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Tissue Culture

About TPP and the color Yellow TPP is internationally recognized by its Swiss quality, the Yellow colored caps and its unequaled performance for tissue culture and laboratory technology. TPP products are widely used in the fields of biotechnology, pharmaceutical, institutes and universities as well as in world wide research organizations. TPP is constantly active in the research and development of new products as well as improvements on design and maintenance of top quality control within the TPP portfolio. These are some of TPP’s goals to ensure that you, the end-user, receives only the best tissue culture plastics for your research, thus significantly promoting consistency and reproducibility in data generation and publication. TPP, the home of tissue culture.

TABLE OF CONTENTS TubeSpin Bioreactors ............................................................................................................. Page 3 Tissue Culture Flasks .............................................................................................................. Page 4 Peel-Off and Re-Closable Tissue Culture Flasks .................................... Page 5 10cm2 “Tiny” Flask ................................................................................................................... Page 6 Tissue Culture Dishes ............................................................................................................. Page 7 Tissue Culture Plates .............................................................................................................. Page 8 Premium 15ml and 50ml Centrifuge Tubes .................................................. Page 9 Serological Pipettes ................................................................................................................... Page 10 Cell Scrapers and Spatula ................................................................................................... Page 11 Rapid Filtermax Bottle Filters ..................................................................................... Page 12 Premium Syringe Filters ...................................................................................................... Page 12 Polar Tubes ......................................................................................................................................... Page 13 PCV Cell Counting Tubes ................................................................................................... Page 13 Tissue Culture Equipment ......................................................................................... Page 14-15

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Midsci

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280 Vance Rd St.Louis, MO 63088

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800-227-9997

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St. Louis: 636-225-9997

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www.midsci.com


TubeSpin Bioreactors

5 cap holes of differing size can be selectively sealed to optimize growth conditions

TPP TubeSpin Bioreactors are ideal for the high throughput screening and optimization of suspension cell system process development. Each bioreactor cap has a built-in 0.22μm hydrophobic membrane to maintain sterility and facilitate gas exchange. Growth conditions can be finely controlled by means of cap holes that can be selectively sealed for optimal gas transfer and media optimization. The simple design contains no internal mixing or stirring mechanism which minimizes cell shearing and foam formation, and agitation is provided by shaking in a CO2 shaking incubator. In this manner, hundreds of cell cultivations can be performed quickly and efficiently in parallel. TubeSpin Bioreactors are also ideally suited for the culture of bacteria, yeast, and other microbiological organisms in suspension. The simple, disposable format offers convenience and time-savings over traditional glass vessels such as test tubes which must be washed and autoclaved before each use. Both aerobic and anaerobic cultures can be grown by selectively covering the holes in the 0.22μm filter cap, offering the flexibility to perform mini plasmid preps, small-scale candidate screens, or other downstream applications. • • • • • •

Maximizes protein yields Optimal gas exchange - cap holes can be selectively sealed 0.22μm hydrophobic cap membrane minimizes evaporation Rapid sampling following centrifugation – entire tube can be sacrificed No foam formation at any speed – no internal stirring mechanism Sterile, certified nuclease, nucleic acid, and pyrogen-free “The TubeSpin Bioreactors are great for our mini bacteria prep. We previously used regular conical tubes, unscrewed and taped the lids to the tube. However in our shaking incubator, the lids would invariably be jarred loose and our cultures would suffer. The TPP bioreactor tubes help us by saving us from taping the lids, and we are confident in the growth of our cells as well.“ – Miroslava Protic, Northwestern University, IL

Order # TP87050 TP87600

Description 50ml TubeSpin Bioreactors 600ml TubeSpin Bioreactors

Qty 180/cs 26/cs

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Tissue Culture

Tissue Culture Flasks

Vented or Filtered Caps

100% access – No “dead” corners Highly visible white graduations and marking area

“TPP flasks are a huge time saver because of their easy access, and you get total cell recovery!“ – Abbigail Montero, Georgetown University, DC

TPP Tissue Culture flasks are designed for superior performance and ease of use, involving the optimization of the culture growth properties, accessibility to the entire growth surface, and convenience for the researcher. • • • • •

