Page 1

T20

TM

Plasmid DNA/mRNA Transfection


293T: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells per well : 5 x 10

4

pDNA per well : 1 µg

Figure 1: GFP Expression by T20™ reagent in 293T cells after 48 h.


293T: 48 h post transfection of pDNA

669 600 500

T20™ Control

Count

400

GFP Positive:

300

93.02%

200 100 0

1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in 293T cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 93% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

73%

20 0 Control

T20™

Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected 293T cells are 73% viable after 48 h.


9L: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells seeds per well : 5 x 10

4

pDNA per well : 1 µg

Figure 1: GFP Expression by T20™ reagent in 9L cells after 48 h.


9L: 48 h post transfection of pDNA

673 T20™ Control

Count

500

GFP Positive:

400

82.98%

300 200 100 0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in 9L cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 82% of cells fluoresce after 48 h.

100

% Viablility

80 60 40

100%

92%

20 0 Control

T20™

Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected 9L cells are 92% viable after 48 h.


A172: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells per well : 3 x 10

4

pDNA per well : 1 µg

Figure 1: GFP Expression by T20™ reagent in A172 cells after 48 h.


A172: 48 h post transfection of pDNA

948 T20™ Control

800 600

GFP Positive:

Count

74.51%

400 200 0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in A172 cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 75% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

93%

Control

T20

20 0 Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected A172 cells are 93% viable after 48 h.


A375: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells per well : 5 x 10

4

pDNA per well : 1 µg

Figure 1: GFP Expression by T20™ reagent in A375 cells after 48 h.


A375: 48 h post transfection of pDNA 532 500

T20™ Control

400

GFP Positive:

Count

300

72.29%

200 100 0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in A375 cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 72% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

96%

20 0 Control

T20™

Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected A375 cells are 96% viable after 48 h.


A549: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells seeds per well : 3 x 10

4

pDNA per well : 2 µg

Figure 1: GFP Expression by T20™ reagent in A549 cells after 48 h.


A549: 48 h post transfection of pDNA

771 T20™ Control

Count

600

GFP Positive:

400

70.10%

200

0 10

1

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in A549 cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 70% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

92%

20 0 Control

T20™

Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected A549 cells are 92% viable after 48 h.


C2C12: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells seeds per well : 3 x 10

4

pDNA per well : 2 µg

Figure 1: GFP Expression by T20™ reagent in C2C12 cells after 48 h.


C2C12: 48 h post transfection of pDNA

632 T20™ Control

500

Count

400

GFP Positive:

72.70%

300 200 100 0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in C2C12 cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 73% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

81%

Control

T20

20 0 Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected C2C12 cells are 81% viable after 48 h.


C6-S: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells per well : 2 x 10

4

pDNA per well : 2 µg

Figure 1: GFP Expression by T20™ reagent in C6-S cells after 48 h.


C6-S: 48 h post transfection of pDNA

871 T20™ Control 600

GFP Positive:

Count

75.88%

400 200 0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in C6-S cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 76% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

91%

Control

T20

20 0 Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected C6-S cells are 91% viable after 48 h.


CHO-K1: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells seeds per well : 3 x 10

4

pDNA per well : 2 µg

Figure 1: GFP Expression by T20™ reagent in CHO-K1 cells after 48 h.


CHO-K1: 48 h post transfection of pDNA

774

Count

600

T20™ Control GFP Positive:

400

66.15%

200

0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in CHO-K1 cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 66% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

97%

20 0 Control

T20™

Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected CHO-K1 cells are 97% viable after 48 h.


COS-7: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells seeds per well : 5 x 10

4

pDNA per well : 1 µg

Figure 1: GFP Expression by T20™ reagent in COS-7 cells after 48 h.


COS-7: 48 h post transfection of pDNA

719 T20™ Control

600

Count

GFP Positive:

80.47%

400

200

0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in COS-7 cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 80% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

96%

20 0 Control

T20™

Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected COS-7 cells are 96% viable after 48 h.


H4: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells seeds per well : 5 x 10

4

pDNA per well : 1 µg

Figure 1: GFP Expression by T20™ reagent in H4 cells after 48 h.


H4: 48 h post transfection of pDNA

663 T20™ Control

500

Count

400 GFP Positive:

90.83%

300 200 100 0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in H4 cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 90.83% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

92%

Control

T20

20 0 Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected H4 cells are 92% viable after 48 h.


HEK: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells per well : 2 x 10

4

pDNA per well : 2 µg

Figure 1: GFP Expression by T20™ reagent in HEK cells after 48 h.


