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CATALOG 2010 Fixation Processing Embedding Sectioning Staining Storage Instruments


Histo-Line Laboratories Srl is proud to present the New 2010 Histology Catalog as a valuable tool for your clinical diagnostic and research needs. For additional information please visit our website www.histo-online.com and www.histoline.com


Table of contents

Fixation

4

Processing

6

Embedding

8

Sectioning

10

Staining

11

Storage

20

Instruments

22

3


Fixation

Description

Cat.

Quantity

B5 solution

07-119

6 x 500 ml

Bouin solution

07-116

6 x 500 ml

Bouin Holland liquid

07-117

6 x 500 ml

Cyto Spray Cytological Fixative non aerosol

R0150

12 x 250 ml

04018-1 04018-4

1 litre 4 x 1 litre

Glutaraldehyde, Biological grade 25%

00376-500 00376-4

500 ml 4 x 500 ml

Glutaraldehyde, Biological grade 50%

00377-500 00377-1

500 ml 1 litre

Glutaraldehyde, EM grade 8%

07710-100 07710-5 00216-30

100 ml 5 x 100 ml 30 x 10 ml

Glutaraldehyde, EM grade 25%

01909-100 01909-5 01909-10

100 ml 5 x 100 ml 10 x 10 ml

Glutaraldehyde, EM grade 50%

18428-100 18428-5 18428-10

100 ml 5 x 100 ml 10 x 10 ml

Glutaraldehyde, EM grade 70%

01201-2 01201-5

10 x 2 ml 5 x 10 ml

Cyto-Spray is a mixture of ethanol and carbowax specifically formulated to assure rapid, high quality of cytological samples

Formaldehyde Ultra Pure methanol free, EM grade Formaldehyde, 10%, methanol free, Ultra Pure[50-00-0] -Suitable for both electron and light microscopy - Easily penetrates large blocks of tissue - When used in combination with glutaraldehyde, it fixes delicate tissues such as brain in vascular perfusion - Avoids the problem of having to depolymerize paraformaldehyde - Used in Karnovsky's fixative in conjunction with your own buffer system.

Biological Grade Glutaraldehyde has been purified to minimize by-product formation and maximize shelf life. It should be suitable for most morphological studies

4


250 ml 500 ml

Histo-Fix Formalin 10% V/V

DR001 DR002 DR003

4 x 5 litres 10 litres 20 litres

Ice Spray

R0020

12 x 150 ml

Liquid of Carnoy

15-104

250 ml

Paraformaldehyde EM Grade

00380-1 00380-250

1 Kg 250 g

15-123

500 ml

Preserve Your Specimens "To Go" PolyTransport Buffer is a medium used to transport and preserve specimens from surgery to the point of grossing for fixation prior to further processing. The specially formulated transport medium is designed to preserve tissue in a viable state during transport by maintaining osmolarity and pH for 24 to 48 hours.

Fixation

24311-250 24311-250

Histocon PolyTransport Buffer

Freezing Spray aereosol

Paraformaldehyde depolymerizes in water to formaldehyde solution yielding consistent quality fixative solutions. To achieve a strong solution, raise the temperature of the water to 60째C then add sodium hydroxide solution dropwise. Karnovsky, J. Cell Biology, 27, 137A (1965)

Zenker Solution

5


Cat.

Quantity

Blue

R4004

1000 pcs

Grey

R4005

1000 pcs

Green

R4003

1000 pcs

Pink

R4002

1000 pcs

White

R4172

1000 pcs

Yellow

R4001

1000 pcs

White

RBPW

500 pcs

Blue

RBPB

500 pcs

Citro-Histoclear

4 x 5 litres

Clearing agent for Histology, D-Limonene

R0050CITRO

Decalc

R0060

6 X 1 litre

R0055/20

4 x 5 litres

Description Biopty Cassettes

Processing

Disposable cassettes for processing and embedding biopsy

Biopsy Pad

For rapid decalcification with superior staining qualities. Histo-Decalc is a rapid bone and tooth decalcifier for the preparation of small histological materials with improved tissue preservation and superior staining qualities. Decalc represents a major advance in histological technique. The stained preparation shows excellent histological details

Diaphane Clearing reagent

6


Embedding Cassettes R4157

1000 pcs

Green

R4156

1000 pcs

Pink

R4155

1000 pcs

White

R4170

1000 pcs

Yellow

R4154

1000 pcs

Ice Spray

R0020

12 x 150 ml

3410/20

4 x 5 litres

Processing

Blue

Freezing Spray aereosol

Xilene Histo Grade Xilene clearing agent for histology

7


Description

Cat.