• • • •

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No dead corners – 100% cell retrieval with a pipette or cell scraper Absolutely flat growth surface for even, consistent cell growth Highly visible white graduations and marking areas Stable, slip-free stacking of multiple flasks Two cap styles: • Filtered – with a 0.22μm hydrophobic membrane • Vented – audible click and visual indicator prevent over-tightening or under-tightening the flask cap resulting in dead or contaminated cultures Extra-large 300cm2 flask reduces flask usage and saves incubator space Only the bottom surface is opto-mechanically treated to prevent unwanted cell growth up the flask sides Flasks are individually leak tested, and each lot is quality controlled for sterility and cell growth Sterile, certified nuclease, nucleic acid, and pyrogen-free

Order # TP90025 TP90026 TP90075 TP90076 TP90150 TP90151 TP90300 TP90301

Description 25 cm² TC Flask 25 cm² TC Flask 75 cm² TC Flask 75 cm² TC Flask 150 cm² TC Flask 150 cm² TC Flask 300 cm² TC Flask 300 cm² TC Flask

Cap Style Vented Filtered Vented Filtered Vented Filtered Vented Filtered

Qty 360/cs 360/cs 100/cs 100/cs 36/cs 36/cs 18/cs 18/cs

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Peel-Off & Re-Closable Hybrid Tissue Culture Flasks

Re-Closable Lid

The Peel-Off and Re-Closable Hybrid flasks are ideal for transgenic cell construction involving the selection of individual candidate clones post-transfection, or applications such as skin graft cultures encompassing the removal of an entire layer of cells or intact tissue. • Allow complete access to flask chamber for picking clones or scraping, lifting, or treating cells • Quick, easy, and total cell retrieval • Two access options – single use peel-off foil or multi use re-closable lid • Peel-off foil maintains sterility until cells or tissue are harvested • Re-closable lid permits multiple openings and closings under sterile conditions • Flasks have filtered caps with a hydrophobic 0.22μm membrane • 115cm2 flasks have an internal barrier which creates a square (10x11cm) growth area • Sterile, certified nuclease, nucleic acid, and pyrogen-free • Identical footprint as the regular 150cm2 flasks

Peel-Off Foil

“Our lab primarily works with an immortalized neuronal cell line. Although these cells can be sensitive and difficult to propagate, they appear to grow very well on TPP culture dishes. TPP’s flasks with removable lids are very helpful for our research, particularly when we need to harvest cells for RNA isolation.“ – Cheri Goodall, Oregon State University Flasks will not leak in the vertical position with up to 100ml of media

115cm2 Flask internal barrier

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Order # TP90651 TP90652 TP90551 TP90552

Description 115 cm² TC Flask 115 cm² TC Flask 150 cm² TC Flask 150 cm² TC Flask

Cap Style Peel-Off Re-Closable Peel-Off Re-Closable

Qty 18/cs 18/cs 18/cs 18/cs

Sale Price $176.25 $213.03 $176.25 $213.03

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Tissue Culture

10cm2 “Tiny” Flask

Will not fall through a microscope stage like a 35mm dish

Fits standard 50ml tube rotor and can be spun to 1,200 x g

“One of the TPP products we really love is the Tiny 10cm2 Flask. We would always lose cells during transfer and this flask allows us to trypsinize and spin in a single unit and thus avoid transfer and prevent cell loss. This is a great product and we love it.“ – Svetlana, MD Anderson Cancer Center, TX

10cm2 tear-drop growth surface can be exactly worked with a cell scraper

Grow and spin cells in the same vessel!

The innovative design of the 10cm2 Tissue Culture flask incorporates the features of a tissue culture flask and a 50ml conical centrifuge tube into a single multi-functional culture vessel. • • • • • • •

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10cm2 growth area – great for bringing up cells from frozen storage or for culturing primary cell lines Fits standard 50ml tube rotor – can be spun up to 1,200 x g The drop-shaped growth surface can be exactly worked with a cell scraper Flat viewing window with excellent optical clarity for microscopy White graduations and marking area Scan to Learn More 0.22μm hydrophobic filter cap allows optimal gas exchange and prevents evaporation Sterile, certified nuclease, nucleic acid, and pyrogen-free

