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CAT ALO 1 0 GUE


C ATA L O G U E 2 0 1 0

Ta b l e o f c o n t e n t s IN VIVO STUDIES 2 3 3

Efficacy Testing of Drug Candidates Pharmacokinetic Studies Stereotaxic operations

EX VIVO STUDIES 4 5

Phenotyping studies Morphome TM-Discovery Morphome TM-Junior

TECHNIQUES 6 6 6 7 7 7 8 8 8

Embedding Sectioning Immunohistochemistry Special stains : Golgi-Cox kit In-situ hybridization Laser scanning microscopy Electron microscopy Reporter-Gene assays Apoptosis and cell proliferation assays

MORPHOLOGY 9 9 9-10

X-rays Necropsy Histomorphology

PHYSIOLOGICAL PARAMETERS 11 11 11-12

Urine status Hematology Blood chemistry

GENETIC MONITORING 12

Taq-Man PCR

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SERVICE

NUMBER

2000

IN VIVO STUDIES

SERVICE

NUMBER

2010

E f f i c a c y Te s t i n g of Drug Candidates Frimorfo performs in vivo studies for testing the efficacy of drug candidates in rodent models for various therapeutic areas. Frimorfo scientists work closely together with our clients to choose the best models for the specific compound and the targeted therapeutic area and develop the optimal study plan.

Models

Aphasic distention protocol starting with a 20-s, 1-mL volume distention, followed by 1.5 mL and a 2 mL maximum volume distention. (B) A typical exemple of an EMG tracing after CRD. (Welting et al Neurogastroenterol Motil 17, 838, 2005)

Central nervous system diseases Metabolic diseases Inflammatory diseases Others please inquire

Pain - Examples Neuropathic and inflammatory pain are major areas of unmet need in human medicine. Both can be chronic conditions having a major impact on health and well-being. Frimorfo has validated models in both areas and works with partners to test novel therapeutic agents.

Pain induction (rat) Vaginal distension Colorectal distension

Readout Visceromotor response using implanted EMG electrodes and telemetry to avoid additional stress

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We keep you in focus


SERVICE

NUMBER

2020

Pharmacokinetic Studies Frimorfo performs pharmacokinetic studies in mice and rats Multiple blood samples from one animal Different application routes

Analytics of test compound and metabolites Development of LC-MS/MS method Quantitation of the analytes in serum, plasma and tissues

SERVICE

NUMBER

2030

Sterotaxic operations Frimorfo performs stereotaxic operations in mice and rats for : electrolytic lesions injection of excitotoxic amino acids antero- and retrograde axonal tracing

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EX VIVO STUDIES Phenotyping studies SERVICE

NUMBER

4000

Morphome™-Discovery A comprehensive search for pathologies in your animal model

SERVICE

NUMBER

4200 A minimal number of 1 to 3 control and 1 to 3 experimental animals (homozygous or heterozygous) are required for the Morphome™-Discovery service.

Morphology X-ray analysis -

PAGES

9-10

Physiology -

PAGES

Targeted necropsy of all systems Histomorphology of all systems - more than 50 organs Histopathological diagnosis

11-12 Up to twenty-parameter chemograms Blood count Urine analysis

Our customers receive A detailed report on the analyses A detailed morphological description of phenotypes Representative histological slides of each case Images in electronic format Additional sections or photographs upon request

A morphological analysis of adult mice The Morphome™-Discovery service offers a complete analysis of your preferred animal model.

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SERVICE

NUMBER

4300

M o r p h o m e TM- J u n i o r A morphological analysis of embryos and fetuses A minimal number of 3 control and 3 experimental animals (homozygous or heterozygous) are required for the Morphome™-Junior service.