Quantity

Base Molds 7x7x5mm

R4161

12 pcs

Base Molds 15x15x5mm

R4162

12 pcs

Base Molds 24x24x5mm

R4163

12 pcs

Base Molds 30x24x5mm

R4164

12 pcs

Base Molds 37x24x5mm

R4165

12 pcs

Base Molds 37x24x12mm

R4166new

12 pcs

Blue

R4004

1000 pcs

Grey

R4005

1000 pcs

Green

R4003

1000 pcs

Pink

R4002

1000 pcs

White

R4172

1000 pcs

Yellow

R4001

1000 pcs

Blue

R4157

1000 pcs

Green

R4156

1000 pcs

Pink

R4155

1000 pcs

White

R4170

1000 pcs

Yellow

R4154

1000 pcs

Base Molds Molds in stainless steel for blocks of paraffin, available in 6 different sizes according the size of the samples. Can be used with Mono Cassettes, Biopty Cassettes and Embedding Rings

Embedding

Biopty Cassettes Disposable cassettes for processing and embedding biopsy

Embedding Cassettes

8


Embedding Rings Embedding Rings are made in ABS plastic material that is heat and compression resistant. ER make it possible to obtain uniform blocks of paraffin and can be set up on any type of Microtome R4013

1000 pcs

Green

R4012

1000 pcs

Pink

R4011

1000 pcs

White

R4151

1000 pcs

Yellow

R4010

1000 pcs

Lab-O-Wax 56-58 °C

R0040

6 x 2 Kg

R0040-PLUS

6 x 2 Kg

R0042

6 x 2 Kg

Mega Cassettes - White

R4173

1000 pcs

Super Mega Cassettes – White

R4173

100 pcs

Embedding Medium for histological use

Lab-O-Wax PLUS 56-58 °C

Embedding

Blue

Lab-O-Wax Plus is a superior embedding media Lab-O-Wax Plus is a mixture of pure paraffin and plastic polymers, compression of tissue due to cutting is reduced to a minimum.

Lab-O-Wax 52-54 °C Mega Embedding Cassettes Cassette for processing and embedding big tissue specimens, is of heat, solvents, decalcification agents and ultrasound proof, plastic material construction.

MEGA

O.C.T. Compound

R0030

12 x 125 ml

9


Disposable Blades

Description

Cat.

Quantity

Disposable Blades Type A35

R4694

50 pcs

R4689

20 pcs

R4691

50 pcs

R4695

50 pcs

Description

Cat.

Quantity

Aqueous Mount

HLAMT030 HLAMT060 HLAMT500

30 ml 60 ml 500 ml

FluoreGuard Mounting Medium

HLFMM030 HLFMM060 HLFMM500

30 ml 60 ml 500 ml

Permanent Mounting Media (Aqueous)

HLPMT030

30 ml

Pertex Mounting Media (Xylene-based)

R0081

6x500 ml

For thin sectioning of paraffin embedded tissue blocks

Disposable Blades Type C35 For sectioning of tissue on cryostat

Disposable Blades Type N35 For sectioning of hard samples

Sectioning

Disposable Blades Type S35 For thin sectioning of paraffin embedded tissue blocks

Mounting Media

10


STAINING SOLUTIONS Description

Cat.

Quantity

EA 50

R0160

6 x 1 litre

Eosin alcoholic

R0190

6 x 1 litre

Eosin aqueous

R0200

6 x 1 litre

Giemsa

R0210 R0215

6 x 500 ml 6 x 1 litre

Haematoxylin Harris

R0180

6 x 1 litre

Haematoxylin Mayer

R0182

6 x 1 litre

May-Grunwald

R0220 R0225

6 x 500 ml 6 x 1 litre

OG2

R0165

6 x 1 litre

OG6

R0170

6 x 1 litre

Staining 11


SPECIAL STAIN KITS

Description

Cat.