Order # TP91253

Description 10 cm² TC Flask

Cap Style Filtered

Qty 350/cs

Sale Price $255.26


Tissue Culture Dishes

Stable stacking and impeccable optical clarity

“The majority of work we do is tissue culture. The TPP products make the process so much simpler with minor adjustments that make major differences. We especially love the ridges along the covers of the 10cm2 plates. It makes them easier to grasp and reduces slipping when the plates are stacked. We also really like the yellow strips for labeling the plates. We can see which sample we want without taking them all out of the incubator. We wouldn’t use any other product for our tissue culture.“ – Amy Clem, University of Louisville, KY

Numeric “Clock” Orientation Markings

Air vents in dish base prevent condensation and provide consistent growth conditions even when dishes are stacked

Serrated Grip-Edge

Unique “Grip-Edge” Eliminates Dropped Dishes and Accidental Contamination. Innovative features and outstanding cell growth properties make the TPP Tissue Culture Dish the ideal choice for adherent cell culture in academia and industry. • • • • •

Serrated grip-edge prevents accidental dropping of the dish bottom Double marking areas – yellow on side of top, and frosted on side of bottom Numeric “clock” markings (12, 3, 6, 9) divide dishes into quadrants for quick orientation Ridged dish bottoms provide stable, slip-free stacking of dishes Vent base permits continuous circulation of air between stacked dishes for consistent growth conditions and prevention of condensation • Excellent flatness and optical clarity • Packaged in sterile sleeves • Certified nuclease, nucleic acid, and pyrogen-free Scan to Learn More

Order # TP93040 TP93060 TP93100 TP93150

Description 40 mm TC Dish (9.2 cm² growth surface) 60 mm TC Dish (22.1 cm² growth surface) 100 mm TC Dish (60.1 cm² growth surface) 150 mm TC Dish (147.8 cm² growth surface)

Qty 900/cs 840/cs 240/cs 100/cs

Sale Price $244.01 $240.32 $126.02 $152.09

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Tissue Culture

Tissue Culture Plates

Air vents in plate base prevent condensation and provide consistent air-flow between stacked plates

“We like TPP because the flatter growth surfaces allow us to plate our fibroblasts more evenly and efficiently, giving us a higher yield of Toxoplasma gondii parasites than any other cell culture manufacturer. This also allows for even treatment of our parasites, and a more consistent growth environment. We like the TPP plates for there grip edge, ensuring the lids stay on and prevent contamination. The quality and clarity of TPP plastic is the best in the industry, allowing us to clearly see and evaluate our parasite under magnification.“ – Kyle Boldon, University of Wisconsin-Madison, WI

• • • • • • • • •

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Yellow marking areas

Black alphanumerics on top for quick and easy identification of wells Easy-to-see black alphanumerics Clear alphanumerics between wells on the bottom for easy orientation during microscopy Yellow marking areas on lids and plates make labeling easy and also ensure correct lid orientation Textured easy-grip area ensures a secure grasp and prevents from accidentally lifting off the lid Each plate has a vented base that guarantees secure stacking while allowing air flow to prevent condensation and sticking of stacked plates Plate lids have anti cross-contamination rings that line up with the top of each well 96-well plates have a large 340μl capacity, interleaved 2/5 barcode, and are available with either flat or round well bottoms Sterile, packaged individually or in convenient multipacks Certified DNAse/RNAse, and pyrogen-free

Order # TP92006 TP92012 TP92024 TP92096 TP92097 TP92406 TP92412 TP92424 TP92696 TP92697

Description 6-well TC Plate, flat bottom 12-well TC Plate, flat bottom 24-well TC Plate, flat bottom 96-well TC Plate, flat bottom 96-well TC Plate, round bottom 6-well TC Plate, flat bottom 12-well TC Plate, flat bottom 24-well TC Plate, flat bottom 96-well TC Plate, flat bottom 96-well TC Plate, round bottom

Packaging individual individual individual individual individual 4/pk 4/pk 4/pk 6/pk 6/pk

Qty 126/cs 126/cs 126/cs 162/cs 162/cs 64/cs 64/cs 64/cs 96/cs 96/cs

Sale Price $236.81 $236.81 Scan to Learn More $236.81 $293.18 $293.18 $114.33 $114.33 $114.33 $160.90 $160.90


Premium 15ml and 50ml Centrifuge Tubes

White writing label is permanently affixed and can be labeled with any writing instrument

TPP Premium Conical Centrifuge Tubes are designed for exceptional performance that surpasses ordinary 15ml and 50ml conical tubes.

