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Biomed Biotechnol. 2010; 2010:283842. Epub 2009 Dec 13. PubMed PMID: 20037731; PubMed Central PMCID: PMC2796335.

3: Manning-Cela R, S Jaishankar, J. Swindle Life-cycle and growth-phase-dependent Dra. Rebeca Georgina Manning Cela rmanning@cinvestav.mx

regulation of the ubiquitin genes of Trypanosoma cruzi. Arch Med Res 2006 July, 37 (5) :593-601. PubMed PMID: 16740428.

Investigator 3C Category in the SNI: Level I Doctor of Science in Cell Biology (1994), CINVESTAV-IPN.

Gallery

Phone: +52 (55) 5747-5010 Fax: +52 (55) 5747-3938 rmanning@mail.cinvestav.mx

EGFP

FALOIDINA

DAPI k n

EMPALME1

k n

EMPALME2

 Studies of molecular and cellular mechanisms of the infection process of Trypanosoma cruzi.

Representative publications:

1: Florencio-Martinez L, Marquez-Dueñas C, Ballesteros-surrounds G, Martinez-Calvillo S, Manning-Cela R. Cellular host cell analysis of Infection by Different developmental stages of Trypanosoma cruzi. Exp Parasitol. 2010 Nov, 126 (3) :332-6. Epub 2010 Apr 28. PubMed PMID: 20433833.

2: Hernandez-Osorio LA, Marquez-Dueñas C, Florencio Martinez-LE, Ballesteros-surrounds G, Martinez-Calvillo S, Manning-Cela RG. Improved method for in vitro secondary amastigogenesis of Trypanosoma cruzi: morphometrical and Molecular analysis of intermediate developmental forms. J Centro de Investigación y de Estudios Avanzados del IPN

Figura. Epimastigotes de Trypanosoma cruzi invadiendo fibroblastos 3T3 NIH . Observación directa de epimastigotes Knock-in expresando la proteína verde fluorescente (EGFP) analizados por microscopía confocal. Las muestras se tiñeron con faloidina rodaminada y se contratiñeron con DAPI para visualizar el citoesqueleto de actina (FALOIDINA) y el DNA (DAPI) del núcleo (n) y cinetoplasto (k) respectivamente. Se muestra el empalme de las imágenes de EGFP, FALOIDINA y DAPI (EMPALME1) o EGFP y FALOIDINA (EMPALME2). Las flechas indican la huella del parásito en el citoesqueleto de actina de la célula hospedero y la huella de las fibras de actina en el flagelo del parásito demostrando que los epimastigotes son capaces de invadir la célula blanco. Las cabezas de flecha indican fibras de actina de la célula hospedero rodeando el flagelo del parásito. Las líneas punteadas muestran la amplificación del parasito señalado. Magnificación 63X


Education Degree: Doctorate of Science in the specialty of Cell Biology Department of Cell Biology Research Center and Advanced Studies of IPN. Mexico, DF, Mexico. Postdoctoral: Seattle Biomedical Research Institute. Seattle, WA, USA and Department of Pathobiology University of Washington. Seattle, WA, USA. SNI level.

SNI I A. - Research Line (updated to 2011), citing existing collaborations with other institutions.

1) Study of the molecular mechanisms involved in the process of infection and stage of differentiation of Trypanosoma cruzi. 2) Identification and characterization of molecules important for the infection process and stage of differentiation of Trypanosoma cruzi. 3) Analysis of the involvement of the actin cytoskeleton of the host cell during infection of T. cruzi. 4) Analysis of the specific role of β-actin isoform in 3T3 fibroblasts. 5) Epidemiological, entomological, parasitological and molecular T. cruzi in Mexico. 2. - Brief curriculum vitae (updated).   Publications in refereed journals: 20 Publications at length: 9 Book Chapters: 2