Quantity

Kit AgNOR

14-100

1 kit

14-102

1 kit

14-103

1 kit

14-104

1 kit

14-127

1 kit

Method to detect the argentaffin proteins associated to the nucleolar organizing region (NOR).

Kit Alcian blue pH 2,5 Method for the specific demonstration of acid mucopolysaccharides in tissue sections.

Kit Alcian blue PAS

Staining

Method for the simultaneous demonstration of acid mucins, alcian blue positive, neutral mucopolysaccharides and carbohydrates, PAS positive, in tissue sections.

Kit Azan trichrome Method for the demonstration of muscle fibers, glial fibrils, collagen, reticulum, glomerular stroma of the kidney, erythrocytes and nuclear chromatin in tissue sections.

Kit Congo red Method for the demonstration of amyloid in tissue sections. It shows a high birefringence under polarized light.

12


Kit Diastase digestion method

14-105

1 kit

12-103

1 Kit

14-108

1 kit

14-109

1 kit

14-110

1 kit

The digestion on histological section by diastase solution is indicated when the glycogen masks the epithelial mucins. It is a method recommended for liver biopsy

Fast color kit (fast staining of blood smear) Rapid method for differential stain of blood figured elements. Can be used for staining slides of: cytological, fine-needle, cytocentrifuge, malaria..

Kit Feulgen Method for the specific demonstration of DNA in tissue section.

Method for the simultaneous demonstration of bilirubin and collagen in tissue sections.

Kit Fuchsin paraldehyde Gomori

Staining

Kit Fouchet Van Gieson

Method for the demonstration of elastic fibers, alpha and beta cells of islets of Langherans, mast cells granules of gastric mucosa, argentaffin cell granules on tissue sections.

13


Kit Gram

14-111

1 kit

14-112

1 kit

14-113

1 kit

14-115

1 kit

14-116

1 kit

For the simultaneous demonstration of Gram negative and Gram positive bacteria on histological section and smear.

Kit Grimelius Method for the demonstration of argyrophil substances on cells and tissues.

Kit Grocott Method used for the demonstration of certain fungi in tissue section.

Staining

Kit Luxol fast blue Kluwer Barrera Method for the demonstration of myelin and phospholipids on section of central nervous system.

Kit Mallory trichrome Method for the demonstration of collagen, reticulum, cartilage, bone, amyloid and nuclei in tissue sections.

14


Kit Masson Fontana

14-117

1 kit

14-118

1 kit

14-119

1 kit

14-120

1 kit

14-130

1 kit

Method for the demonstration of melanotic pigment on histological section.

Kit Masson trichrome Method for the demonstration of connective tissue; in detail: gametes, nuclei, nevroglia, neurofibrils, collagen, keratin, intracellular fibrils and the negative image of the Golgi apparatus in tissue sections.

Kit Masson trichrome Goldner

Kit May Grunwald Giemsa

Staining

Method for the demonstration of connective tissue; in detail: gametes, nuclei, nevroglia, neurofibrils, collagen, keratin, intracellular fibrils and the negative image of the Golgi apparatus in tissue sections.

Method for the differentiation and demonstration of cell type and parasites, bacteria, rickettsiae, inclusion bodies, mast cell granules and cellular typing and endothelial reticulum in tissue sections.

Kit Methyl Green pyronin Method for the demonstration of DNA and RNA in tissue sections. It is useful also for the demonstration of RNA and plasma-cells on histologic and cytological samples.

15


Kit Nissl for neurons

12-128

1 kit

Kit Oil red O

14-146

1 kit

14-153

1 kit

14-122

1 kit

14-123

1 kit

12-106

1 kit

Method used for neutral lipid demonstration

Kit Orceine for elastic fibres Method for the identification of elastic fibers on tissue section.

Kit PAS Hotchkiss McManus Method for the demonstration of tissue components characterized by adjacent glycol or aminohydroxyl groups.

Staining

Kit PAS Morel Maronger Method for the demonstration of neutral mucopolysaccharides and connective tissue components.

Kit Perls Method for the demonstration of reactive ferric iron on tissue sections.

