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Le 18/04/2019

Confidentiel - King Tree - 2018


King Tree 1989 : Jean-BenoĂŽt Tierny starts developing the use of Chestnut Wood Extract in animal feed with ECOPSI*

Since 2016, Jean-BenoĂŽt and Pierre-Hugues Tierny relaunch with King Tree Chestnut Wood extraction in France for animal and human Nutrition only

*ECOPSI : Etude et COmmercialisation de Produits Secondaires Industriels

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King Tree

Why King Tree ? Why Chestnut Tree? Chestnut tree is the King Tree in France : it feeds, it heats, it’s a construction material and now it heals! Chestnut Tree naturaly produces hydrolysable tannins, more specifically ellagitannins

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Our unique factory King Tree built in France a New Factory which has been operational since early 2018. Our factory is completely automated and unique in the world We only use French Chestnut Trees to produce our only product : The Kingbrown® Our production is FCA Certified = GMP+ (Feed Chain Alliance)

We are defending a strong « Cradle to Cradle » Philosophy Environmental friendly & ethical philosophy No pollution & No production waste |4


Our unique factory

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Our Cradle to Cradle Philosophy

Reuse of the water from the pressing process and the condensates Natural growth of chestnut trees in 20 years

Manufacture of fibre wood panels thanks to the wood chips from the process = NO WASTE

La Tarnaise des Panneaux

King Tree Factory Use of french biomass in the immediate vicinity of the factory

(Labruguière, Tarn) Water extraction of molecules of interest without any effluent discharge

Wood natural water content is used for the extraction

Kingbrown® Chestnut wood extract

100 % French – 100 % Natural – 100 % from Chestnut Trees Rich in molecules of high interest for Nutrition and Health

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KingbrownÂŽ

100% chestnut Extract, 100 % French & 100 % natural

Authorized as an additive in Europe in animal feed Feed Chain Alliance Certification

Organic certification Authorized as food additive and natural flavouring (human nutrition) ISO 22000 certification underway |7


KingbrownÂŽ Tannins = polyphenolic biomolecules

Hydrolysable tannins Gallotanins

Ellagitannins

Condensed Tannins Quebracho Mimosa

Grape

Vast Majority of polyphenolic biomolecules = condensed tannins

Gallnuts Pomegranate

Chestnut Tree

The KingbrownÂŽ is a very specific and unique product. It is rich in specific polyphenols : the ellagitannins. The specific structure of the ellagitannins from french chestnut give unique properties to the KingbrownÂŽ, which differ from the vast majority of tannins (condensed tannins) |8


Kingbrown® : properties

Characteristics of Kingbrown® : Very powerful and stable Antioxydant Chemical bonding with proteins and metals (Iron) With the recommended doses (from King Tree), no reaction with : amino-acids, peptides, casein enzymes (pepsines, trypsines, phytases…) Reinforces the intestinal epithelium by enhancing the gut barrier thanks to several mechanisms*

Preventive Action on the intestinal health Without decreasing the zootechnical performances Lenzi et al., BMC Compl. and Alt. Med. (2017) 17:251 / Viladomiu et al., Evidence-Based Compl. and Alt. Med. (2013) 789764 / * Confidential studies

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Application in Animal Nutrition and Health

Health overall objectives : Preventive Protection against many intestinal diseases of bacterial, viral & parasitic origin thanks to the overall strengthening of the gut barrier Positive orientation of gut microbiota : Limits the amount of proteolytic flora (Clostridium) , boosts the growth of bacteria such as Lactobacillus, Bifidobacterium, Akkermansia Protection against diarrhea (calves or piglets) thanks to mecanical action of KingbrownÂŽ on the intestinal mucosa

KingbrownÂŽ is an efficient substitute to agressive treatments (antibiotics, coccidiostat, essential oils)

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Specificy per species : Poultry

Health objective : Preventive protection against coccidiosa Allows to substitute totally « aggressive » treatments: antibiotics, anticoccidials (including vaccines), essential oils… Without modifying zootechnical performances

Without modifying the coloration

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Application in a label broilers breeding

Comparison Kingbrown vs Maxiban in label white broilers (JA 657) : Trial in 2 buildings of 400 m², which means 2 x 4400 broilers per site: Control building: 90 ppm of Maxiban from 0 to 60 days Trial building:

2 kg Kingbrown/ ton of feed from 0 to 21 days

1 kg Kingbrown / ton of feed from 22 to 60 days

The 2 batches were managed identically (same food presentation, same temperature…) to study the only efficiency of Kingbrown |12


Application in a label broilers breeding

Results : Reid Index (31 to 35 days): E. Acervelina Average Maxiban batches average 0.59 Kingbrown batches average 0.3

E. Tenella Average 0.07 0.12

Technico-economic results: 27 g decrease of the average weight on Kingbrown batches IC increase of 0,02 on Kingbrown batches Feed cost: Decrease of 80â‚Ź / Kingbrown batch

The decrease of weight and IC is balanced by a decrease of feed cost

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Application in a label broilers breeding

Assessment of the trail: Good management of coccidiosis with KingbrownÂŽ Economically viable solution to replace synthetic anticoccidials Generalization of Kingbrown on the white and yellow label productions of the organization since the beginning of 2018

Average results of 2018 are identical to 2017 (out of 2 million chickens label)

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Application in experimental breeding Evaluation of Kingbrown effects on label broilers infected by Eimeria or Salmonella + Eimeria

TS : Kingbrown + Salmonella + Coccidiosis ; CS : Control + Salmonella + Coccidiosis ; TC : Kingbrown + Coccidiosis ; CC : Control + Coccidiosis

Even in the presence of Salmonella, KingbrownÂŽ can effectively reduce excretion of oocysts in the faeces

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Application in poultry Bacterial translocation Passage of digestive origin bacteria through the intestinal mucosa to extra-intestinal sites Reducing bacterial translocation helps to strengthen the barrier-effect of the intestinal wall and protect the body against many pathogens

King Tree has conducted in vivo studies* to investigate the effect of Kingbrown on bacterial translocation

Objectives of the study: to measure the passage of bacteria from the intestine to the spleen (reflection of the blood circulation), mesenteric adipose tissue (MAT) and mesenteric lymph ndoes (MLN).

*Studies on a specific model of infection by Citrobacter rodentium, model used to mimic Escherichia coli infection.

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Animal où il y a eu translocation bactérienne vers le site identifié (%)

Application in poultry 86

The incorporation of Kingbrown® into the feed ration of the animals has led to a marked decrease in bacterial translocations from the intestine to the spleen (almost 67% decrease in translocation

40 28*p<0.01

33 21

compared to an infected animal), mesenteric adipose tissue (MAT), and mesenteric lymph nodes

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(MLN). MAT Animal infecté par Citrobacter

MLN

RATE

Animal Infecté par Citrobacter + Kingbrown®

Protective effect of Kingbrown that helps strengthen the intestinal barrier to protect the body from pathogens |17


Application in poultry Strengthening of the intestinal barrier, including reduction of bacterial

translocation Reduced availability of high molecular weight proteins for bacterial fermentation Reduction of intestinal origin pathologies: infection with E. coli and

Clostridium perfringens |18


SAS KING TREE - 15 bis Rue des Grands Viéziers - 62 000 ARRAS - Tel. : + 33 (0)3 21 60 32 70 - Mail : contact@kingtree.eu

www.kingtree.eu

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