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Florida Department of Law Enforcement STR SUMMARY SHEET

(Ft,LE

Case Number:

20120406772

S amp l e ID

3

swab casings

Description Run

0851179 021511

.

.

31-Jan-13 nsd nsd

075820

nsd

0351358

nsd

C5F1PO

nsd

TH01

nsd

0165539

nsd

0135317 0251338

nsd nsd

0195433

nsd

TPOX

nsd

vWA

nsd

018551

nsd

055818

nsd

AMEL FGA

Date:

nsd nsd

Sample ID Run

0851179 021511

075820

C5F1PO

0351358 TH01

0135317

0165539 0251338

0195433 vWA

TPOX

018551 AMEL

055818 FGA

Approved 01 May 2012

02/11/13

-:-··

Initials:

FDLE#:

D2te:J

I

Date:

--

spot I tube I

overiayf

a-napt

�-

\�

FDLE Case #:

�o \d-. oL-io l_p T--:=t �

Area Cleaned

P3n I HEMATRACE

sf

Po

Oitho-di'l.: 1 .

.,

I

I$$Uet! 'from 8WN10 March 25, 20i0

Date:/

Fcr.P30:

Ex!;. Da:e: .

I

.

.

PHADEBAS.

1:50j

K! Lot[

Ser.le!i

:

.

O.SM ..

... . .. · -·

. .. . -

Florida Department of Law Enforcement Biology Section

FD ·.

Date:

.

C;i

SAMPLE PREPARATION WORKSHEET DNA IQ™ LYSIS BUFFER METHOD- NON-DIFFERENTIAL

...

.

/

Area:

Analyst:

/

K

Cleaned:

-Vol. Case # I Comments

Sample I D I Description

lsw20120406772_Ex3

lsw20120406772_RBQ

1t

(IJL)

Sample I D I Description

Case # I Comments

-Vol. (�L)

lsw20120406045 Ex1 lsw20120406045 RBQ

lsw20120406801_Ex1

lsw20110404779_Ex9.1

lsw20120406801_Ex2

lsw20110404779_Ex9.2

lsw20120406801_Ex5

lsw20110404779_RBQ

J.

lsw20120406801_Ex6 lsw20120406801_Ex7

?.

lsw20120406801_Ex8

-- :·-1 ; '

lsw20120406801_RBQ

Initials 1

Place the sample cutting into a sterile extraction tube.

2

3 4a

. ..;;) Add 1001JL DNA IQ™ Lysis Buffer with DIT . to the sample tube. "7) Vortex and b �iefly centrifuge to force the cutting into the buffer.

I

/

4b

� 6

Temperature: Start Time:

Incubate in a dry heat block at 95°C for approximately 30 minutes.

Stop Time: Temperature:

General

..

----;. 0

'10

Start Time:

Incubate in a dry heat block at 70°C for approximately 30 minutes to overnight.

Stop Time:

.:.:-s

, ·:-1

:

r

.

Allow the sample to reach room temperature and briefly centrifuge to remove any condensation from the cap.

.;!f\

2

Optional: Transfer the substrate into a spin basket. Centrifuge at maximum speed for

Remove the basket from the extract tube and retain the substrate.

Date:

I

Analyst:

Microcon Lot:

I

Area:

·,�0

\ � G\l

Cleaned:

DNA IQ™ Lysis Buffer with OTT Lot:

Comments:

Note: Samples can be left in the buffer for a maximum of 48 hours prior to the start of automated extraction by the BIOMEK® 3000.

-

·;

IQ™ Lysis Buffer with OTT Lot:

FTA Cards

Optional: DNA Concentration I Wash

B

DNA

Pipette a maximum of 100�L of the sample lysate into the

. .

.

appropriate wells of a 96 deep-well plate.

BIO PreProcNonDiff2col (14)1

Time I Date Prepared: Time I Date Loaded�

I

I

:

Issued from BEW1 o March 25, 201 o

(-

----

·-

-- -- ---·-- --

CFDLE

--

--

-

-. --

---- -

-

Florida Department of Law Enforcement Biology Section

Biomek® 3000 Extraction Set

Description

Sample ID

-

Case #I Comments

Sample

KIQ

Date:

B3K Plate Name:

