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Biomedical Applications Forthcoming


Optical Techniques in Regenerative Medicine

Second Harmonic Generation Imaging

Edited by

Edited by

Stephen P. Morgan

Francesco S. Pavone

University of Nottingham, UK

Universita degli Studi di Firenze, Sesto Fiorentino, Italy

Felicity R.A.J. Rose University of Nottingham, UK

Stephen J. Matcher

Paul J. Campagnola University of Connecticut, Farmington, USA

University of Sheffield, UK

Series in Cellular and Clinical Imaging

This book is focused on the optical techniques that can be applied to regenerative medicine, including optical coherence tomography, acousto-optic imaging, Raman spectroscopy, machine vision, polarized light imaging, fiber optic sensors, second harmonic generation, multi-photon microscopy, coherent anti-Stokes Raman scattering, and polarized light imaging. It covers applications both in fundamental research and in the regenerative medicine industry for tissue engineering products. Each chapter gives an overview of a particular technique, its advantages and limitations in terms of structural and functional information provided, and examples of applications in regenerative medicine. The book provides a practical guide to the most appropriate techniques for a given application. It also offers a summary of major recent advances, such as use of machine vision for tracking growth of threedimensional constructs in bioreactors and polarization sensitive optical coherence tomography for monitoring collagen alignment in cartilage growth.

“... provides up-to-date coverage of our current understanding of harmonic imaging principles and also the most important biomedical applications of this technique.”

• Gives a detailed background to the most important optical techniques used in regenerative medicine, including basic information and the latest developments • Addresses needs of the biomedical/tissue engineering industry by focusing on applied areas, such as development of new products— biological substitutes that will restore, maintain, or improve tissue function • Covers techniques for monitoring the process of three-dimensional tissue culture both in basic research and as a tool for manufacturing new regenerative medicine products • Discusses the advantages and limitations of using particular techniques in the context of various applications Catalog no. K12560, August 2013, c. 488 pp. ISBN: 978-1-4398-5495-2, $129.95 / £82.00 Also available as an eBook

—From the Foreword by Peter T.C. So, Harvard University

This book provides a complete overview of the principles underlying this technique and its current applications, including use in molecular sensing. • Focuses on building a system for second harmonic generation imaging, choice of external labels, current research, and clinical uses • Provides a tutorial on the subject as well as an up-to-date review of state-of-the-art applications • Gives an overview of principles underlying second and third harmonic generation imaging • Covers chromophores, biomedical imaging aspects, use of SHG for order and structure studies, imaging of micro tubules, and other subcellular components

Selected Contents Principles Of Shg Imaging: Second Harmonic Generation: Theory. How to Build Up an SHG Apparatus. THG Microscopy of Cells and Tissues: Contrast Mechanisms and Applications. Comparison of SHG, THG, CARS, and Multiphoton Excited Fluorescence Imaging. Biomedical Imaging Using SHG: Molecular Structure and Order with Second Harmonic Generation Microscopy. 3D SHG Imaging and Analysis of Fibrillar Collagen Organization. Second Harmonic Generation Imaging of Microtubules. SHG and Optical Clearing. Holographic SHG Imaging. Imaging Membrane Potential with SHG. Applications of SHC: SHC of SelfAssembled Gels. SHG Imaging of the Eye. SHC Imaging of Cardiovascular Disease. Combined SHC/THC Imaging. SHG Imaging of Collagen and Application to Fibrosis Quantization. SHG Imaging of Breast Cancer. Second Harmonic Generation in Tumors: Scattering and Polarization. SHG Imaging for Tissue Engineering Applications. Catalog no. K12222, July 2013, c. 576 pp. ISBN: 978-1-4398-4914-9, $149.95 / £95.00 Also available as an eBook

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