Morphology Macroscopic inspection -

PAGES

9-10

Microscopic evaluation of whole-mount preparations Histomorphology of embryos in each plane of sectioning

Our customers receive A written report highlighting pathologies and significant structural changes Representative slides of each case Images in electronic format Additional sections or photographs upon request

A morphological analysis of embryos and fetuses The Morphome™-Junior service searches for developmental defects in prenatal specimens, from the 5th day post-coitum to birth.

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SERVICE

NUMBER

6000

TECHNIQUES

SERVICE

NUMBER

6900

Embedding The specimens can be embedded in paraffin for conventional sections or in tissue-tek for cryo sections.

Specimens delivered by the customer Paraffin embedding Tissue-tek Embedding Tissue needs to have been fixed in 4% paraformaldehyde solution or Bouin’s fluid and then stored for transport in 18-20% sucrose, respectively 70% ethanol.

SERVICE

NUMBER

6910

Sectioning Tissue block delivered by the customer Conventional sections (paraffin) Cryo-sections

SERVICE

NUMBER

6920

Immunohistochemistry The Service includes Fixation and dissection of the tissue/organs Embedding and sectioning Secondary antibodies Labeling-kit Immunolocalization (including controls) Pictures

You receive 1. Slides stained with - antiserum - preimmune serum (or secondary antibody) - Hematoxylin&Eosin, Nissl staining 2. digitized photographs

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SERVICE

NUMBER

6930

Special stains Golgi-Cox staining kit A variation of the Golgi techniques which is especially suitable for staining dendritic processes of CNS neurons. Dendritic spines stain strongly, making the morphometric analysis of dendrites and spines of a large number of neurons feasible. Ready to use, four steps method. Each kit contains 3 sets of solutions. The kit is good for 3 rat brains and 6 mouse brains. Frimorfo can perform Golgi-Cox impregnation for clients. Please inquire.

SERVICE

NUMBER

6940

In-situ hybridization The Service includes Fixation and dissection of the tissue/organs Embedding and sectioning Labeling of one probe in sense and antisense orientation In-situ localization (including controls) Pictures

You receive 1. Slides stained with : - antisense probe - sense probe - Hematoxylin&Eosin or Nissl stain 2. Digitized pictures

SERVICE

NUMBER

6950

Laser scanning microscopy The service includes Observation with a LEICA confocal microscope Co-localization studies with up to 3 different markers 3-D reconstruction with the Imaris software

You receive Digitalized pictures and interpretation

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SERVICE

NUMBER

6960

Electron microscopy Transmission (TEM) and Scanning (SEM)

The service includes Embedding in resin (e.g. Epon or Araldite), resp. critical point drying and gold coating Semi- and ultrathin- sectioning Contrasting agents (Uranylacetate, lead citrate) Analysis on a TEM or SEM

You receive 1. Images in electronic format 2. EM-grids 3. Interpretation

SERVICE

NUMBER

6970

R e p o r t e r- G e n e a s s a y s The service includes GFP (Green fluorescence Protein) Lac-Z / Ă&#x;-GAL

You receive 1. Images in electronic format 2. Slides 3. Interpretation For other reporters please inquire.

SERVICE

NUMBER

6980

Apoptosis and cell proliferation assays TUNEL-assay BrdU-assay

You receive 1. Images in electronic format 2. Slides 3. Interpretation

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SERVICE

NUMBER

1000

MORPHOLOGY Special stains upon request or at our discretion depending on the question.

X-rays Necropsy

Visualization of cranial and skeletal anomalies Disclosure of lung anomalies

Integument Cardiovascular Respiratory Immune / hemopoietic Digestive tract Digestive organs Urogenital (female) Urogenital (male) Musculoskeletal Endocrine Central nervous system Peripheral nervous system Sensory organs

Histomorphology Integument STAIN: H&E Skin + appendages Mammary gland

Cardiovascular STAIN: H&E Heart Aorta Inferior vena cava Small vessels

Respiratory STAIN: H&E Nose Larynx Trachea Lung Pleura

Immune / Hematopoietic Bone marrow STAINS: GIEMSA, H&E

Thymus Spleen Lymph node

Digestive tract STAIN: H&E Esophagus Stomach (corpus) Duodenum Ileum Colon Rectum 9