ID

Description

30-Jan-13

Case # I Comments

KIQ

1 lsw:20120406772 Ex3

Q

41 edmh 20120407111 3

Q

2 lsw20120406772_RBQ

Q

42 edmh_20120407111 4

Q

3 lsw20120406801_Ex1

Q

43 edmh_20120407111 RBQ

Q

4 lsw20120406801_Ex2

Q

44 edmh_20120407113_1

Q

5 lsw20120406801_Ex5

Q

45 edmh_20120407113_2

Q

6 �w20120406801 Ex6

Q

46 edmh_20120407113_RBQ

Q

7 lsw20120406801_Ex7

Q

47 edmh_20120402713_4

Q

8 lsw20120406801 Ex8

Q

48 edmh_20120402713_RBQ2

Q

9 lsw20120406801 RBQ

Q

49 edmh_20130400029 1

Q

1 0 lsw20120406045 Ex1

Q

5 0 edmh_20130400029_2

Q

1 1 lsw20120406045 RBQ

Q

5 1 edmh 20130400029 3

Q

1 2 lsw20110404779_Ex9.1

Q

5 2 edmh_20130400029_4

Q

1 3 lsw20110404779_Ex9.2

Q

5 3 edmh_20130400029 RBQ

Q

1 4 lsw20110404779 RBQ

Q

54 edmh_20120407111 5 FTA

K

1 5 lsw20090401384_Ex2.3

K

55 edmh_20120407111_RBQ_FTA

K

K

5 6 edmh_20120407111 6

K

K

57 edmh_20120407111_RBK

K

K

5 8 edmh_20120407113_3

K

1 9 lsw20120406801_Ex3

K

5 9 edmh_20120407113_4

K

2 0 lsw20120406801_RBK-FTA

60 edmh_20120407113_5

2 1 lsw20120406801 Ex4

K K

61 edmh 20120407113 RBK

22 lsw20120406801_RBK

K

23. ·lsw20120406045 Ex2

K

63 lsw20130400493 RBQ

24. . lsw20120406045 RBK 25 ·I�W20120406771_Ex1.1

K

64 empty1

K

65 jtm20120404427 2 mn2

plexor only

Q

26 lsw20120406771_RBK

K

66 jtm20120404427_RBQ mn2

plexor only

Q

1 6 lsw20090401384_RBK-3 1 7 lsw20120406645_Ex6

1 8 lsw20120406645_RBK

K

67

28 lsw20120404249_RBK

K

68

29 lsw20120406578_Ex1

K

69

30 lsw20120406578_RBK

K

70

31 lsw20120406998 Ex1

K

71

32 lsw20120406998 RBK

K

72

33 lsw20120407000_Ex1

K

73

34 lsw20120407000 RBK

K

74

Q

76

Q Q

75

36 lsw20120406771_Ex1.2epi 37 lsw20120406771_RBQsp

Q

77

3,8 lsw20120406771_RBQepi

Q

78

·.

39 edmh_20120407111_1

Q

79

40 edmh_20120407111_2

Q

80

/\II II Dill./ n-:rto

K

62 lsw20130400493 Ex2

27 lsw20120404249_Ex5

35 lsw20120406771_Ex1.2sp

K

nrinin�k �rR m�int::�inP.d in the laboratory

p

CFDLE

Florida Department of Law Enforcement Biology Section

™ DNA IQ Lot#:

BIOMEK® 3000 Sample Run Worksheet for DNA IQ

End Column:

and Plexor HY ®

30-Jan-13

DNA IQ Date Run:

0000036314

1X Wash Buffer lot#:

Sample Plate was centrifuged prior to removal of sealing film

Time Run:

&

orientation on deck verified by:

AM

rf

Operator- DNA IQ:

B-158

1

rf rf

7500A

7500 Instrument#: 10

60

B-158

Operator- Quant:

20300

B3K Robot#:

23 Oct 2 0 15

Exp Date: Elution Vol:

Lysis Buffer w/ OTT lot#:

AR30Jan13

B3K Plate Name:

8

11

12

A

B

c

D

E

F

O«J4779_

G

H

RBQ

Ex6

Ex7 H1

Ex2.3 H2

ExB

edmh 201JJ4071 RBK-3

11_6

1.5ml Rack

1

Comments:

A9

Plexor

&

®

1.5ml Rack 4

1.5ml Rack 3

89-plexor only, to be added by operator

HY Lot#:

TE

1 . 5ml Rack 2

empty1

-4

Lot#:

0000042914 8-51 66

Exp Date:

10/22/2013

DNA Standard Lot#:

0000042914

Total Extracted Samples:

1.5ml Tubes for Transfer Method Verified by:

rf

1-

631

CFDLE Autosomal (H)

Florida Department of Law Enforcement

Biomek®

Plexor® HY Results

H- (20 to 21 )

H- (28 to 29}

Exp. Date:

yes

Y- (21 to 22}

Cr value Std. E & E1

yes yes

I D+ Kit Lot #:

yes

Crvalue Std. B & 81 (1 0ng)

yes

Thermal Cycler:

yes

9700 #:

OveraiiiPC Cr Grouped

TmValue

H- (79-81°C)

::.yes *lPG out of range:

TE""' Batch #:

- -

Quant Pit Name:

PHY AR30Jan 13 7500A RF

Quant evaluator:

E DMH Std G 11 omitted

. . . . .. . . .

