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GRAM Negative

Positive

Oxidase

Catalase

Negative (Bacillus)

Positive

MacConkey Negative

Positive

Haemophilus influenzae

Lactose

Factors V & X Negative Sorbitol / HEK (H2S)

Negative Shigella

Bacillus

Coccus

MacConkey

Glucose +

Negative

Positive

Positive Vibrio cholera Pseudomonas aeruginosa TCBS + (yellowcolonies) Dextrose +

Positive Negative Salmonella

Negative

EHEC

Positive All others

Positive

α hemolytic β hemolytic γ hemolytic Optochin

Susceptible

Enterococcus faecalis Salt tolerant Bile Esculin +

Resistant

Streptococcus Streptococcus Bacitracin pneumoniae viridans Maltose

Negative Neisseria gonorrhoeae

Positive Neisseria meningitidis

Susceptible CAMP --

Bacillus

Coccus

β hemolytic

Clusters

Listeria monocytogenes

Coagulase

Motility SIMS +

Resistant CAMP + Susceptible

Streptococcus pyogenes

Streptococcus agalactiae

Coagulase Negative Staphylococcus

Negative

Positive

Novobiacin

β hemolytic

Resistant

Staphylococcus aureus

Staphylococcus saprophyticus

Bacterial flow chart  
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