16


14-124

1 kit

14-125

1 kit

14-107

1 kit

14-128

1 kit

Kit Sudan black

12-105

1 kit

Kit Van Gieson trichrome

14-129

1 kit

Kit Perls Van Gieson Method for the demonstration of reactive ferric iron, collagen and connective on tissue sections

Kit Picromallory For the demonstration of the different connective components.

Kit PTAH phosphotungstic haematoxylin The method is useful in order to differentiate the skeletal muscle from the smooth one: it stain the isotropic bands of the . skeletal muscle. It points out also the fibrin.

For the demonstration of tissue argyrophil tissue components, glycogen and mucines. It is widely used for the demonstration of certain fungi in tissue section. It is an elective method for the study of basement membrane in kidney biopsy.

Staining

Kit Silver methenamine P.A.S.M

Method for the demonstration of collagen fibers, discriminates them from connective tissue.

17


Kit Von Kossa

14-131

1 kit

14-132

1 kit

14-133

1 kit

14-134

1 kit

14-135

1 kit

Method for the demonstration of anions of calcium salts in tissue section.

Kit Warthin Starry Method for the demonstration of spirochete on tissue section.

Kit Weigert's long method For the demonstration of elastic fibers in tissue sections. This method uses overnight staining and it is preferred for very fine fibers staining.

Staining

Kit Weigert's fast Method for the demonstration of elastic fibers in tissue sections. This short method has a lower specificity: also collagen and basal membrane could be stained.

Kit Weigert's Van Gieson The stain method is particularly useful for the demonstration on the same slide of elastic fibers, connective tissue, collagen and nuclei. It is suitable for vascular pathology studies.

18


Kit Weigert's Van Gieson fast

14-136

1 kit

14-137

1 kit

14-138

1 kit

Method for the demonstration of elastic fibers in tissue sections, many variants exist of the original Weigert’s method, this short method has a lower specificity: also collagen and basal membrane could be stained.

Kit Ziehl-Neelsen Method for the demonstration of pathological mycobacteria in tissue section, in sputum or in culture media smears. Particularly for Kock’s and Hansen’s bacteria.

Kit Ziehl-Neelsen Fite

Staining

Method for the demonstration of pathological mycobacteria in tissue section, in sputum or in culture media smears. Particularly for Kock’s and Hansen’s bacteria.

19


FILING SYSTEMS FOR PARAFFIN BLOCKS

Description

Cat.

Qty

Filing Cabinet WHITE in METAL for paraffin blocks

R4192

1

Filing Cabinet WHITE in ABS for paraffin blocks

R4192-ABS

1

Filing Cabinet BLUE in ABS for paraffin blocks

R4192A-ABS

1

Filing Cabinet GREEN in ABS for paraffin blocks

R4192G-ABS

1

Filing Cabinet HAVANA in ABS for paraffin blocks

R4192H-ABS

1

Filing Cabinet PINK in ABS for paraffin blocks

R4192R-ABS

1

Filing Cabinet YELLOW in ABS for paraffin blocks

R4192Y-ABS

1

Filing Cabinet for Macro Cassettes

R4192/MACR O

1

Base stand for Filing Cabinet

R0549

1

Top Cover for Filing Cabinet R41 and R054

R0548

1

Storage 20


FILING SYSTEMS FOR SLIDES

Description

Cat.

Quantity

Filing Cabinet WHITE in METAL for slides

R0540

1

Filing Cabinet WHITE in ABS for slides

R0540-ABS

1

Filing Cabinet BLUE in ABS for slides

R0540B-ABS

1

Filing Cabinet GREEN in ABS for slides

R0540G-ABS

1

Filing Cabinet HAVANA in ABS for slides

R0540H-ABS

1

Filing Cabinet PINK in ABS for slides

R0540R-ABS

1

Filing Cabinet YELLOW in ABS for slides

R0540Y-ABS

1

Base stand for Filing Cabinet

R0549

1

Top Cover for Filing Cabinet

R0548

1

Storage 21


TEC-2800

Embedding Center

Characteristics: Working surface The tissue embedding cassettes and base molds trays are removable and can be set at different temperatures The heated forceps holder can be set at the preferred temperature. The ergonomic large working surface allows processing many samples at the same time The setting knobs allow to adjust the precise paraffin flow Cold Spot The large cold spot Peltier is designed to orient even small and big samples (Mega Cassettes) The temperature range is from room temp. to -5 째C The large work surface allows draining of the excess paraffin into two large collecting trays and allows processing many samples at the same time The temperature can be set from ambient to +99 째C and can be adjusted through the control panel Control Panel All functions of the TEC 2800 are settable through the control panel Temperature of working surface Temperature of molds cassettes warming trays Temperature of paraffin tank Work time and day can be programmed automatically