:

_ __,

.

.

.

:.·

..

..

.

.

deleted black flags

.

Sample Name/ Sample ID

H Quantity (ng/J!l)

Y Quantity (ng/�1)

[Auto]/M

#of

A1

lsw20120406772 Ex3

0.0000

0.0000

0.00

81

lsw20120406772 RBQ

0.0006

0.0000

0.00

2

C1

lsw20120406801 Ex1

1.7800

1.0500

1.70

3

01

lsw20120406801 Ex2

0.4000

0.0390

10.30

4

E1

lsw20120406801 ExS

0.0053

0.0007

7.50

5

0.0170

0.0011

15.40

6

0.0055

0.0004

13.80

7

0.0259

0.0006

41.90

8

0.0000

0.0000

0.00

9

0.0482

2.00

10

F1

lsw20120406801 Ex6

G1

lsw20120406801 Ex?

H1

lsw20120406801 Ex8 lsw20120406801 RBQ

0.0966

A2

.

-

.

....�Rurce Well

. �

A5

Plexor® HY Results

..

82

lsw20120406045 Ex1

C2

lsw20120406045 RBQ

D2

lsw20110404779 Ex9.1

E2 F2 G2

lsw20090401384 Ex2.3

H2

lsw20090401384 RBK-3

. .. ,..

A3

lsw20120406645 Ex6

B3

lsw20120406645 RBK

0.0000

0.0000

0.00

18

.

C3

lsw20120406801 Ex3

2.0400

1.1800

1.70

19

0.0000

0.0000

0.00

20

4.1200

0.0000

0.00

21 22

03 .' . E3

. .'!·� · :

···

.

. -�

-

B-51

Number of Samples to be Amplified:

02,

..

3

N/A

��- Melt (Tml out of N/A . . range: ..

• .·..

1209050 04 Oct 2013

1-15 13

-(79 81°C)

,. -v·- ·

Comments:

AR30Ja n13

Calibration date:

Y- (81-B3°C)

IPC Melt CurveTM

lsw edmh

yes

(0.08ng)

Y- (29 fo 30)

0.0039

0.0033

1.20

11

25.3000

22.9000

1.10

12

lsw20110404779 Ex9.2

0.1250

0.0478

2.60

13

lsw20110404779 RBQ

0.0000

0.0000

0.00

14

9.9900

4.5200

2.20

15

0.0000

0.0004

0.00

lsw20120406801 RBK-FTA lsw20120406801 Ex4

4.8000

2.3300

2.10

16 17

F3

lsw20120406801 RBK

0.0000

0.0000

0.00

G3

lsw20120406045 Ex2

8.8200

6.6200

1.30

23

H3

lsw20120406045 RBK

0.0000

0.0000

0.00

24

A4

lsw20120406771 Ex1.1

13.4000

0.0006

23848.90

25

--·-

30-Jan-13

yes

IPC Verified

yes yes

f.lrr-

yes

R2

yes

83K Plate Name:

rf

-

yes

(H -23-25) Y-lntercept (Y-24-26)

yes

3000 Amplification Worksheet

---- -

Male (Y)

Slope H- {-3 . 3 to -4.0) Y-·(-3.0 to �3.6)

yes yes

j

Biology Section

Operator: Analyst(s): Date Run:

65

· ----

--·-

---·---

Plexor® HY Results

.

. ,

-

·

· ·

.

.

.

.

.

.. .. .

27

C4 04

lsw20120404249 RBK

04

lsw20120404249 RBK

0.0000

0.00

28

E4

0.0000

0.0000

lsw20120406578 Ex1

7.5000

.

-- ·- · ·

.

.

·- ·. -. .. ..

..

.

.

.

.

.