Digestive organs STAIN: H&E Salivary glands Liver Pancreas (exocrine)

Urogenital female / male

STAIN: H&E

Kidney Urinary bladder Ovary Oviducts Uterus Vagina

Kidney Urinary bladder Testis Epididymis / Ductus deferens Prostate Seminal vesicle Penis

Musculoskeletal STAIN: H&E Gastrocnemius / Soleus muscles Tibia Cervical vertebrae

Endocrine STAIN: H&E Pituitary gland Thyroid gland Adrenal gland Pancreas (endocrine) Ovary Testis

Central Nervous System Cerebral cortex STAINS: H&E, NISSL, LUXOL FAST BLUE

Hippocampus Basal ganglia Cerebellum Brain stem Spinal cord

Peripheral Nervous System Sciatic nerve STAIN: H&E, NISSL, LUXOL FAST BLUE

Dorsal root ganglion Trigeminal ganglion Autonomic ganglia

Sensory organs STAIN: H&E Eye Ear Olfactory mucosa Tongue

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SERVICE

NUMBER

8000

Urine status

PHYSIOLOGICAL PARAMETERS Specific gravity Protein - Creatine ratio Sediment Test strip

Hematology Blood counts

Coagulation

Blood chemistry Metabolites

Hematocrit Hemoglobin Erythrocytes Reticulocytes Leukocytes Leukocyte differentiation Platelets Prothrombin time PTT Thrombine time

Chemogram up to 7 parameters (blood from 1 mouse) Chemogram up to 20 parameters (blood from 2 mice)

Bilirubin, total Glucose (serum/plasma) Urea Creatinine Protein Albumin Uric acid Cholesterol Triglycerides Fructosamines Bile acids

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Enzymes

Electrolytes

Alkaline phosphatase (AP) Amylase GLDH ASAT (GOT) ALAT (GPT) γ-GT Creatine kinase Lipase Lactate dehydrogenase (LDH) Sodium (Na) Potassium (K) Chloride (Cl) Calcium (Ca) Magnesium (Mg) Phosphate (P) Iron (Fe) Selenium (Se) + other trace elements upon request We will help you to choose the best parameter constellation.

SERVICE

NUMBER

9000

GENETIC MONITORING Molecular genetic characterization of transgenic animals, includes sample preparation, isolation of the genetic material (DNA and/or RNA) and real-time TaqMan® PCR.

Analysis offered 1. Transgene detection (TaqMan® PCR or SYBR® Green PCR) Transgenic or not? 2. Determination of transgene copy numbers (TaqMan ® PCR) How many copies of the transgene? 3. Zygosity assays (TaqMan® PCR) Homozygous or heterozygous? 4. Gene expression (TaqMan® RT-PCR) 5. Detection of SNP’s or other polymorphisms (TaqMan® PCR) 6. Detection of retroviruses (TaqMan® PCR)

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5

2

4 6 3

9 8

13

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2. Cartilage

10 12 11

1. Trigeminal ganglion 3. Retina and lens 4. Cerebellum - Immuno staining of β-Gal positive cells - Purkinje cells 5. Intestine (Masson-Font coloration) 6. Iris 7. Cerebellum - In-situ Hybridization Purkinje cells calbindin positive 8. Spinal cord 9. Mucous glands of trachea 10. Tooth 11. Bone marrow 12. Testis (MT coloration) 13. Muscle and tendon

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Frimorfo Ltd. Route de l'Ancienne Papeterie P.O. Box 140 CH-1723 Marly 1 Switzerland Phone +41 (0)26 435 7980 Fax +41 (0)26 435 7983 www.frimorfo.com E-mail: office@frimorfo.com


Frimorfo Catalogue