Instruments

Nominal supply voltage Maximum power draw Working temperature increments Capacity Paraffin reservoir Cassettes/ molds tray Dimensions Width Depth Height Weight

220 V/50 Hz 1000 VA from ambient to 70 째C 1째C 4,5 Lt 1,4 Lt 560 mm 640 mm 400 mm 26 Kg

22


TEC 2800 Cryo Console The TEC 2800 Cryo Console is composed of a compressor cooled work surface held to chill the paraffin blocks rapidly. The Teflon coated surface allows a minimum ice formation and easy cleaning. Temperature is adjustable from ambient to -13 °C. TEC 2800 Cryo Console can be used as a stand alone unit near the microtome station. Nominal supply voltage Maximum power draw Working temperature increments Dimensions Width Depth Height Weight

220 V/50 Hz 110 VA from ambient to -13 °C 1 °C 340 mm 600 mm 390 mm 24 Kg

TEC 2800 Thermal Console The TEC 2800 Thermal Console holds processed tissue specimens, in any type of cassettes, awaiting paraffin embedment. Stores and warms samples in a large paraffin tank “2,5 Lt.” Temperature can be set from ambient to 99 °C. 220 V/ 50 Hz 400 VA from ambient to 70 °C 1 °C 2,5 Lt 300 mm 540 mm 390 mm 7,5 Kg

TEC 2900 WaxTrim The TEC 2800 Histo Trim is a practical device designed to remove excess of paraffin from the embedding cassettes after inclusion of specimens. Scraping the embedded block on the “grooved plane” heated surface, excess paraffin will be removed leaving the cassette clean ready to be set properly on the clamp of the microtome. Temperature can be set from ambient to 99 °C.

Instruments

Nominal supply voltage Maximum power draw Working temperature increments Capacity Paraffin tray Dimensions Width Depth Height Weight

23


ARM 3600

Fully Motorized Programmable Rotary Microtome

Function and characteristics: Motorized drive Compatible Embedding Material: paraffin, plastic The cutting and trimming function can be finished by the control system The hand wheel can be locked at any position, so as to ensure the safety of the operation The large waste tray can be exchanged arbitrarily Two kinds of the specimen clamps, standard and universal cassette can be changed easily Two models for section: Manual and automatic, the user can switch arbitrarily. Switch arbitrarily between section and trimming Speed control through the knob for enhanced efficiency Intuitive control panel Section thickness totalized and section counter New blade holder with finger safe Wide range of accessories

Standard delivery: Standard delivery include:

Instruments

1 ARM 3600 basic instrument 1 section waste tray 1 disposable blades holder 1 universal fast clamp for cassette 1 standard clamp for blocks 1 operating manual 1 bottle (50 ml) of oil for drives 1 box of 50 disposable blades type S35 Do not include: 1 holder for standard reusable knife 1 stainless steel knife

24


Technical Parameters: Approvals:

CE IVD

Room conditions Room temperature range: Relative humidity:

+ 15 °C to + 35 °C max. 80% , non condensing

Microtome Section thickness setting:

0,5 - 100 µm 0 to 10 µm in 0,5 µm increments 10 to 20 µm in 2 µm increments 20 to 100 µm in 5 µm increments 1 – 100 µm 1 to 10 µm in 1 µm increments 10 to 20 µm in 2 µm increments 20 to 50 µm in 5 µm increments 50 to 100 µm in 10 µm increments

Maximum section of slice: Slice precision:

50×50mm ±10%

Horizontal specimen stroke: Vertical specimen stroke: Retraction of the specimen clamp: Orientation of the specimen head:

26 mm 60 mm 20 m 360°

Dimensions Width (with handwheel): Width (without handwheel): Depth (with waste tray): Depth (without waste tray): Working height (knife edge): Working height (knife edge): Height (total): Weight (without accessories):

