a product message image
{' '} {' '}
Limited time offer
SAVE % on your upgrade

Page 1

Oncolytic Virus Efficacy Study The currently approved oncolytic virus drugs and different clinical trials have proved promising efficacy data. Preclinical study of in vivo efficacy will provide more powerful information for later stage development, including optimization of administration route, dose, and clinical trial design. In vivo efficacy studies include but are not limited to the following categories. •

Antitumor effect of oncolytic virus The antitumor effect of oncolytic virus can be analyzed by calculating the tumor size, tumor weight or through in vivo imaging, such as bioluminescence imaging (BLI) and position emission tomography (PET).

D Kuruppu, AL Brownell, K Shah, U Mahmood, KK Tanabe. 2014. Molecular imaging with bioluminescence and PET reveals viral oncolysis kinetics and tumor viability. Cancer Res. 2014 Aug 1; 74(15): 4111–4121 •

Transgene expression and transgene function in vivo In order to further demonstrate the efficacy of oncolytic virus, the transgene engineered into oncolytic viruses will be analyzed for its expression and function in vivo. Molecular biology (ELISA, western blot, qRT-PCR, etc.) and cell biology (cell culture, IHC, etc.) techniques will be applied to the analysis.

Immunological analysis As most oncolytic viruses incorporate immune factors (antibody, cytokine, growth factor, etc.) into the tumor treatment, immunological analysis should be applied in the


efficacy, including flow cytometry analysis, Elispot, T cell proliferation, activation, exhaustion and memory phenotype, chemotaxis, etc. •

Histological and pathological analysis Histological and pathological analysis are not only adopted to study the toxicity of oncolytic virus, but also for the efficacy studies. The changes in a tumor, tumor stroma, or immune cell infiltration can be concluded from the histological and pathological analysis.

Clinical observation Characteristics, covering feed consumption, body weight, behavior, and morphology are closely monitored. After specific end points, a complete necropsy including the assessment of organ weight and histopathology analysis is conducted. The survival data is also recorded and statistically analyzed properly.

Reference: 1. D Kuruppu, AL Brownell, K Shah, U Mahmood, KK Tanabe. (2014). “Molecular imaging with bioluminescence and PET reveals viral oncolysis kinetics and tumor viability.” Cancer Res. 74(15), 4111–4121.

Profile for Candy Swift

Oncolytic Virus Efficacy Study  

Preclinical study of in vivo efficacy will provide more powerful information for later stage development, including optimization of administ...

Oncolytic Virus Efficacy Study  

Preclinical study of in vivo efficacy will provide more powerful information for later stage development, including optimization of administ...

Advertisement