TP99017 racks are autoclavable, alphanumerically labeled, and are collapsible for storage when not in use

“No more cracking at -80°C or buckling during spins!” • • • • • •

Sterile (gamma irradiated) DNAse/RNAse, pyrogen, and heavy metal-free Outstanding transparency White, flat writing area on top for easy labeling Highly visible white graduations 50ml skirted, self-standing tube available

Material Polypropylene (PP) Tubes Polystyrene (PS) Tubes Polyethylene (PE) Caps

Order # TP91115 TP91015 TP91050 TP91051 TP80617

“TPP 50mL conical tubes tubes are great: not only can they withstand extreme cold (far below -80°C), but they also appear to be the only non-animal derived component tube on the market.“ – Humanzyme, IL

Temperature Range -190°C to 121°C -30°C to 70°C -40°C to 80°C

Description 15 ml Conical Tube (PS) 15 ml Conical Tube (PP) 50 ml Conical Tube (PP) 50 ml Conical Tube (PP), with skirt Rack for Conical Tubes

Max. RCF 9,500 x g 1,700 x g n/a

Qty 800/cs 800/cs 360/cs 320/cs 1

50ml and 15ml tubes have graduations all the way down to 0.5ml and 0.1ml respectively

Sale Scan Price to Learn More $277.09 $218.36 $112.19 $108.95 $7.51

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Tissue Culture

Serological Pipettes

The new TPP 25/40ml and 50/70ml Reservoir Serological Pipettes offer large capacity volumes in a compact, ergonomic design. The reservoirs augment the total volume capacity without adding proportionate length to the pipette.

TPP Serological Pipettes fulfill all the requirements for single-use pipetting and accessing tight flask corners during tissue culture: • • • • • • • •

Shorter, ergonomic design for easier maneuvering Bidirectional graduations with crystal clear plastic transparency Absolutely smooth interiors prevent cell accumulation or clumping Low drip-effect tip design Color coded pipettes (cotton plug) for easy identification Stackable dispenser boxes for convenient storage Sterile and individually packaged in peel-off wrapper Certified nuclease, nucleic acid, and pyrogen-free

“Our lab loves the TPP serological pipets. The shorter length is great for pipeting, especially thick samples, as it allows us to get more of our sample back out. The best thing about them however, is the boxes that they come in. We are always cramped for space and the stackable boxes keep the pipets contained in a small space and allow for easier access to refill our pipet racks.“ – Stacey Loberg, Cincinnati Children’s Hospital, OH

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Order # TP94001 TP94002 TP94005 TP94010 TP94024 TP94525 TP94550

Description 1 ml Serological Pipettes 2 mlSerological Pipettes 5 ml Serological Pipettes 10 ml Serological Pipettes 25 ml Serological Pipettes 25/40 ml Serological Pipettes 50/70 ml Serological Pipettes

Qty 400/cs 300/cs 200/cs 200/cs 100/cs 60/cs 50/cs

Sale Price $51.00 $48.00 $49.00 $49.00 $51.00 $52.00 $52.00

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Cell Scrapers and Spatula

“I would highly recommend MIDSCI products to any lab involved in cell culture and molecular biology, especially the TPP cell scraper as it works like an efficient squigy on the bottom of my culture cell dishes. The handle rotates so that you can reach every part of the surface of the dish.“ – Barbara, Medical College of Wisconsin, WI

Pivoting scraper heads are made from a flexible polyethylene material that conforms to the surface for better scraping.

Raised knobs on handle for a more secure grip

Spatula in TPP Peel-Off Flask

TPP Scrapers and Spatula are designed to gently lift off monolayers and leave ‘no cell behind’. • Scraper heads pivot for easy maneuvering and navigation • Scraper blades are made from a specially soft and flexible polyethylene material - maintains great contact but won’t damage cells • Allow for thorough cell retrieval from vessels of many shapes and sizes • Lower contamination risk - pivoting scraper heads negate the need to lift the scraper to re-position • Small raised knobs on handle for a more secure grip • Sterile, packaged in peel-off paper/plastic wrappers Scan to Learn More

Order # TP99002 TP99003 TP99004 TP99010

Description Scraper (24 cm) Scraper (30 cm) Scraper (38 cm) Spatula (19.5 cm)

