Safety culture is the way in which organizations live and breathe safety. If there is to be improvement in the quality of healthcare, the assessment of safety culture is paramount. In this position paper, we make the case for using a mixed methods approach, in which quantitative and qualitative methods are combined, to improve the accuracy of results for when a healthcare organization’s safety culture is assessed. The approach has been tested in healthcare settings in several countries. This position paper describes practical steps for the assessment of safety culture in healthcare using mixed methods, and how best to use the results and achieve maximum value for organizational learning.