5.3300

lsw2012040657B Ex1

7.5000

4.6400

1.60

29

F4

0.0000

0.0000

0.00

30

lsw20120406578 RBK

lsw20120406578 RBK

G4

lsw20120406998 Ex1

G4

lsw20120406998 Ex1

10.1000

0.0000

0.00

31

H4

lsw20120406998 RBK

lsw20120406998 RBK

0.0000

0.0000

0.00

32

0.0000

H4

85

lsw20120407000 RBK

A5

lsw20120407000 Ex1

19.5000

0.0000

0.00

33

0.0000

C5

6.0300

lsw20120407000 RBK

0.0000

0.0000

0.00

34

lsw20120406771

B5

05

lsw20120406771

12.4000

0.0000

10.1000

6.0300

6.0900

1.00

35

E5

lsw20120406771

12.4000

0.1040

119.90

35

F5

0.0000

lsw20120406771

0.0000

E5

lsw20120406771

0.0000

0.0000

0.00

37

G5

0.0000

0.0000

0.00

H5

edmh 20120407111 1

lsw20120406771

38

0.0385

F5

0.0660

G5

edmh 20120407111 1

0.0385

0.0051

7.50

39

edmh 20120407111 2

A6

0.0108

edmh 20120407111 2

0.0660

0.0115

5.70

40

edmh 20120407111 3

H5

B6

0.0846

A6

edmh 20120407111 3

0.0108

0.0055

2.00

edmh 20120407111 4

41

C6

edmh 20120407111 RBQ

0.0000

B6

edmh 20120407111 4

0.0846

0.0502

1.70

42

06

edmh 20120407113 1

0.0135

C6

edmh 20120407111 RBQ

0.0000

0.0000

0.00

43

E6

edmh 20120407113 2

0.0043

06

edmh 20120407113 1

0.0135

0.0003

48.10

44

F6

edmh 20120407113 RBQ

0.0000

E6

edmh 20120407113 2

0.0043

0.0005

8.50

45

G6

edmh 20120402713 4

0.3300

F6

edmh 20120407113 RBQ'

0.0000

0.0000

0.00

46

H6

edmh 20120402713 4

0.3300

0.2610

1.30

47

edmh 20120402713 RBQ2

G6

A7

0.0000

edmh 20130400029 1

2.8900

H6

edmh 20120402713 RBQ2

0.0000

0.0000

0.00

48

B7

edmh 20130400029 2

0.4370

A7

edmh 20130400029 1

2.8900

3.0900

0.90

49

C7

edmh 20130400029 3

0.0207 0.5830

B7

edmh 20130400029 2

0.4370

0.4850

0.90

50

07

edmh 20130400029 3

0.0207

0.0095

2.20

edmh 20130400029 4

C7

51

E7

edmh 20130400029 RBQ

0.0000

07

edmh 20130400029 4

0.5830

0.7660

0.80

52

F7

edmh 20120407111 5 FTA

0.1160

edmh 20120407111 RBQ FTA edmh 20120407111 6

E7

edmh 20130400029 RBQ

0.0000

0.0000

0.00

53

G7

F7

edmh 20120407111 5 FTA

0.1160

0.0241

4.80

54

H7

G7

edmh 20120407111 RBQ FTA

0.0073

0.0078

0.90

55

AB

edmh 20120407111 RBK

0.0000

H7

edmh 20120407111 6

2.9700

2.1500

1.40

56

BB

edmh 20120407113 3

2.3100

0.0073 2.9700

AB

edmh 20120407111 RBK

0.0000

0.0000

0.00

57

CB

edmh 20120407113 4

0.5540

BB

edmh 20120407113 3

2.3100

0.0000

0.00

58

DB

edmh 20120407113 5

4.6300

edmh 20120407113 RBK

CB

edmh 20120407113 4

0.5540

0.0000

0.00

59

EB

DB

edmh 20120407113 5

4.6300

0.0000

0.00

60

FB

EB

edmh 20120407113 RBK

0.0000

0.0000

0.00

61

GB

lsw20130400493 RBQ

0.0000

FB

lsw20130400493 Ex2

0.0005

0.0000

0.00

62

A12

APC AR30Jan13

0.2510

GB

lsw20130400493 RBQ

0.0000

0.0000

0.00

63

B12

ANC AR30Jan13

#N/A

#N/A

0.0000

#N/A

64

HB

.

lsw20120404249 Ex5

F4

lsw20120406771 Ex1.2sp

·

26

0.00

E4

lsw20120406771

..

2.70

05

:,.,

.

0.0000

1.9500

C5

, ·

·

0.0000

5.3300

0

··

..

lsw20120406771 RBK lsw20120404249 Ex5

.