  In my laboratory we are interested in studying the molecular and cellular mechanisms of infectious diseases, particularly those biological processes that are important to allow the Trypanosoma cruzi infects human efficiently. This parasite is the etiologic agent of Chagas disease, a condition that even when it was discovered over a century ago remains an incurable disease, highly disabling and generates high costs of treatment and a very high cost to the health sector. It ranks first among tropical diseases and the fourth among diseases, representing a loss of 0.667 million years of disability-adjusted life, which corresponds to the figure of greatest impact on Latin America and places it as the parasitic infection more important in endemic countries in the Americas. T. cruzi is an obligate intracellular parasite that infects a wide variety of cells through a complex infection process involves the participation of various molecules of both the host cell the parasite, but very few have been characterized to date. Thus our studies have focused on the identification and characterization of molecules involved in the infection process and differentiation stage T. cruzi, using molecular and cellular approach through the preparation and characterization of subtraction libraries and parasite cells and knock-out, knockin and dominant negative mutants. In this way we aim to identify those molecules that have a role in these processes and therefore the survival of the parasite, could be potential targets for developing vaccines and drugs for disease control. We also study the process of invasion of different developmental stages of T. cruzi using parasites expressing knock-in sequence encoding the green protein (EGFP) or red fluorescent (DsRed) for in vivo studies. Using this approach, we also studied the role of the actin cytoskeleton of the host cell is in the process of infection of T. cruzi. For this we obtained and characterized target cells expressing a b-actin sequence fused to the sequence encoding the green fluorescent protein (EGFP) as well as variants in which actin mutated sequence to obtain different dominant negative mutants of b -actin. These mutants not only allowed us to study the role of the actin cytoskeleton of the target cell during the infection process of T. cruzi, but also the specific function of b-actin isoform. Finally, as part of a network of Chagas involving the participation of different groups in different states of the country and abroad, we conducted epidemiological, entomological, parasitological and molecular, using GIS and spatial modeling to analyze data and establish correlations that allow the development of predictive spatio-temporal analysis of the behavior of the disease transmission risk. These studies provide useful information in the future could be used for establishing monitoring programs and disease control.

Participation in Congresses: 84 Graduate students: 10 Bachelor, Masters and 2 Doctoral 9 Students in the process: 1 Masters and 5 PhD Courses taught: 33 Coordinated courses: 11

3. - Brief description of work done in the laboratory, emphasizing the techniques used.

Centro de Investigación y de Estudios Avanzados del IPN

4. - Brief description of recent results, including photos, illustrations and diagrams attractive (for purposes of disclosure).


Colaboraciones

Institución

Dr. Santiago Martinez Cavillo Dr. Roberto Hernández Fernández y Dra. Ana María Cevallos Gaos Dr. Ricardo Mondragón Flores

UBIMED, FES-Iztacala, UNAM

Institutoe of Biomédicas, UNAM Departament of Bioquímica, CINVESTAVIPN Dr. Luis G. Brieba de Castro CINVESTAV Unidad Irapuato Dr. Pedro Antonio Reyes López Institute National of Cardiología Ignacio Chávez Formo parte y colaboro con diferentes integrantes de la Red de Chagas Dr. Juan Carlos Vázquez Chagoyán y Dr. AleUAEM jandro De Las Heras Dra. Ma. del Carmen Guzmán Bracho INDRE Dra. Rebeca G. Manning Cela Departamento de Biomedicina Molecular. CINVESTAV-IPN Dra. Nisha Jain Garg Microbiology and Immunology and Pathology Departments. University of Texas Medical Branch. Galveston Texas. Dr. Jose Guillermo Estrada Franco Pathology Department, University of Texas Medical Branch. Galveston Texas y CEMESAD UNACH Tapachula, Chiapas Dr. José Antonio de Diego Cabrera U. Parasitología y Medicina Tropical-Depto,. Medicina Preventiva y Salud Pública. Fac. Medicina-Universidad Autónoma de Madrid (U.A.M.). Dra. Ma. Elena Villagrán Herrera Depto. Investigación Biomédica-Fac. Medicina. Univ .Autónoma Qro. Dr. Eleuterio Campos Hernández y Dra. Jose- Instituto de Investigación Científica Área fina Munguía Aldama Ciencias Naturales, Universidad Autónoma de Guerrero Dra. Eugenia Guzmán Marín, Dra. Karla Y. Centro de Investigaciones Regionales “Dr. Acosta Viana y Dra. Matilde Jiménez Coello Hideyo Noguchi” Universidad Autónoma de Yucatán Dr. José Luis Imbert Palafox Universidad Autónoma de Hidalgo