Head Width 1.3 cm 2.0 cm 2.5 cm 1.4 cm

Qty 150/cs 150/cs 150/cs 150/cs

Sale Price $224.90 $253.76 $210.92 $198.28

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Tissue Culture

Rapid Filtermax Bottle Filters With TPP Rapid Filtermax Bottle Filters, you are guaranteed fast and efficient filtration with convenient storage! • Large, square 49cm2 PES 0.22μm membrane • Rapid filtration times – 20% faster than similar volume units with circular membranes • PES membrane offers extremely low protein binding and excellent chemical resistance • Square bottles are easy to grasp and store more efficiently with less wasted space than circular bottles • Sturdy, square bottle footprint is difficult to tip over during filtering • Screw-cap and vacuum outlet included in each sterile package • 45mm neck thread • Sterile, certified nuclease, nucleic acid, and pyrogen-free

Order # TP99150 TP99250 TP99500 TP99505 TP99950 TP99955

Description 150ml Rapid-Filtermax Bottle Filter 250ml Rapid-Filtermax Bottle Filter 500ml Rapid-Filtermax Bottle Filter 500ml Rapid-Filtermax (bottle-top filter) 1000ml Rapid-Filtermax Bottle Filter 1000ml Rapid-Filtermax (bottle-top filter)

Scan to Learn More

Qty Sale Price 12/cs $88.42 12/cs $99.47 12/cs $128.58 24/cs $193.82 6/cs $116.06 12/cs $192.63

Premium Syringe Filters • • • • • • •

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29mm diameter filters designed to filter aqueous solutions 3 to 5 times faster than other syringe filters Sterile (gamma irradiated) and individually blister packed 0.22-0.45μm PES membrane guarantees extremely low protein binding Standard luer-lock connection Certified DNAse, RNAse, and pyrogen-free Filter rests perfectly on top of a 50ml conical centrifuge tube

Order # TP99722 TP99745

Description 0.22 µm Syringe Filters 0.45 µm Syringe Filters

Qty 40/cs 40/cs

Sale Price $90.00 $90.00

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Polar Tubes TPP Polar Tubes are practical and robust, even under the lowest temperatures. • • • • • • • • •

Externally threaded cryogenic storage vials Temp range: -180˚C to 121˚C Absolute gas-tightness guaranteed without the use of a silicone gasket No more “gasket popping” by over-tightening reduces contamination risk Star-shaped bottom for self-standing and single-handed operation Color-coded cap inserts available Graduated with large writing surface Recommended for vapor-phase LN2 storage only DNAse/RNAse, pyrogen-free Scan to Learn More

Order # TP89012 TP89020 TP99020

Description 1.2 ml Polar Tubes 2.0 ml Polar Tubes Cap inserts for Polar Tubes; 6 assorted colors

Qty 400/cs 400/cs 600/cs

Sale Price $113.69 $113.69 $33.11

PCV Cell Counting Tubes The PCV Easy Read Cell Counting System is designed for accurate cell count measurements with a quick 1 minute spin of cell suspension in the PCV Cell Counting Tube. The resulting cell pellet volume read with the Easy Read measuring device and pre-determined conversion factors will be utilized to calculate cell number, protein production, or metabolic activity. • • • •

No dilutions to obtain optimal counting density No tedious manual hemocytometer counting Cellular aggregates or non-viable cells do not affect counting accuracy Multiple samples can be counted at the same time

Order # TP87005 TP87007 TP87010

Description PCV Cell Counting Tubes (with capillary graduations only) PCV Cell Counting Tubes (no graduations) Easy Read Measuring Device

Scan to Learn More

Qty 50/pk 50/pk 1

Sale Price $98.69 $61.72 $399.68

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Tissue Culture

Shel Lab CO2 WaterJacketed Incubators

• Patented copper-housed HEPA filter for superior contamination prevention • Microprocessor temperature control • Infrared (IR) CO2 sensors • Built-in CO2 tank switcher • Seamless stainless steel interior for easy cleaning • Made in USA • 5-year warranty (7-years on IR sensor)

ArcticTemp™ -85°C Ultra-Low Freezers

• • • •

Upright or chest models Digital temperature control Temperature range: -40°C to -85°C CFC & HCFC-free refrigerant with energy saving compressor • Battery operated alarm system with audio and visual alarms • Automatic timer system will restart the unit in case of power failure • 1-year warranty

