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Skin Remodeling Demonstration in Mice: Hyaluronic Acid Introduction using the AirgentTM Professor Yasuo Sakai , Associate professor , Plastic surgeon


Abstract Background: The Airgent™ is a novel skin-remodeling platform designed to introduce and laterally disperse a therapeutic substance that initiates a wound healing process in the dermis and mechanically augments collagen regeneration Objective: To determine the efficacy of the AirgentTM procedure as a long-term skin remodeling solution


Methods • • •

48 mice (7-9 weeks old; 25-35g weight) 2 cm incision on the back Biopsies, evaluation of collagen types I and III generation


Immediately Following a Single Shot Ă—4

Ă—4

Control

Treated Site


Changes Observed in Tx Area (1 & 2 weeks) 1 week following tx ×10

2 weeks following tx ×10


Changes Observed in Tx Area (4 weeks) ×4

×10

Control

Treated Site


Changes Observed in Tx Area (8 weeks) ×4

×10

Control

Treated Site


Dermis Thickness (weekly analysis)

Cont(N=2)

Control

Treated Site

281.3

468.8 **

1W (N=10) 340.2 ±25.4

445.1 ±64.7 **

2W (N=12) 361.9 ±40.0

442.0 ±104.6 **

4W (N=12) 366.5 ±51.6

524.0 ±89.2 **

8W (N=12) 474.0 ±51.4

603.4 ±98.9 ** p<0.01


Sirius Red Staining (2 weeks) Collagen I

Collagen III


Sirius Red Staining (4 weeks) Collagen I

Collagen III


Summary • HA Solution exhibits uniform spread and coverage

of the treated site • Dermal thickening is significantly demonstrated • An increase in Collagen type III was evident 2 weeks following treatment • An increase in Collagen type I was evident 4 weeks following treatment • Collagen regeneration was evident for at least 8 weeks following treatment


Mice Study - Airgent Jan2009