• •

·

84 C4

lsw20130400493 Ex2

0.0000 0.0005

A9

2 mn2

0.0170

0.0124

1.40

65

A9

2 mn2

0.0170

B9

RBQ mn2

0.0000

0.0000

0.00

66

B9

RBQ mn2

0.0000

C9

67

09

68

E9

69

F9

70

G9

71

H9

72

A10

73

B10

74

C10

75

010

76

E10 F10

78

77

G10

79

H10

80

A12

APC AR30Jan13

0.2510

n/a

nla

81

B12

ANC AR30Jan13

0.0000

n/a

n/a

82

AR30Jan13

Date:

Paae 2

- -

101-1l lnput

DNA+

--

---

---

--

----

-----

-

-

-

---

路 --

-

15j.11 Master Mix= 251-11 Amps

(ID+-1 0 f.d total input DNA)+

(1 01-11 extra volume for robotic pipetting) = 20J.ll total input DNA

Number of Samples to be Amped:

Normalization End Column #:

65 9

P50 Setup is used if volume is between2.0J.I and 10.9J.I P200 Setup is used if volume is over 11.0f-LI

Verify that TE volumes do not exceed 11 Q.I or 230J.!I Analyst(s):

lsw edmh

83K Plate Name: TE vol

ijl.l)

AR30Jan13 X

1.0 1.0 2.4

1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 10.0 1.0 6.4

1.0 2.7 1.0 5.5

1.0 8.8

1.0 8 .9

1.0 7.1 1.0 10.0 1.0 10.1 1.0 1.0 8.0 8 .3

1.0 1.0

Date: 30-Jan-13

-

---

- --

--

---

-

-

-

-- -

--

---

--

X

1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 3.9 1.0 1.0 1.0 1.0 1.0 1.0 4.0 1.0 3.1 1.0 6.2 1.0 1.0 1.0 1.0 1.0 1.0 1.0

83K Plate Name:

AR30Jan13

Analyst(s}: P�nP.?

lsw edmh

Date: 30-Jan-13

�,dLM0�11-?-

·

-

-

--

- ·

Ext Pit

Source

P1

A1

P1

-

--r--E--

Norm Pit

New Well

lsw201 20406772..;.;Ex3

P3

A1

0.0

0.0

81

lsw201 20406772_RBQ

P2

P3

81

0.0

0.0

P2

P1

A1

C1

lsw201 20406801 _Ex1

P3

C1

2.0

0.0

P2

P1

01

A1

lsw201 20406801 _Ex2

P3

01

3.8

0.0

P1

E1

P2

lsw201 20406801 _Ex5

A1

P3

E1

0.0

0.0

P2

A1

P2

A1

Sample Name

Well

-

·

· ·

-

-

-

-

vol (ul)

