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Gujarat Board Biotechnology Syllabus for Class 12

Board Sample Paper Gujarat Board Biotechnology Syllabus for Class 12


Time: 3Hours

Total Marks : 70

Unit 1: Protein and Gene Manipulation

Marks 40

Chapter I: Protein Structure and Engineering -Introduction to the world of Proteins -3-D Shape of Proteins -Structure Function relationship in Proteins -Purification of Proteins -Characterization of Proteins -Protein based products -Designing Proteins -Proteomics

15 Marks

Chapter II: Recombinant DNA Technology 15 Marks -Introduction -Tools of rDNA Technology -Making Recombinant DNA -DNA Library -Introduction of Recombinant DNA into host cells -Identification of recombinants -Polymerase Chains Reaction (PCR) -DNA Probes -Hybridization Techniques -DNA Sequencing -Site-directed mutagenesis Chapter III: Genomics and Bioinformatics 10 Marks -Introduction -Genome Sequencing Projects -Gene Prodiction and counting -Genome similarity, SNP's and comparative genomics -Functional Genomics -History of Bioinformatics -Sequences and Nomenclature -Information Sources -Analysis using Bioinformatics tools. Unit II : Cell Culture Technology Chapter I: Microbial Culture and Applications -Introduction -Microbial Culture Techniques -Measurement and Kinetics of microbial Growth -Scale up of microbial process -Isolation of microbial products -Strain isolation and Improvement -Applications of microbial culture technology -Bio safety of genetically modified micribes Chapter II: Plant Cell Culture and Applications 10 Marks -Introduction -Cell and Tissue Culture Techniques -Applications of Cell and Tissue Culture -Gene Transfer Methods in Plants -Transgenic Plants with Beneficial Traits -Diagnostics in Agriculture and Molecular Breeding -Bio safety of genetically modified plants Chapter III: Animal Cell Culture and Applications 10 Marks -Introduction -Animal Cell Culture Techniques -Characterisation of Cell Lines -Scale-up of Animal Culture Process -Applicationsof Animal Cell Culture -Stem Cell Technology

Tamilnadu Board Class 12 Geography Sample Paper Of 2011 CBSE Board Class 9 Science 2005 CBSE Board Class 12 Physics 2005 CBSE Board Biology Sample Papers for Class 12 for year 2011 Gujarat Board Class 12 Geography 2007 Tamilnadu Board Class 12 Home Science Sample Paper Of 2011 West Bengal Board Class 11 Biology Sample Paper Of 2012 ICSE Board Class 10 Computer Science 2010 CBSE Board Class 9 Math 2009 Andhra Pradesh Board Class 12 Chemistry 2011 Previous Year Paper

10 Marks

CBSE Board Class 12 Psychology 2008 CBSE Board Class 12 Accountancy 2010 CBSE Board Class 11 Functional English 2007 CBSE Board Class 12 English Elective 2005 CBSE Board Class 11 Economics 2011 CBSE Board Class 12 Economics 2011 CBSE Board Class 11 Business Studies 2009 CBSE Board 12 Biology Previous Year Question Paper 2007 CBSE Board Class 11

-Bio safety of genetically modified animals Practical's Note: Every student will be required to do the following experiments during the academic session List of Experiments 1. Isolation of bacterial plasmid DNA and its detection by gel eletrophoresis

Functional English 2005 CBSE Board Class 12 Functional English 2011

2. Restriction digestion of plasmid DNA and its analysis by gel electrophoresis 3. Bacterial transformation using any plasmid


4. Data retrival and data base search using internet site NCBI

Gujarat Board Class 9 Social Science CBSE Board Class 9 Science Andhra Pradesh Board Class 12 Economics ICSE Board Class 10 Home Science Andhra Pradesh Board Class 11 Physics West Bengal Board Class 12 Syllabus For Accountancy West Bengal Board Class 11 Syllabus For Chemistry West Bengal Board Class 11 Syllabus For Political Science ICSE Board Class 9 Biology Andhra Pradesh Board Class 12 Syllabus of Math

5. Download a DNA and protein sequence from internet, analyse and comment on it. 6. Cell viability assay (using Evans blue Stain) 7. Determination of blood groups. 8. Estimation of DNA 9. Ion-exchange chromatography for proteins. 10. Reading of a DNA sequencing gel and arrive at the sequence. 11. Estimation of blood glucuse by enzymatic method (GOD/POD) 12. Project work.

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