Enzyme Large-scale Production

Industrial Enzyme Production

Discover more at creative-enzymes.com

1

Overview Methods of enzymes production Extraction and separation Chemical synthesis Biosynthesis Microbial fermentation Types of microorganism Types of microbial fermentation Process of microbial fermentation

Discover more at creative-enzymes.com

2

Methods of enzymes production

Extraction and separation Enzymes production

Chemical synthesis Biosynthesis

Discover more at creative-enzymes.com

3

Methods of enzymes production

1. Extraction and separation Animal tissue

Plant tissue

Grinding

Extraction

Filtration

Concentration

Purification Discover more at creative-enzymes.com

4

Methods of enzymes production

2. Chemical synthesis

A method for synthesizing a novel catalyst with high efficiency, high selectivity, simple structure and high stability by chemically simulating the active center and catalytic mechanism of the enzyme.

Discover more at creative-enzymes.com

5

Methods of enzymes production

3. Biosynthesis—Microbial fermentation

The required enzymes are produced by microbial culture under controlled conditions. Microorganisms are the most significant and convenient sources of commercial enzymes. They can be made to produce abundant quantities of enzymes under suitable growth conditions.

Discover more at creative-enzymes.com

6

Overview Methods of enzymes production Extraction and separation Chemical synthesis Biosynthesis Microbial fermentation Types of microorganism Types of microbial fermentation Process of microbial fermentation

Discover more at creative-enzymes.com

7

Microbial fermentation

1. Types of microorganism

Fungi

Yeast

Discover more at creative-enzymes.com

Bacteria

Actinomycetes

8

Microbial fermentation

1. Types of microorganism Microorganisms

Types

Aspergillus oryzae

Fungi

Saccharomyces

Yeast

cerevisiae Bacillus subtilis

Bacteria

Streptomyces sp.

Actinomycetes

Discover more at creative-enzymes.com

Enzymes Amylases, Cellulases, Lipases Amylases, Invertase Cellulases, Pectinases, Phytases, Proteases Amylases, Isomerase

9

Microbial fermentation

2. Types of microbial fermentation

Submerged fermentation Microbial fermentation Solid-state fermentation

Discover more at creative-enzymes.com

10

Microbial fermentation

2. Types of microbial fermentation——Submerged fermentation Acid/base Antifoam

Inlet Air Flow

Exit Gas Flow Fresh Media Feed

Level Sensor

Agitator Sparser

pH Sensor Dissolve O2 Sensor Thermocouple

Exit Liquid Flow

Discover more at creative-enzymes.com

11

Microbial fermentation

2. Types of microbial fermentation——Solid-state fermentation

Discover more at creative-enzymes.com

12

Microbial fermentation 3. Process of microbial fermentation Microbial culture

Fermentation

Propagation

Separation

Extracellular products (supernatant)

Intracellular products (cell biomass)

Liquid enzyme concentrate

Separation (liquid/solid)

Precipitation

Precipitation

Filter press

Purification & Stabilization

Vacuum or spray drying

Drying

Packaging

Packaging

Discover more at creative-enzymes.com

13

Industrial enzyme production service

Industrial enzyme production service——Creative Enzymes

Discover more at creative-enzymes.com

14

Industrial enzyme production service

Industrial enzyme production service——Creative Enzymes

Discover more at creative-enzymes.com

15

Industrial enzyme production service

Industrial enzyme production service——Creative Enzymes

Email contact@creative-enzymes.com

Contact us

Tel 1-631-562-8517 1-516-512-3133

Website www. creative-enzymes.com

Address 45-1 Ramsey Road, Shirley, NY 11967, USA

Discover more at creative-enzymes.com

16