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Datasheet of Spautin 1
Description: Spautin-1 is a novel autophagy inhibitor, IM inhibited the growth of K562 cells with IC50 of 1.03 μM. In contrast, co-treatment with Spautin-1 increased IM-induced inhibition of cell viability with IC50 of 0.45 μM. in vitro: A novel autophagy inhibitor named specific and potent autophagy inhibitor-1 (Spautin-1), which markedly inhibits Imatinib mesylate (IM)-induced autophagy in chronic myeloid leukemia (CML) cells by downregulating Beclin-1. Spautin-1 markedly attenuated the protein levels of Beclin-1 induced by IM, but had no significant effect on other autophagy factors. Spautin-1 enhances IM-induced apoptosis by inactivating PI3K/AKT and activating downstream GSK3β, leading to downregulation of anti-apoptotic protein Mcl-1 and Bcl-2, which represents a promising approach to improve the efficacy of IM in the treatment of patients with CML.IM inhibited the growth of K562 cells with IC50 of 1.03μM at 48 h. In contrast, co-treatment with Spautin-1 increased IM-induced inhibition of cell viability with IC50 of 0.45 μM.Spautin-1 inhibits IM-induced autophagy, enhances IM-induced cytotoxicity and apoptosis in K562 cells, decreasing the IC50 from 1 to 0.5 μM. in vivo: Spautin-1 inhibits starvation-induced autophagy in solid tumor cells.
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Store at -20°C (desiccating conditions).
Handling: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months. Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room
temperature for at least 1 hour.