HEK: 48 h post transfection of pDNA

678 600 T20™ Control

500

Count

400

GFP Positive:

300

93.06%

200 100 0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in HEK cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 93% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

82%

20 0 Control

T20™

Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected HEK cells are 75% viable after 48 h.


Ht1080: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells per well : 5 x 10

4

pDNA per well : 1 µg

Figure 1: GFP Expression by T20™ reagent in Ht1080 cells after 48 h.


Ht1080: 48 h post transfection of pDNA

454 400 T20™ Control

Count

300

GFP Positive:

97%

200 100 0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

7.2

10

Figure 2. Expression of GFP in Ht1080 cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 97% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

75%

Control

T20™

20 0 Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected Ht1080 cells are 75% viable after 48 h.


MCF-7: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells per well : 2 x 10

4

pDNA per well : 2 µg

Figure 1: GFP Expression by T20™ reagent in MCF-7 cells after 48 h.


MCF-7: 48 h post transfection of pDNA

481 400

T20™ Control GFP Positive:

Count

300

79.06%

200 100 0

1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in MCF-7 cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 79% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

95%

20 0 Control

T20™

Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected MCF-7 cells are 95% viable after 48 h.


MDCK: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells seeds per well : 2 x 10

4

pDNA per well : 2 µg

Figure 1: GFP Expression by T20™ reagent in MDCK cells after 48 h.


MDCK: 48 h post transfection of pDNA

556 T20™ Control

Count

400

GFP Positive:

300

68.57%

200 100 0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in MDCK cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 69% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

97%

20 0 Control

T20™

Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected MDCK cells are 97% viable after 48 h.


NIH 3T3: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells seeds per well : 3 x 10

4

pDNA per well : 1 µg

Figure 1: GFP Expression by T20™ reagent in NIH 3T3 cells after 48 h.


NIH 3T3: 48 h post transfection of pDNA

520 T20™ Control

400

GFP Positive:

Count

300

54.98%

200 100 0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in NIH 3T3 cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 55% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

96%

20 0 Control

T2™0™

Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected NIH 3T3 cells are 96% viable after 48 h.


RG2: 24 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells per well : 3 x 10

4

pDNA per well : 1 µg

Figure 1: GFP Expression by T20™ reagent in RG2 cells after 24 h.


RG2: 24 h post transfection of pDNA

901 T20™ Control 600

GFP Positive:

Count

48.63%

400 200 0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in RG2 cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 49% of cells fluoresce after 24 h.

% Viablility

100 80 60 40

100%

90%

Control

T20

20 0 Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected RG2 cells are 90% viable after 24 h.


T98G: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells per well : 6 x 10

4

pDNA per well : 1 µg

Figure 1: GFP Expression by T20™ reagent in T98G cells after 48 h.


T98G: 48 h post transfection of pDNA

642 T20™ Control

500

Count

400 GFP Positive:

300

74.54%

200 100 0 1

10

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in T98G cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 75% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

94%

20 0 Control

T20™

Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected T98G cells are 94% viable after 48 h.


U87: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells per well : 6 x 10

4

pDNA per well : 1 µg

Figure 1: GFP Expression by T20™ reagent in U87 cells after 48 h.


U87: 48 h post transfection of pDNA

722

Count

600 T20™ Control

400

GFP Positive:

93.02%

200

0 10

1

10

2

10

3

4

10 10 GFP-H

5

10

6

10

7.2

Figure 2. Expression of GFP in U87 cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 93% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

97%

20 0 Control

T20™

Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected U87 cells are 97% viable after 48 h.


U118: 48 h post transfection of pDNA Medium per well : 1 ml

Plate type : 12-well Cells per well : 6 x 10

4

pDNA per well : 1 µg

Figure 1: GFP Expression by T20™ reagent in U118 cells after 48 h.


U118: 48 h post transfection of pDNA 780 600

GFP Positive:

Count

74.0%

400 T20™ Control 200

0

1

10

10

2

10

3

10

4

10

5

10

6

10

7.2

GFP-H Figure 2. Expression of GFP in U118 cells quantified by flow cytometry. For cells transfected with GFP expressing plasmid using T20™ reagent, 74% of cells fluoresce after 48 h.

% Viablility

100 80 60 40

100%

97%

20 0 Control

T20™

Figure 3. Cell viability evaluation of control and T20™ reagent by XTT assay.Transfected U118 cells are 97% viable after 48 h.

ALISA Report  
ALISA Report  
Advertisement