Instructions: Use the videos on graphing kinetics data in Google Docs, Apache OpenOffice, and Microsoft Excel to help you answer the assessment question below. Note that a sample calculation is available in the next component. If the question gives you trouble, consider peeking ahead to see one way to approach this problem. Learning Goals: To learn how to graph kinetics data and use the resulting plot to understand the activity of an enzyme.
Linest in Google Docs spreadsheet video Please watch the online video (3 minutes 43 seconds).
Linest in OpenOffice spreadsheet video Please watch the online video (4 minutes 9 seconds).
Linest in Microsoft Excel video Please watch the online video (3 minutes 45 seconds). Please complete the online exercise. OPTIONAL‐Please participate in the online discussion forum.
Sample calculation ‐ Lineweaver‐Burk plot Background: Lineweaver‐Burk plots depict 1/V vs. 1/[S]. These are very useful for determining the nature of enzyme‐substrate interactions.
Instructions: Review the sample calculation demonstrating the use of V‐[S] data points to generate a Lineweaver‐Burk plot. Learning Goal: To learn how to use V‐[S] data points to determine Vmax and Km of an enzyme‐ substrate system. Task: Determine Vmax and Km based on the provided data points. V (mmol/min)