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Cell Culturing - Cell Culture Flask _____________________________________________________________________________________ By Stephen - Cell line - Specific cells that can grow indefinitely given the appropriate medium and conditions. The significance in cell culturing that a cell line may have has to do with the purity and sterility of the original cells. If the cell line is not pure and has been exposed to other cells the experiment will be skewed. If an experiment is requiring a specific cell type the cell line is most important to remain the same and in its sterile form. If the cell line has been exposed to other cells it may mutate into another form and the experiment is no longer prevalent. What Is Cell Culture Flask Subculture - Splitting or passage, the transfer of cells from one culture vessel to another. Usually this involves a proliferating cell culture that has reached confluence. It is important if establishing a tissue culture and it helps to keep the cells healthy and actively growing. It is also important for use of cells in future experiments.Confluent - The state in which cells have grown to maximum capacity within a certain amount of space. At this point surface to surface contact with other cells causes them to inhibit growth. Subculturing is important at this juncture to ensure that the individual cells are receiving the proper media innoculant. Confluency lets the scientist know when it is time to subculture because the cells have proliferated enough to become a detriment to one another.

Trypsinization - The enzymatic dissociation of cells from their culture vessels during subculture. In order to remove the cells from the walls of the container or slide, trypsin must be used. It can be supplemented with enzymes, EDTA, or chelating agents. The ideal situation is to dissociate and separate cells in the shortest amount of time with the least harmful method possible.

Hemacytometer - A glass slide with a tiny grid etched onto the surface; for use in counting cells. When counting cells in an experiment when studying cell death for instance, it is important to get a quantitative amount of cells that survive. A hemocyto- meter helps evaluate the quantitative aspects of an experimentWhat is cell culturing? The process of removing cells from a living organism and growing them in controlled conditions (a culture). It is the method used to maintain cell lines.

What is the scientific benefit of growing cells in a culture? It is beneficial in culturing highly specialized cells under strictly defined conditions. It allows quick access to growing cells to observe morphological changes. A greater understanding of the workings of the cell can be accomplished using this method without harm to a living being. Research on cells has the potential to lead to new treatments for a range of serious human ailments, including diabetes, spinal cord injury and burns, cancer, etc. Describe the aseptic technique used in cell culturing. Aseptic technique is the removal of the possibility of microbial infection. This technique is done in a sterile hood that provides a microbe-free workspace. The hood has a laminar flow of well-filtered air that prevents the influx of microorganisms into the work space. One must always use gloves and change them frequently. Wipe hood and all surfaces with 70% ethanol. Make sure the work place is cleared of clutter under the hood. The work area should be far from drafts and traffic flow.

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Cell culture flask