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Research and Development of Agricultural Biotechnology in Nepal Dr. Mukunda Ranjit Academician (RONAST) and General Secretary (NBA)

Introduction  Plant Tissue Culture  Bio-fertilizer  Bio Pesticides  Disease diagnostics  Molecular Characterization  HRD in Biotechnology  Conclusion 

Agriculture Mainstay R&D Ag Biotech  Traditional Biotech 

– – – –

Yeast and Brewery Production Vegetable fermentation Dairy Production Mushroom – Agaricus, Pleurotus, Shitake

Modern Biotech – – –

Plant Tissue Culture Bio Fertilizer Disease Diagnostics & Vaccine Production

Public Institutions



– Botanical


Enterprises – Nepal Biotech Nursery – RLABB – Microplants – GREAT Nepal – HIMBORCE


Plant Tissue Culture Protocols for 100 plant species ď Ź Micro-propagation of virus-free Potato, Banana, Strawberry, Citrus rootstocks, Large Cardamoms, Mums, Orchids etc ď Ź

Potato for elimination of PVA, PVM, PVS, PVX, PVY, PLRV & PSTVd

Banana for elimination of BBTV & CMV

Citrus Rootstocks from Meristem culture

Citrus Micro-grafting For elimination of CTV & CGD

Candidatus Liberibacter asiaticus + CTV

Adult and instars of Diaphorina citri

Seedling Yellows of Eureka lemon Caused by CTV

Citrus Brown Aphid adult & nymphs Toxoptera citricida Vector of CTV


Disease Diagnostics By DAS ELISA •Potato – PVA, PVM, PVS, PVX, PVY and PLRV

•Citrus – CTV: Mexican Lime

•L. Cardamom – Chhirke and Furke: PRSV antibodies

By Biological Indexing and PCR Citrus – CGD: Madame Vinous Sweet Orange and Ponkan Mandarin

Disease Elimination    

Potato viruses eliminated by TT and AMC by PRP, NARC and GREAT PVX and PVY eliminated from Petronese and Multa by GREAT CTV and CGD eliminated from Mandarin by micrografting, GREAT Chhirke and Furke eliminated from RS, GS and DS by GREAT

Bio fertilizers and Bio Pesticides       

RIT for Legumes, RONAST and NARC MIT for composting, RONAST Biogas production from Eupatorium and Water Hyacinth by RECAST Endo and Ecto Mychorrizae forest soil conservation Three BT strains to control lepidepteron pests on crucifer (Pieris brassicae nepalensis ) Biopesticides from Eupatorium, Lantana and Azadirachta

Molecular Characterization Nepal has 0.1% land but 2% flowering plants  700 species of medicinal and aromatic plants  200 species of endemic  MC of Swertia and Camellia  MC of Bacillus thurengenses  MC of Fygopyrum & Oryza sativa 

HRD in Biotech CDB, TU – Post graduate training on TC and plant Biotech  KU/PU – 4 years B.Tech. On Biotech  IAVS – M.Sc. Thesis on Agriculture Biotech 

– Needs Advanced level training (Ph.D., Post

doc) on plant, animal and microbial biotech, immunology and molecular biology

Conclusions: 1. Zero virus PBS production for doubling the yield of potato  2. Production of CTV & CGD-free grafted plants of mandarin to solve the citrus decline problem  3. Aggressive use of bio-fertilizers  4. Production of antibodies and vaccines  5. MC of economically imp. crops 



Dr. Mukunda Ranjit Academician (RONAST) and General Secretary (NBA) Research and Development ofResearchandDevelopmentof Agricultural Biotech...


Dr. Mukunda Ranjit Academician (RONAST) and General Secretary (NBA) Research and Development ofResearchandDevelopmentof Agricultural Biotech...