TE _vol

Reservoir

-

-

TEwell A1

P1

F1

lsw201 20406801 _Ex6

P3

F1

0.0

0.0

P1

G1

lsw201 20406801 _Ex7

P3

G1

0.0

0.0

P1

H1

lsw201 20406801 _Ex8

P2

A1

P3

H1

0.0

0.0

P2

A1

0.0

0.0

P2

A1

82

0.0

4.5

P2

A1

A2

P1

A2

lsw201 20406801 _R8Q

P3

P1

82

lsw201 20406045_Ex1

P3

P1

C2

lsw201 20406045_R8Q

P3

C2

0.0

0.0

P1

P2

E2

lsw201 1 0404779_Ex9.2

A1

P3

02

0.0

8.0

P2

A1

P1

F2

lsw201 1 0404779_R8Q

P3

E2

0.0

0.0

P1

P2

G2

lsw20090401 38 4_Ex2.3

A1

P3

F2

2.0

0.0

P2

A1

P3

G2

0.0

0.0

P2

A1

P3

H2

2.0

0.0

P2

A1

P1

H2

P1

A3

I sw20090401 384_RBK-3

lsw201 20406645_Ex6

P1

83

lsw201 20406645_RBK

P3

A3

0.0

0.0

P1

C3

lsw201 20406801 _Ex3

P2

A1

P3

83

2.0

0.0

P2

A1

P1

03

lsw201 20406801 _RBK-FTA

P3

C3

0.0

0.0

P2

P1

A1

E3

lsw201 20406801 _Ex4

P3

03

2.0

0.0

P1

P2

A1

F3

lsw201 20406801 _R8K

P3

E3

0.0

0.0

P2

A1

P1

G3

lsw201 20406045_Ex2

P3

F3

2.0

0.0

P2

A1

P1

H3

lsw201 20406045_R8K

P3

G3

0.0

0.0

P1

P2

A4

lsw201 20406771 _Ex1 .1

A1

P3

H3

2.0

0.0

P2

A1

P1

84

lsw201 20406771 _R8K

P3

A4

0.0

0.0

P1

C4

lsw201 20404249_Ex5

P2

A1

P3

84

2.0

0.0

P1

04

P2

lsw201 20404249_R8K

A1

P3

C4

0.0

0.0

P2

P1

E4

A1

lsw201 20406578_Ex1

P3

04

2.0

0.0

P2

A1 A1

P1

F4

lsw201 20406578_R8K

P3

E4

0.0

0.0

P2

P1

G4

lsw201 20406998 _Ex1

P3

F4

2.0

0.0

P2

P1

A1

H4

lsw201 20406998_R8K

P3

G4

0.0

0.0

P1

85

P2

lsw201 20407000_R8K

A1

P3

H4

0.0

0.0

P1

C5

P2

lsw201 20406771 _Ex1 .2sp

A1

P3

A5

2.0

0.0

P2

A1

P1

05

lsw201 20406771 _Ex1 .2epi

P3

85

2.0

0.0

P1

P2

E5

lsw201 20406771 _R8Qsp

A1

P3

C5

0.0

0.0

P2

A1

P1

F5

lsw201 20406771 _R8Qepi

P3

05

0.0

P1

0.0

G5

edmh_201 204071 1 1 _1

P2

A1

P3

E5

0.0

0.0

P1

P2

H5

edmh_201 204071 1 1 _2

A1

P3

F5

0.0

0.0

P1

P2

A6

A1

edmh_201 204071 1 1 _3

P3

G5

0.0

0.0

P2

A1

P1

86

edmh_201 204071 1 1 _4

P3

H5

0.0

2.3

P1

C6

P2

edmh_201 204071 1 1 _R8Q

A1

P3

A6

0.0

0.0

P2

A1

P1

06

edmh_201 204071 1 3_1

P3

86

0.0

0.0

P1

E6

edmh_201 204071 1 3_2

P2

A1

P3

C6

0.0

0.0

P1

P2

F6

edmh_201 204071 1 3_R8Q

A1

P3

06

0.0

0.0

P1

G6

edmh_201 2040271 3_ 4

P2

P3

A1

E6

4.5

0.0

P2

A1

P1

H6

edmh_201 2040271 3_RBQ2

P3

F6

0.0

0.0

P1

P2

A7

edmh_201 30400029_1

A1

P3

G6

2.0

0.0

P2

A1

3.4

00 .

P2

0.0

0.0

P2

H6

A1

P1

87

edmh_201 30400029_2

P3

P1

C7

edmh_201 30400029_3

P3

A7

P1

07

edmh_201 30400029_4

P3

87

2.6

P1

0.0

E7

P2

edmh_201 30400029_RBQ

A1

P3

C7

0.0

0.0

P2

A1

A1

P1

F7

edmh_201 204071 1 1 _5_FTA

P3

07

0.0

G7

7.1

P1

P2

edmh_20120407111_RBQ_FTA

A1

P3

E7

0.0

0.0

P2

A1

P1

H7

edmh_201 204071 1 1 _6

P3

F7

2.0

0.0

P1

AS

P2

edmh_201 204071 1 1 _RBK

A1

P3

G7

0.0

0.0

P2

A

D'?f1

AR �n l!ln1�

AR 30Jan1 3

flV?(

v

...

