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The following data is the detail translation from video taken in Dr.沈湯龍’s class for key points of 2nd molecular biology.

It is strongly suggested taken with textbook – Gene x


Ch-19

RNA-polymerase

Page

contents be aware of

??

transcription bubble

p.510

polymerase subunits 組合

p.511

σ factor 功能 (不是 ơρ 歐)

p.512

polymerase 是用 direct displacement 而非 sliding 移動

p.518

σ factor 的結構和其 domain 的功能

p.519

σ factor 的調控機制 (N-Terminals blocks….)

p.521&522

一些基礎分生技術 (e.g footprinting?? Modification mapping binding site in two ways?)

p.524

RNA polymerase 結構 (DNA 進來碰到結構順序: bridge-wall-rudder , what r they??)

p.526

what should RNA terminator look like?

p.527&528

what should rho protein look like and how it acts ??

p529

what is “antitermination complex” ?


Ch-26 The Operon Page

contents be aware of

p.738

basic concept of regulation

p.739

fig26-4 is important (draw)

p.740

fig26-5 is important (draw),which is about the full structure of lac operon (include the protein it codes?)

p.743

section 26-4, the allosteric control of repressor protein

p.744~745

genetic approach to study lac operon, remember the mutants!!

p.750

about 3 operator (o1,o2,o3) it binds and the repression efficiency縲[echanic to regulate

p.751

three important factors for repressor efficiency 1.genome size, 2.repressor number, 3.affinity (by inducers) How to make the calculation ??

p.753

induced or repressed ? where should u put the CAT (CRP) into the lac operon ??

p.757~759

how E.coli control trp operon (draw) what is leader peptide sequence and how it works

p.760

how is translation by proteins ? what is autoregulation??


Ch-27 Phage strategies Page

contents be aware of

p.772

initial control (change σ factor?) and

termination control

(anti-terminator protein stop transcription termination) p.776~778

section 27-7

lytic cycle

section 27-8

lysogenic cycle

p779

section 27-9

what is immunity region??

p.781

competetion between “cro” and “cI”

P.782

Binding affinity of o1,o2,03.

p.784

competition in lytic cycle and lysogenetic cycle

p.785~787

section 27-15 CII, CIII –establishment of lysogeny CI

-maintenance

Section 27-16 What is CII function ?? (Promoter RE is poor fit (atypical -10 &-35 sequences) -require CII (positive regulatr) to help RNA pol to bind.) p.789

cro repressor for lytic cycle (fig 27.34 (lytic) and cf fig 27.33( lysogenetic ) important , fig 27.35(sum up))


Ch-30 RNA Regulators Page

contents be aware of

p.863

what is ribozyme? What is aptamer ?

p.867-868

noncoding RNA , and make example from bacteria Srna (oxyS RNA)

P.870-871

fig 30.9 and 30.10 is important How miRNA, siRNA, piRNA be synthized and where are they from, what is the enzyme and protein in the process??

P.873

translation repression concepts

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It is strongly suggested taken with textbook – Gene x The following data is the detail translation from video taken in Dr.沈湯龍’s class for ke...

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