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Gluten detection kits Simple ● Specific ● Sensitive Emport, LLC for Biomedal SL


What is Gluten detection? “Gluten� refers to the storage proteins in certain grains. Gluten is composed of several different types of protein, for example wheat contains several different types of gliadins and glutenins.

Antibody

33-mer peptide

Different gluten detection kits use different antibodies to identify components of gluten. However, most components of gluten are actually safe for people with celiac disease! Research indicates that a only very small fraction is toxic for celiacs: the 33-mer peptide of the a-gliadin molecule. The G12 antibody in all GlutenTox kits was specifically developed to detect this toxic peptide. G12 searches for the QPQLPY sequence, found only in the 33-mer peptide. Because of this specificity, G12 is a superior choice for fermented, hydrolyzed, or heattreated foods with complicated molecular structures.


G12, Celiac Disease and Oats Q: Are oats gluten-free? A: Sometimes.

Oats are often subject to cross contamination (in the field and/or during processing). However, just because oats have been grown and processed carefully does not mean they are gluten-free. A 2010 study showed that not all oats are created equal. Different strains of oat have different avenin structures, and only some are toxic to celiacs. The study used the G12 antibody to review several strains of oat: • 3 distinct groups of oat were identified: no, low and high toxicity strains.

• The strains were introduced to cells from celiac patients, and the cells responded appropriately: they only reacted to the toxic oat strains. Accordingly, G12 is the best choice for any company working with gluten-free oats.


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Uses of The GlutenTox line of products is an easy, cost-effective way to: 

Check surfaces in cases of accidental cross-contamination

Verify that sauces/soups/large-batch items are safe for gluten-free diners

Confirm that incoming ingredients are safe (A study published by the American Dietetic Association found that 30% of inherently gluten-free grains found in the supermarket had more than 20ppm gluten (corn flour, rice, millet, etc);

Spot-checking dishes to ensure that staff is appropriately trained in allergen-free preparation

Spot-check and quantify incoming ingredients, batches of product, surfaces, etc.

We want using GlutenTox to be as easy as possible, and will work with you to develop a program that meets your needs.


Biomedal’s GlutenTox Products 

Lateral flow devices (immunochromatographic strips)

Contain the G12 anti-gliadin antibody detecting the immunotoxic 33-mer peptide

Detect gluten in wheat, barley, rye and also oat

No cross-reactivity with soy, corn, rice, quinoa etc.

Test gluten in solid food and liquids

Trace gluten contamination (in food or surfaces)

Long shelf life: 18 months from manufacture

Easy storage and shipping at room temperature (35°F - 85°F)

Certification by AESAN (Spanish Agency for Food Safety and Nutrition, Spanish Ministry of Heath and Social Politics) and the FAPAS Proficiency Test

Biomedal

holds

the

ISO:9001:2008

ISO:17025:2005 (925/LE1568).

(EC-3105/07)

and


Biomedal’s GlutenTox Products • User-friendly gluten detection kit with individually-wrapped tests • Qualitative testing for 20 ppm or 5 ppm • For food and drinks • 10-20 minutes • All equipment included • Easy to use gluten detection kit for commercial kitchens • Semi-quantitative testing at 5 ppm, 10 ppm, 20 ppm, or 40 ppm • For food, drinks and surface testing • 10-20 minutes • All equipment included • Semi-professional gluten detection kit for food manufacturers • Semi-quantitative testing at any gluten threshold (≥3ppm) • For food, drinks and surface testing • 1 hour • Some basic lab equipment required


For celiac patients to avoid accidental gluten ingestion Material provided (no other required, just the sample):

Spoon

Solution 1

Solution 2

Pipettes

Test


Instructions

Available on


For food manufacturers and commercial kitchens to avoid accidental gluten contamination. Suitable for food, liquid and surface testing Threshold (drops of extraction solution needed): 8 drops = 5 ppm 4 drops = 10 ppm 2 drops = 20 ppm 1 drop = 40 ppm

Material provided (no other material required but the sample):

Spoon

Solution 1

Solution 2

Pipettes

25 Tests


Instructions: Food and Drinks


How to use it: Surface Testing


Necessary materials (but non-provided):

Scale Centrifuge or Filters

14 ml Tubes

The most sensitive semi-quantitative rapid test available

Eppendorf Tubes Micropipettes and tips


Instructions Analysis Extraction


How to Surface Swab Test will indicate positive or negative result (cannot be extrapolated in gluten value). A negative result means that 1 kilo of dough, kneaded on this surface will have less than 0.01 ppm of gluten.