-

Ext Pit

Source

Sample Name

Well

P1

88

-- - --

edmh_201 204071 1 3_3

-

---

-

·

-

·

--

Norm Pit

New Well

P3

H7

-·-

-

-

Template vol (ul) 2.0

TE_vol 0.0

TE Reservoir P2

TEwell A1

P1

CB

edmh_201 204071 1 3_4

P3

AB

2.7

0.0

P2

A1

P1

DB

edmh_201 204071 1 3_5

P3

88

2.0

0.0

P2

A1

P1

E8

edmh_201 204071 1 3_RBK

P3

P1

F8

lsw201 30400493_Ex2

P3

CB

08

0.0

0.0

P2

A1

0.0

0.0

P2

A1

P1

GB

lsw201 30400493_RBQ

P3

E8

0.0

0.0

P2

A1

P1

A1 2

APC_AR30Jan 1 3

P3

F8

6.0

0.0

P2

A1

ANC_AR30J an 1 3

P1

81 2

P1

empty

P1

A9

P1

89

P3

GB

0.0

0.0

P2

A1

P3

H8

0.0

0.0

P2

A1

jtm201 20404427_2_mn2

P3

A9

0.0

0.0

P2

A1

jtm201 20404427_RBQ_mn2

P3

89

0.0

0.0

P2

A1

P?n AR �n.l::1�

AR 30Jan1 3

Ext Pit

-

·

-

- · --

------ - �

Sample Name

Well

-

-

--

·

-

-

--

Norm Pit

New Well

· Template-

Reservoir

-

---

TE well A1

P2

20.0

P2

A1

45.5

P2

A1

1 6.3

P2

0.0

P2

A1

A1

P1

81

1sw201 20406772_R8Q

P3

81

P1

C1

lsw201 2040680 1 _Ex1

P3

C1

0.0

P1

01

lsw201 20406801 _Ex2

P3

01

0.0

P1

E1

lsw201 20406801 _Ex5

P3

E1

20.0

P1

F1

lsw201 20406801 _Ex6

P3

F1

20.0

P1

G1

lsw201 20406801 _Ex7

P3

G1

P1

H1

A2

lsw201 20406801 _Ex8

P3

H1

tsw201 20406801 _R8Q

P3

82

lsw201 20406045_Ex1

P3

P1

C2

lsw201 20406045_R8Q

P3

P1

E2

lsw201 1 0404 779_Ex9.2

P1

F2

lsw201 1 0404779_R8Q

P1

G2

lsw20090401 38 4_Ex2.3

P1

G2

lsw20090401 38 4_Ex2.3

P3

...- P1

H2

lsw20090401 38 4_R8K-3

P3

P1

A3

lsw201 20406645_Ex6

P3

H2

P1

A3

lsw201 20406645_Ex6

P3

P1

83

lsw201 20406645_RBK

P1

C3

P1

-

-

·

A1

0.0

P2

A1

0.0

P2

A1

0.0

P2

A1

20.0

0.0

P2

A1

1 5.5

0.0

P2

A1

20.0 20.0

82

-

0.0

P3

P1

TE

0.0

lsw201 20406772_Ex3

A2

-

TE _vol

20.0

A1

·

-

vol (ul)

P1

P1

..

. - -

-

Source

-

C2

20.0

0.0

02

0.0

P2 P2

A1

12 0

E2

20.0

0.0

P2

A1

0.0

98.9

P2

A1

F2

0.0

98.9

P2

A1

G2

20.0

0.0

P2

A1

0.0

63.0

P2

A1

H2

0.0

63.0

P2

A1

P3

A3

20.0

0.0

P2

A1

lsw201 20406801 _ Ex3

P3

83

0.0

52.4

P2

A1

03

lsw201 20406801 _R8K-FTA

P3

C3

20.0

0.0

P2

A1

P1

E3

lsw201 20406801 _Ex4

P3

03

0.0

1 07.9

P2

A1

P1

F3

lsw201 20406801 _RBK

20.0

0.0

P2

A1

0.0

8 7.2

P2

A1

P2

A1

P2

A1

P2

A1

G3

P1

P3

P3 P3

.

