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Infrastructure and equipment of the laboratory for handling cell cultures. Obtaining and production of primary cultures from any organ of human or animal species. Maintenance of mammalian cell lines (VERO, Hep2, McCoy, BHK21, RFT18, CACO2MDBK, MDCK, etc.) Maintenance of invertebrate cell lines. Karyotypic characterization of cells in culture (modal class). Evaluation and Control of contamination in culture rooms and cells ( viruses, Mycoplasma sp., Fungi). In Vitro cell transformation. Freezing and thawing of cell lines (Cell line bank). Maintaining of Myelomas and Hybridomas cell lines for (monoclonal Abs bank). Establishment and characterization of new cell culture lines. Multiparameter studies, viability and apoptosis by flow cytometry. Production to median and large-scale (Rolex, fermenters ). Study of cell ultrastructures by transmission electron microscopy.

www.aleidavillaespinosa.com

www.aleidavillaespinosa.com


Method for scrubbing of tissue culture glassware using trisodium phosphate (1980) BB82. New line cell culture for virus studies (1982) Obtaining primary cell cultures from cells and recovered media (1983) Obtaining and characterization of cell culture line PILI001, persistently infected with Lawsonia intracellularis. New biological system for the production of vaccines. (2001) COE (Lamb umbilical cord cells). New cell Line continues for studies of Protozoa (2009) MONO. Bovine Mononuclear diploid cell line, obtain by clonal cell expansion by Theileria annulata. (2010).

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Isolation and identification of Astrovirus

Isolation and identification of Feline Calicivirus

Isolation and identification of Rotavirus type A

Isolation and identification of Feline Parvovirus (FPV)

Isolation and identification of Pestivirus (BVDV, BDV, CSFV)

Isolation and identification of Canine Parvovirus (CPV)

Isolation and identification of BHV type I

Isolation and identification of Canine Coronavirus (CCoV)

Isolation and identification of BHV type II

Isolation and identification of Feline Coronavirus (FCoV)

Isolation and identification of Bovine Leukemia virus ( BLV) Isolation and identification of Equine Anemia virus (AIEV) Isolation and identification of Smallemberg virus (VSB) Isolation and identification of PRRSV Isolation and identification of BRSV

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Isolation and identification of Canine Distemper (CDV) Isolation and identification of Caprine Herpesvirus (CpHV-1) Isolation and identification of Para Influenza III (PI 3) Isolation and identification of Porcine parvovirus (PPV) Isolation and identification of Porcine Circovirus (PCV-2)


Isolation and identification of Aujeszky disease (PHV-I) in cell culture and laboratory animals

Isolation and identification of Encefalomiocarditis virus (EMCV) in cell culture and laboratory animals Isolation and identification of AvĂ­an Infectious Bronchitis (AIBV) in cell culture and chicken embryos Isolation and identification of Newcastle disease virus (NDV) in cell culture and chicken embryos Isolation and identification of Coxiella burnetii in cell culture and chicken embryos Isolation and identification of Chlamydias and Chlamydophilas in cell culture and chicken embryos

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Isolation and identification of Theileria annulata from clinical samples. Culturing and identification of Cryptosporidium sp. from clinical samples. Isolation and identification of Encephalitozoon cuniculi from clinical samples. Isolation and identification of Babesia sp (B. bovis and B. bigemina) from clinical samples. Isolation and identification of Anaplasma marginale from clinical samples. Isolation and identification of Toxoplasma gondii. from clinical samples .cell culture and mouse. Isolation and identification of Besnoitia besnoiti from clinical samples. Isolation and identification of olation and identification of Eimeria sp. from clinical samples.

Isolation and identification of Trypanosome sp. from clinical samples Maintenance and passage of Leishmania infantun strain in cell culture.

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Production of medium-scale of infected cell cultures for the production of viral vaccines Recombinant antigens FOR PRODUCTION OF VACCINES Design and development of a vaccine against Coxiella burnetii in VERO cell line Design and development of a vaccine against Chlamydia sp. in Mc Coy cell line Design and development of a vaccine against Bovine Tristeza from cell cultures infected ticks Development of Cryptosporidium sp vaccines in COE cell line Development of Encephalitozoon cuniculi vaccines in COE cell line

Development of Besnoitia besnoiti vaccines in COE cell line Development of Toxoplasma gondii vaccines in COE cell line Development of Babesia sp vaccine in primary culture from peripheral blood of infected cattle Development of Anaplasma marginale in primary culture from peripheral blood of infected cattle

Development of primary cultures of infected lymphocytes Theileria annulata for obtaining vaccines

Production of medium-scale of Brachyspira sp. autovaccines Commissioning and production of bacterin for pathogenic Leptospira. www.aleidavillaespinosa.com


Experiencia en biologia celular y microbiologia