Centro de Investigación y de Estudios Avanzados del IPN


Centro de Investigaci贸n y de Estudios Avanzados del IPN


Centro de Investigaci贸n y de Estudios Avanzados del IPN


11. Cell Microbiol. 2003 Dec, 5 (12) :921-32.

5. - Recent representative publications (where possible, including pdf file of the article). 1. Trypanosoma cruzi: multiple actin isovariants are Different developmental stages Observed along. Cevallos AM, Segura-Kato YX, Merchant-Larios H, Manning-Cela R, Alberto Hernandez-Osorio L, Marquez-Dueñas C, JR Ambrose, Reynoso-Ducoing O, Hernández R. Exp Parasitol. 2011 Jan, 127 (1) :249-59. Epub 2010 Aug 9. PMID: 20705070 2. Cellular analysis of host cell by Different developmental stages Infection of Trypanosoma cruzi. FlorencioMartinez L, Marquez-Dueñas C, Ballesteros-surrounds G, Martinez-Calvillo S, Manning-Cela R. Exp Parasitol. 2010 Nov, 126 (3) :332-6. Epub 2010 Apr 28. PMID: 20433833

12. PMID: 14641177 13. Signal transduction in Entamoeba histolytica induced by interaction with fibronectin: Presence and activation of phosphokinase A and Its possible relation to invasiveness. 14. Franco E, Manning-Cela R, Meza I. 15. Arch Med Res 2002 Jul-Aug, 33 (4) :389-97. 16. PMID: 12234529 17. Alternative splicing of LYT1 transcripts in Trypanosoma cruzi. Manning-Cela R, González A, Swindle J. Infect Immun. 2002 Aug;70(8):4726-8. PMID: 12117992

3. Gene expression in trypanosomatid parasites. Martinez-Calvillo S, Vizuet de Rueda JC, Florencio-Martinez LE, Manning-Cela RG, Freeman EE-Angulo. J Biomed Biotechnol. 2010; 2010:525241. Epub 2010 Feb 11. Review. PMID: 20169133

18. Secretory pathway activation by interaction of Entamoeba histolytica trophozoites with fibronectin. Manning-Cela R, Carbajal EM, Meza I. Arch Med Res. 2000 Jul-Aug;31(4 Suppl):S153-4. No abstract available. PMID: 11070263

4. Improved method for in vitro secondary amastigogenesis of Trypanosoma cruzi: morphometrical and molecular analysis of intermediate developmental forms. Hernandez-Osorio LA, Marquez-Dueñas C, FlorencioMartinez LE, Ballesteros-surrounds G, Martinez-Calvillo S, Manning-Cela RG. J Biomed Biotechnol. 2010; 2010:283842. Epub 2009 Dec 13.PMID: 20037731

19. LYT1 protein is required for efficient in vitro infection by Trypanosoma cruzi. Manning-Cela R, Cortés A, González-Rey E, Van Voorhis WC, Swindle J, González A. Infect Immun. 2001 Jun;69(6):3916-23. PMID: 11349059