413 mm 300 mm 618 mm 520 mm 100 mm 168 mm 305 mm (with storage area on the housing) approx. 37 kg

Instruments

Trimming section thickness setting:

25


MRS 3500

Motorized Semiautomatic Utra-Thin Microtome

Function and characteristics: Motorized drive Compatible Embedding Material: paraffin, plastic. The cutting and trimming function can be finished by the control system The hand wheel can be locked at any position, so as to ensure the safety of the operation The large waste tray can be exchanged arbitrarily Two kinds of the specimen clamps, standard and universal cassette can be changed easily Switch arbitrarily between section and trimming Intuitive control panel Section thickness totalize and section counter New blade holder with finger safe Wide range of accessories

Standard delivery: Standard delivery include:

Instruments

1 MRS3500 basic instrument 1 section waste tray 1 disposable blades holder 1 universal fast clamp for cassette 1 standard clamp for blocks 1 operating manual 1 bottle (50 ml) of oil for drives 1 box of 50 disposable blades type S35 Do not include Optional: 1 holder for standard reusable knife 1 stainless steel knife

26


Technical Parameters: Approvals:

CE IVD

Room conditions Room temperature range:

+ 5 °C to + 40 °C

MRS3500 Section thickness setting:

0,25 - 60 µm 0.25 to 1 µm in 0.25 µm increments 1 to 10 µm in 1 µm increments 10 to 20 µm in 2 µm increments 20 to 60 µm in 5 µm increments

Horizontal specimen feed: Vertical stroke: Specimen orientation (optional) Horizontal: Vertical:

28 mm 60 mm

Maximum specimen size:

W 50 x H 66 x D 30 mm

Dimensions Width (with handwheel): Depth (with waste tray): Height: Weight (without accessories):

305 mm 445 mm 310 mm 26 kg

± 25 mm ± 20 mm

Instruments 27


MR 2258

Manual Rotary Microtome

Function and characteristics: Compatible Embedding Material: paraffin, plastic The hand wheel can be locked at any position, so as to ensure the safety of the operation The large waste tray can be Exchanged arbitrarily Two kinds of the specimen clamps, standard and universal cassette can be changed easily New blade holder with finger safe Wide range of accessories

Standard delivery: Standard delivery include: 1 MR 2258 basic instrument 1 section waste tray 1 disposable blades holder 1 universal fast clamp for cassette 1 standard clamp for blocks 1 operating manual 1 bottle (50 ml) of oil for drives 1 box of 50 disposable blades type S35 Do not include Optional:

Instruments

1 holder for standard reusable knife 1 stainless steel knife

28


Technical Parameters: Approvals:

CE IVD

Room conditions Room temperature range:

+ 5 °C to + 40 °C

MR2258 Section thickness setting:

1 - 60 µm 0,5 to 2 µm in 0,5 µm increments 2 to 10 µm in 1 µm increments 10 to 20 µm in 2 µm increments 20 to 60 µm in 5 µm increments 25 mm ±1 mm 60 mm 8° 8° ± 90 °

Dimensions Width (with handwheel): Depth (with waste tray): Height: Weight (without accessories):

400 mm 600 mm 300 mm 34 kg

Instruments

Horizontal specimen feed: Vertical stroke: Specimen orientation (optional) Horizontal: Vertical: Rotability:

29


MC 4000

Microtome Cryostat Dual Compressor UVC Lamp Decontamination Automatic Defrosting

Function and characteristics: Motorized drive Chamber temperature down to –30°C Dual Compressor unit for rapid freezing down to –50°C Automatic and manual defrosting The cutting and trimming function can be finished by the control system Holder for disposable blades with adjustable cutting angle Anti roll device The hand wheel can be locked at any position, so as to ensure the safety of the operation Switch arbitrarily between section and trimming Intuitive control panel Section thickness totalize and section counter New blade holder with finger safe Wide range of accessories

Standard delivery include:

Instruments

1 MC 4000 basic instrument 1 section waste tray 1 disposable blades holder 5 Specimen discs 1 Heater extractor 1 operating manual 1 box of 50 disposable blades

30


Technical Specifications: Section thickness setting

1-60 µm 0-10 µm in 1 µm increments 10-20 µm in 2 µm increments 20-60 µm in 5 µm increments

