DESIGNING YEAST DERIVATIVE 2.0 Thanks to innovative yeast screening strategy, a new generation of formulated yeast derivatives could be developed that shows promising results in helping livestock animals face stresses, such as pathogen pressure, weaning or feed transition in a context of lower antibiotic usage.
by Bruno Bertaud, Yeast Derivatives Product Manager Lallemand Animal Nutrition and Sylvie Roquefeuil, Communications and PR Manager, Lallemand Animal Nutrition
sample (yeast fraction in our case) and draw the topography of east derivatives (inactivated whole the binding forces (Figure 1, middle images). yeasts or yeast cell walls) are well In the case of yeast, the force studied was the binding force known for their benefits in animal between yeast surface polysaccharides and Type 1 fimbriae. and human nutrition. They are Type 1 fimbriae are adhesion organelles (pili) expressed by particularly used to help balance many gram-negative bacteria (e.g. Coliform, Salmonella, the intestinal microflora and help Campylobacter) and are responsible for the adhesion of these stimulate the host natural defenses. bacteria to the intestinal epithelial cells. Most yeast derivatives on the market today are by-products of the fermentation industry, such as biofuel production. They are Not all yeasts are equal usually characterized according to their biochemical composition: For the first time, we were able to “visualize” very precisely the level of mannan-oligosaccharides (MOS), yeast β-glucans or yeast surface topography in terms of pathogen binding potential. protein contents. If such an approach is interesting to evaluate product purity, it does not totally reflect their functionality. As experts in yeast fermentation, Figure 1: Different Lallemand teams have conducted a yeast strains collaborative research and development exhibit different adhesive capacity. program in partnership with renowned Three steps of research institutes aiming toward a AFM analysis. Left: Microscopic better understanding of yeast fractions. topography of the In particular, the relationship between yeast cell. Middle: composition and function, with a view to representation of adhesion forces design a new generation of specific yeast along the yeast cell fractions with optimal characteristics in (Unit=pNewton). Right: analysis of terms of pathogen binding and modulation binding capacity. of the immune system. Strain 2 shows higher
Looking at yeast fractions at the molecular-level
Cutting-edge techniques such as atomicforce microscopy (AFM) and singlemolecule force spectroscopy (SMFS) were used to study yeast fractions under a totally new light. These techniques represent powerful tools to investigate the forces and motions associated with single molecules. The principle is to use a force sensor (a micrometer-sized bead or a cantilever) to measure the force or tension associated with a biopolymer immobilized on a surface (in our case, the yeast outer cell wall polysaccharides). Hence, the sensor is able to “read” through the surface of the 38 | December 2016 - Milling and Grain