5. Gene organization and sequence of transfer RNA genes Analyses in Trypanosomatid parasites. PadillaMejia NE, Florencio-Martinez LE, Figueroa-angle EE, Manning-Cela GR, Hernandez-Rivas R, Myler PJ, Martinez-Calvillo S. BMC Genomics. 2009 May 18; 10:232. PMID: 19450263 6. EspF Interacts with nucleation-promoting Factors to recruit junctional proteins pedestals for pedestal Into Maturation and disruption of paracellular permeability. Peralta-Ramirez J, Hernandez JM, Manning-Cela R, Luna-Muñoz J, Garcia-Tovar C, Nougayréde JP, Oswald E, Navarro-Garcia F. Infect Immun. 2008 Sep, 76 (9) :3854-68. Epub 2008 Jun 16. PMID: 18559425 7. Life-cycle and growth-phase-dependent regulation of the ubiquitin genes of Trypanosoma cruzi. ManningCela R, S Jaishankar, J. Swindle Arch Med Res 2006 Jul; 37 (5) :593-601. PMID: 16740428 8. Increased angiotensin II-dependent expression of Na +-glucose cotransporter in hypertension. Bautista R, Manning R, Martinez F, Avila-Casado mdel C, Soto V, Medina A, Escalante B. Am J Physiol Renal Physiol. 2004 Jan, 286 (1): F127-33. Epub 2003 Sep 23. PMID: 14506074 9. BFA-sensitive and insensitive exocytic pathways in Entamoeba histolytica trophozoites: their relationship to pathogenesis. 10. Manning-Cela R, Marquez C, Franco E, Talamas-Rohana P, Meza I. Centro de Investigación y de Estudios Avanzados del IPN

20. Signal transduction pathways in Entamoeba histolytica: PKA activity and translocation during the interaction of trophozoites with FN. Franco E, Manning-Cela R, Meza I. Arch Med Res. 2000 Jul-Aug;31(4 Suppl):S126-7. PMID: 11070252 21. Up-regulation of action mRNA and reorganization of the cytoskeleton in Entamoeba histolytica trophozoites. Manning-Cela R, Meza I. J Eukaryot Microbiol. 1997 Jan-Feb;44(1):18-24. PMID: 9172829 22. cAMP levels and up-regulation of actin mRNA in Entamoeba histolytica. Manning-Cela R, Piña A, Meza I. Arch Med Res. 1997;28 Spec No:134-5. No abstract available. PMID: 9033042 23. Fibronectin-induced intracellular calcium rise in Entamoeba histolytica trophozoites: effect on adhesion and the actin cytoskeleton. Carbajal ME, ManningCela R, Pina A, Franco E, Meza I. Exp Parasitol. 1996 Jan;82(1):11-20.PMID: 8617326 24. Actin mRNA levels and actin synthesis during the encystation of Entamoeba invadens. Manning-Cela R, Meraz MA, Hernandez JM, Meza I. J Eukaryot Microbiol. 1994 Jul-Aug;41(4):360-5. PMID: 8087106 25. Heterogeneity of the ribosomal DNA episome in strains and species of Entamoeba. Cázares F, Manning-Cela R, Meza I. Mol Microbiol. 1994 May;12(4):607-12. PMID: 7934884


6. - Working current (if you want you can include emails). Graduates with attachment sites when they have.   Cargo

Nombre

Correo electrónico

research assistant in

Q.F.B. Claudia Marquez Dueñas

cmarquez@cinvestav.mx

Posdoctorado

Dr. Gilberto Ballesteros Rodea

gibaro90@hotmail.com

Estudent of Doctorated

M. en C. César Israel Lugo Caballero

cesarl@mexico.com

Estudent of Doctorated

M. en C. Daniel Sánchez Cruz angel_de_luz_666_7@hotmail. com

Estudent of Doctorated

M. en C. Luis Alberto Hernandez Osorio

luisheol@hotmail.com

Estudent of Doctorated

M. en C. Carla Angulo Rojo

carlaibq@yahoo.com.mx

Estudent of Doctorated

M. en C. Julio Elias Alvarado Yaah

alvaram25@hotmail.com

Tesista de Licenciatura

Teresa Handehui Martínez Cuevas

martinez.t7@gmail.com

Tesista de Licenciatura

Alberto Antonio Campos

albertoantonio86@gmail.com

Centro de Investigación y de Estudios Avanzados del IPN


Dr.Rebeca