Reader Quantitative analysis with :a precise PPM measurement in seconds! • Use a 1:10 dilution ratio with GlutenTox Sticks • Insert test strip into Reader • A precise PPM count appears on the screen in <1 minute • Data is stored on the Reader and easily transferable to any computer via USB


Ordering Info Emport is happy to provide phone or video training on any GlutenTox test kit. Just ask! can be ordered online in increments of 2, 5 or 10 tests, or in bulk by contacting Emport.

&

can be ordered by contacting Emport. Increments of 25 only.

Ask us about setting up an automatic monthly shipment of tests or about alternate quantity needs.


FAQs Q: Are test results valid sooner than 10 minutes / after more than 10 minutes? A: If the sample has a high concentration of gluten, a pink line may appear in only a few minutes. This is a valid result. However, if no pink line appears you must wait the full 10 minutes to declare a truly gluten-free sample.

Test results may shift if left out for too long (positive results fade in intensity and negative results sometimes develop a faint pink positive line), so it’s important to check promptly at 10 minutes. Q: Can you show me some studies?

A: • Gut journal study showing how G12 works with oat • Stanford University study explaining the 33-mer peptide • Stanford University, University of Seville and Biomedal study explaining the G12 and related A1 antibodies

• American Dietetic Association study of inherently gluten-free grains


FAQs Q: Why is the kit not recommended for chocolate? A: Gluten from chocolate or cocoa is very hard to extract because of the phenols present in this matrix: they can chelate gluten. For this reason the level of gluten can be slightly underestimated. However, if you get a positive result with chocolate, it definitely does contain gluten and should not be consumed by celiac disease patients.

Chocolate

is a Sticks kit with a special extraction buffer specifically developed to test cacao or chocolate.

Q: Can the kit be used with fermented, hydrolyzed or baked goods ? A: Yes, the G12 antibody specifically recognizes the fragment that is toxic to celiac patients and can therefore assess toxicity even if the food is fermented, hydrolyzed or heat processed.


FAQs Q: Why is it important to shake the sample vigorously in the extraction stage? A: This stage is crucial because it breaks apart the sample so that the gluten molecules can be isolated / detected. The first step is to grind and homogenize the sample as much as possible. The second step is to shake it, which continues the grinding/breaking-down process. If you need to stop briefly during the 2 minutes of shaking, itâ&#x20AC;&#x2122;s OK. Just make sure the total amount of shaking time is at least 2 minutes.

Q: What do I do with the test once Iâ&#x20AC;&#x2122;ve used it? A: All elements of all GlutenTox tests can be disposed of in ordinary trash, or recycled where appropriate.


FAQs Q: Do I need to purchase separate swabs for surface testing?

A: Nope! The test strip is all you need. Youâ&#x20AC;&#x2122;ll notice that when the strip is faceup, one end has a fuzzy, cotton texture. This end is what gets rubbed directly onto the surface you are testing, and it is also what gets inserted into the dilution solution. One thing to be careful of: always surface test using clean hands (or a glove), to ensure that you do not accidentally contaminate the strip. Q: Can I use GlutenTox Home to surface test? A: We recommend using GlutenTox Pro or GlutenTox Sticks, however in a pinch you can modify GlutenTox Home to surface test. Simply pry open the plastic casing with a thin blade and remove the test strip. You can then treat it like any other surface test.


Links and Contact Info More Info On GlutenTox:

http://www.glutentox.com More Info On Biomedal: http://www.biomedal.com Contact Info:

emilyk@emportllc.com

412.447.1888


Thank you !


About Glutentox 2012