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The Gram Stain

1. Add Crystal Violet for 1 minute

Rinse with Water

2. Add Iodine for 1 minute

Rinse with Water 3. Decolorize with alcohol solution until no color comes off (2-5 seconds)

Rinse with Water

4. Counterstain with safranin for 30 seconds

Rinse with Water 5. Air dry smear and observe under the microscope


Gram Stain Quick Reference