Maximum specimen size: Horizontal specimen feed: Vertcal specimen stroke: Specimen orientation : Trimming:

50x70 mm 28 mm 59 mm 8° (x,y,z axis) motorized coarse feed

Motorized coarse feed: Rapid: Slow:

2 speeds 0,8mm/sec 0,2 mm/sec

Dimension: Width (excluding handwel): Width (including handwel): Depth: Height: Weight:

705 mm 760 mm 640 mm 1115 mm 152 Kg

Instruments

Cooling capacity: Temperature setting range: 0°C to –35°C Cooling down time to –35°C appr. 4 hours Chamber defrosting programmable or manual 1 automatic defrosting cycle / 24 hour duration : 9 min. Freeze shelf temperature -40°C Nominal supply voltage: 220 V / 50 Hz 110 V / 60 Hz Maximum power draw: 450 VA

31


MC 5000

Microtome Cryostat Dual Freezing System UVC Lamp Decontamination Automatic Defrosting

Function and characteristics: Motorized drive. Chamber temperature down to –35°C Double Peltier unit for rapid freezing down to –50°C Automatic and manual defrosting The cutting and trimming function can be finished by the control system. Holder for disposable blades with adjustable cutting angle Anti roll device The hand wheel can be locked at any position, so as to ensure the safety of the operation. The specimen clamp can be oriented on the X—Y axis Section waste aspiration system. Switch arbitrarily between section and trimming. Intuitive control panel Section thickness totalize and section counter New blade holder with finger safe Wide range of accessories

Standard delivery include:

Instruments

1 MC 5000 basic instrument 1 section waste tray 1 disposable blades holder 5 Specimen discs 1 Aspiration hose 1 Heater extractor 1 operating manual 1 box of 50 disposable blades

32


Technical Specifications: CE IVD

Microtome: Section thickness range: Increment: Trimming thickness range: Increment: Specimen retraction: Horizontal stroke: Vertical stroke: Specimen orientation:

1-60 m Adjustable 1 m, Increment: ±20% 10 - 300 m Adjustable 10 m, Increment:±20% 20 m 25mm 45mm 8 X,Y,Z three directions

Cryostat Voltage: Frequency: Power: Chamber temperature: Freezing shelf temperature: Number of freezing station: Peltier number: Refrigerant: Compressor oil:

220V±10% 50Hz 850 V/A -10 - -35 Adjustable -50 18 2 R404a,300g±10g 0.6L EMKARATE RL-22S, ICI

Dimensions: Width (no hand wheel): Width With hand wheel): Depth: Height: Weight:

680 mm 826 mm 700 mm 1150 mm 126 kg

Instruments

Approvals:

33


ATP 700

Automatic Tissue Processor

Function and characteristics: Compact, bench top, linear tissue processor designed to process biological tissues from fixation to paraffin infiltration The unique linear design offers reliable technique in a small space in the laboratory Histo Line ATP 700 includes 14trays, 10 reagents and 4 paraffin can process 2 x 75 samples simultaneously All the reagent and paraffin containers are completely closed to prevent evaporation Electrostatic filter prevent solvent odors and pollution ATP 700 can be programmed for immediate or delayed start The immersion time can be set from 1 minute to 99 hours and 59 minutes

Instruments 34


Technical Specifications: 14 4 6 150 1500 ml

Setting range time for each step: Temperature range for paraffin:

From 1 min. to 99 h 59 min. From 50째C to 89째C increments 1째C

Up and down agitation : Delay time setting:

4 times every 5 min. 99h 99 min.

UPS:

Outside

Nominal supply voltage: Maximum power draw:

220 V / 50 Hz 110 V / 60 Hz 1000 VA

Dimension: Width: Depth: Height: Weight:

1400 mm 500 mm 550 mm 142 Kg

Instruments

Number of Stations: Number of Paraffin Stations Number of stored programs: Number of samples in process: Capacity of single tray:

35


Histo-Pro 200

Vacuum Tissue Processor Extremely Reliable

Technical Data: Nominal voltage: AC220V Nominal frequency: 60HZ Main fuses: 10A Nominal power: 1000 VA Dry weight, unpacked: approx. 100 kg Weight, packed: 120 kg Operating temperature range: 15 °C to 35 °C Relative humidity: 10 to 80 %, Paraffin wax baths Number of baths: 3 Volume (liters): 4.0 l per bath Melt time: approx. 3 hrs Temperature: 65 Temperature accuracy: + 1 Retort Capacity: approx. 200 cassettes Reagent volume : 5.3 Lt. Temperature (paraffin): 65 °C Temperature (reagents): ambient or 35°C Temperature (cleaning reagents): 65 °C Temperature accuracy: + 1 °C Filling time: 5 min Drain time: 5 min Impregnation vacuum: -70 kPa (g) Impregnation pressure: 35 kPa (g) Fill vacuum: -70 kPa (g) Drain pressure: 35 kPa (g)

General Reagent bottles: 9 Cleaning solution bottles: 3 Maximum bottle volume: 4.0 l Recirculation (pump in/out): ON/OFF Time between cycles: 15 min Hardware and Software: Large color Liquid Crystal Display (LCD) User-friendly, intelligent software. Alarm system to reminder wrong operation and troubles Multiple specimen protection system. Capacities: 15 programs that consist of up to 9 reagent and 3 paraffin wax steps. Time per program step: 0 to 99 hours, 59 minutes. Up to 200 cassettes can be processed Wax clean program. 9 reagent bottles. 3 wax baths. 3 cleaning solution bottles. 2 condensate bottle.

Instruments 36


SA3000 Series

Biological Microscope

Specifications: Magnification Objective / eyepiece

4X

10X

40X

100X (oil)

wide field high-eye 10X

40X

100X

400X

1000X

64X

160X

640X

1600X

point

WF 16X

Mechanical stage size of stage: 142mmX 132mm moving range: 55mm X 75mm Ocular head Monocular: incline 45° rotational 360° Binocular: incline 45° rotational 360° Dual View: incline 30° rotational 360° Trinocular: incline 30° rotational 360° Power supply (1) universal input voltage: 100V-240V (2) output voltage: 6V-20W (3) fuse: 1.5A/ Ø5 x 20 item ocular head

SA3100

binocular incline 45 °

Monocular incline 45°

SA3200 dual view incline 30°

SA3300 Trinocular incline 30°

rotational 360° eyepiece

wide field view and high-eye point eyepiece 10X/18, P 16X

objective

Achromatic objective 4X, 10X, 40X, 100X (oil)

stage coarse/fine focusing system condenser illumination system

double mechanical stage: 142mmX 132mm, moving range: 55mm x 75mm coaxial coarse and fine system adjustment arrange 0.002mm20mm

Instruments

SA3000

Kohler type, condenser with variable diaphragm, the adjustment of rising and falling build-in halogen light: 6V/20W

37


Cito-Spin

Cytology Centrifuge

Features : Cytological centrifuge is controlled by microprocessor Program save and recall up to ten protocols Gentle stop and fast braking : pre select 10 braking rates Warning indicators for imbalance, over speed, door open, setting failure. Rotor imbalance is detected and immediately signaled to operator

Instruments

Specification: Max RPM : Max RCF : Maximum Capacity : Timer : Braking rates: Programmability: Digital display : Drive Motor : Power : Dimension:3 Specimen range: Weight:

3,000 rpm 1,057 x g 12 Slide x 6cc 99min 59sec or "free" for long run 10 setting 10 memory Speed, Time, Brake, Program High torque DC Motor 220V, 50/60Hz, Single Phase, 0.5Kw C. 60 W x 410 D x 280 H mm. 0,5 to 6 cc. 24 Kg.

Accessories: Sample clip angle Rotor : Max RPM : Max Capacity : Cell : Rubber plate : Cell Holder :

1 set 3,000 rpm Max RCF : 1,057 x g 12 Slide x 6cc 6cc (for 14x14mm) 12ea/set 6cc (for 14x14mm) 12ea/set 12ea/set

1set 1set 1set

38


39


Distributed by:

Histo Line Laboratories S.r.l. Via Brembo,27-20139 Milano ITALY. Tel:+39 0255230061 Fax:+39 0255213764 Email: histoline@histoline.com URL: www.histo-online.com - www.histoline.com

Histo-Line Histology Catalog 2010  

Fixation / Processing / Embedding / Sectioning / Staining / Storage / Instruments

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