Gel permeation chromatography (GPC) or size exclusion chromatography is a kind of liquid chromatography technique that separates analytes based on their size. This chromatography technique employs a stagnant liquid as the stationary phase that is present in the pores of beads and a flowing liquid as the mobile phase. Historically, the porous medium used in GPC was composed of a gel. Hence, the term gel permeation chromatography (GPC). Since, GPC is convenient, reproducible, simple, rapid, and reliable, it is expected to be generally applicable in various biochemical as well as clinical research. Currently, the market for gel permeation chromatography is expected to grow significantly due to the exponentially growing biotechnology and pharmaceutical industry along with increasing application of chromatographic technologies in various sectors of the healthcare industry.