• • • •

Easy-to-Clean stainless steel tank Air jacket heating eliminates hot spots Available in four sizes (2, 6, 14, and 20 L) Recessed heating element prevents burnout • Pocket handles for easy lifting • Made in USA • 1-year warranty

Lab Armor® Beads for Water Bath

• Not prone to microbial contamination like water • Bottles or tubes will not tip over and float • No more emptying, cleaning, or refilling the water bath • Eliminates water evaporation and burnout worries • More energy efficient than water • Better temperature flexibility - can go hotter than boiling • Made in USA

Taylor-Wharton Cryogenic Dewars

Drummond Portable Pipet-Aid® XL

• • • •

• Ergonomic, innovative design • Three speed settings for precise control • Longer handle lowers your arm position to reduce head, neck, and arm strain • New quiet pump increases efficiency and reduces noise • Can be charged while in use to eliminate downtime • Supplied complete with charger, 4 extra filters, and wall bracket • Made in USA • 1-year warranty

LN2 storage of cell stocks Storage capacities from 150-80,000 vials Rugged construction Superior vacuum performance and insulation for maximum holding times • Optional low-level alarms available

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Shel Lab Digital Water Baths


Infinity Rocker

Digitally controlled speed, up to 80 cycles/min Available in multiple sizes and with 3 and 4 platforms Adjustable tilt from 1 to 18 degrees Designed to last, rugged construction Made in USA 4 year warranty

• Includes 4 AlphαPettes: 10µl, 20µl, 200µl, 1000µl • 3 Racks of Avant tips: 10µl, 200µl, 1250µl • 6-Place carousel pipettor stand and Backpack

Hermle Z400 Ambient and Z400K Refrigerated Centrifuges

Orbi-Shaker CO2 Resistant Shaker w/ Remote Control

• Multiple swing-out and fixed angle rotor options • Max RCF: 17,310 x g • G-force conversion software, rpm or RCF display • Maintenance-free induction drives provide quiet operation • Imbalance sensor and rotor recognition • Timed, momentary, or continuous operation • 2-year warranty

• Designed for use in CO2 incubators • Remote controllable; adjust settings without opening incubator door • Change flask clamps almost instantly with MAGic Clamp magnetic platforms • 2-year warranty

Drummond Pipet-Aid XP2

AlphαPette Pipettors Starter Kit

NEW!

• NEW Ergonomic Design; the most comfortable pipettor you ever laid a hand on • Power Source for uninterrupted extended operation; can be charged while in use • Ultra quiet precision pump; great control for aspiration or dispensing • Made in USA • 1-year warranty

Vanguard Inverted Microscopes

• Vivid optics for advanced tissue culture work • Plan achromatic, infinity corrected optics • 4X, 10X, 20X, and 40X objectives • Ergonomically positioned focusing controls • Brightfield and phase-contrast models • 5-year warranty

Available at www.midsci.com or by calling 800-227-9997

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NEW!

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“The TPP PCV tubes are a great convenience in our labs, easy to use and efficient. These tubes offer a tremendous speed advantage over classical hemocytometer counting. You can process as many tubes as your centrifuge’s router has spots for them in minutes. Count even few flasks worth of cells and your time savings is near 10x. The PCV tube is an ingenious device that allows users to quantitate cells/volume based on a measurement of the packed cell volume and comparing that against a standard – this process is a great addition to any lab that has a need to count cells.“ – Chuck Fontaine, Diagnostic Hybrids, A Quidel Company

PRSRT STD BULK U.S. POSTAGE PAID ST. LOUIS, MO PERMIT NO. 652

280 Vance Road • St. Louis, MO 63088

custserv@midsci.com • www.midsci.com 800-227-9997 • 636-225-9997 • Fax: 636-225-9998

Lab Armor Beads

ELIMINATE WATER… ELIMINATE CONTAMINATION! • Noneedformicrobicidesorotherharmfulchemicals •Savestime–eliminatesemptying,cleaning,andrefilling waterbaths •Eliminatesbathburnoutrisk •Sampleswon’tfloatortipover •Energyefficient–requirelessenergytomaintain temperaturethanwater •Forhotorcoldincubations

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Midsci TPP 2011 Catalog