containers for biohazardous waste. 3. Solid Non-hazardous Waste—Use heavy-duty plastic bag or other appropriate container without a Biohazard symbol. V. Storage Biological waste may be held temporarily under refrigeration, prior to disposal, in a safe manner that does not create aesthetic problems. Biohazardous waste should be treated and disposed of promptly. Containers holding biohazardous material must be clearly labeled, including the biohazard symbol. Storage of any type of biological waste, as well as any other disposal materials, for the various departments are as follows: Science—the storage or prep room adjacent to Rm C230 Healthcare Management—the smaller storage room off of the large prep room between Rms. C224 and C226. Workforce Education—the large prep room between Rms. C224 and C226.

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VI. Procedures for Treatment and Disposal 1. Microbiological Waste a.) Should be treated (disinfected) promptly on-site by autoclaving at 15 psi and at least 121 °C for at least 30 minutes. Autoclaving is the preferred disinfection method. However, if autoclaving is not possible, microbiological waste may be chemically disinfected by immersion, for not less than three minutes, in a 1:10 (volume/volume) bleach solution. b.) Once treated as above, the waste may be deposited in the regular trash as long as the autoclave bag has been labeled “treated” and then placed in another bag that is opaque—but not a biohazard bag. 2. Animal Carcasses and Body Parts The dissection specimens that the college purchases may be disposed of by way of the regular trash according to the manufacturer. However, it is current policy to dispose of preserved specimens via a disposal company. Therefore, these carcasses and body parts should be handled as follows: a.) During the semester or session, the specimens should be double-bagged, to prevent leakage, and stored in the refrigerator nearest the lab where the dissection is being performed. b.) At the end of the semester, the double-bagged specimens should be placed into a cardboard container, supplied by the disposal company, that is lined with a red biowaste bag. Care should be exercised to not exceed the weight limit of the box, which is typically 50 lbs. In all cases the packaging instructions of the disposal company should be followed (See attached). c.) Notify the Facilities Manager when the package is ready for disposal. 3. Sharps a.) Sharps should be placed into an approved sharps container immediately after use. The container should be kept closed between uses. b.) At the end of the semester or when the sharps container is full, the container should be placed into a cardboard container, supplied by the disposal company, that is lined with a red biowaste bag. In all cases the packaging instructions of the disposal company should be followed (See attached). c.) Notify the Director of Security and Safety when the package is ready for disposal. WEW/9-25-2009

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