F2

P3

E3

lsw201 20406045_Ex2

P3

F3

A1

P1

G3

lsw201 20406045_Ex2

P3

F3

0.0

87.2

P1

H3

lsw201 20406045_RBK

P3

G3

20.0

0.0

P1

A4

lsw201 20406771 _Ex1 .1

P3

H3

0.0

88.4

P1

A4

lsw201 20406771 _Ex1 .1

P3

H3

0.0

88.4

P2

A1

P1

84

lsw201 20406771 _RBK

P3

A4

20.0

0.0

P2

A1

P1

C4

lsw201 20404249_Ex5

P3

84

0.0

70.1

P2

A1

P1

C4

lsw201 20404249_Ex5

P2

A1

P1

04

, P1

84

0.0

P3

C4

20.0

0.0

P2

A1

E4

lsw 201 20404249_RBK

P3

70.1

lsw201 20406578_Ex1

P3

04

0.0

99.0

P2

A1

P1

E4

lsw201 20406578_Ex1

P3

04

0.0

99.0

P2

A1

P1

F4

lsw201 20406578 _RBK

P3

E4

20.0

0.0

P2

A1

P1

G4

lsw201 20406998_Ex1

P3

F4

0.0

1 00.0

P2

A1

P1

G4

lsw201 20406998_Ex1

P3

F4

0.0

1 00.0

P2

A1

P1

H4

lsw201 20406998 _RBK

P3

G4

20.0

0.0

P2

A1

P1

85

lsw201 20407000_RBK

P3

H4

20.0

0.0

P2

A1

P1

C5

lsw201 20406771 _Ex1 .2sp

P3

0.0

79.4

P2

A1

P1

C5

lsw201 20406771 _Ex1 .2sp

P3

0.0

P2

A1

P1

05

lsw201 20406771 _Ex1 .2epi

P3

0.0

79.4 81 .7

P2

A1

P1

05

lsw201 20406771 _Ex1 .2epi

P3

85

0.0

81 .7

P2

A1

P1

E5

lsw201 20406771 _RBQsp

P3

C5

20.0

0.0

P2

A1

P1

F5

lsw201 20406771 _RBQepi

P3

05

20.0

0.0

P2

A1

P1

G5

edmh_201 204071 1 1 _1

P:3

E5

20.0

0.0

P2

A1

P1

H5

edmh_201 204071 1 1 _2

P3

0.0

P2

A1

P1

A6

edmh_201 204071 1 1 _3

P-3

F5 G5

20.0 20.0

0.0

P2

A1

P1

86

edmh_201 204071 1 1 _ 4

. P3

H5

1 7.7

0.0

P2

A1

P1

C6

edmh_201 204071 1 1_RBQ

P3

A6

20.0

0.0

P2

A1

P1

06

edmh_201 204071 1 3_1

P3

P1

E6

edmh_201 204071 1 3_2

P1

F6

edmh_201 204071 1 3_R8Q

P1

G6

edmh_201 2040271 3_4

P1

H6

edmh_201 2040271 3_RBQ 2

n.-,nn

AD ':U11-:n1'<

AS AS

85

86

20.0

0.0

P2

A1

C6

20.0

0.0

P2

A1

P3

06

20.0

0.0

P2

A1

P3

E6

0.0

1 5.5

P2

A1

P3

F6

20.0

0.0

P2

A

P3

AR 30Jan1 3

iJl

v

·

· -----

Ext Pit

Source Well

Sample Name

-

-

- -

-

Norm Pit

New Welt

----

-

----

Template

)

vel {ul

-

-

TE_vot

-

--

-

-

TE Reservoir

-

- -

· - - --

TEwell

P1

A7

edmh_201 30400029_1

P3

G6

0.0

75.1

P2

A1

P1

87

edmh_201 30400029_2

P3

H6

0.0

1 6.6

P2

A1

P1

C7

edmh_201 30400029_3

P3

A7

20.0

0.0

P2

A1

P1

07

edmh_201 30400029_4

P3

87

0.0

1 7.4

P2

A1

P1

E7

edmh_201 30400029_R8Q

P3

C7

20.0

0.0

P2

A1

P1

F7 G7

edmh_201 204071 1 1 _5_FTA

P3

0.0

edmh_20120407111_RBQ_FTA

P3

07 E7

1 2.9

P1

20.0

0.0

P2

P2

A1 A1

P1

H7

edmh_201 204071 1 1 _6

P3

F7

0.0

77.2

P2

A1

P1

AS

edmh_201 204071 1 1 _RBK

P3

G7

20.0

0.0

P2

A1

P1

88

edmh_201 204071 1 3_3

P3

H7

0.0

59.6

P2

A1

P1

C8

edmh_201 204071 1 3_4

P3

AS

0.0

1 7.3

P2

A1 A1

P1

08

edmh_201 204071 1 3_5

P3

88

0.0

60.8

P2

P1

08

edmh_201 204071 1 3_5

P3

88

0.0

60.8

P2

A1

P1

E8

edmh_201 204071 1 3_R8K

P3

C8

20.0

0.0

P2

A1

P1

F8

lsw201 30400493_Ex2

P3

08

20.0

0.0

P2

A1

P1

G8

lsw201 30400493_RBQ

P3

EB

20.0

0.0

P2

A1

0.0

1 4.0

P2

A1

0.0

P2

A1

P1

A1 2

APC_AR30Jan1 3

P3

F8

P1

81 2

ANC_AR30Jan1 3

P3

GB

20.0 .

P1

empty

P3

H8

0.0

0.0

P2

A1

P1

A9

jtm201 20404427_2_mn2

P3

A9

·0.0

0.0

P2

A1

P1

89

jtm201 20404427_RBQ_mn2

P3

89

0.0

0.0

P2

A1

yJ-(f

AR �n.l�n1�

- --

-

--

·

Florida Department of Law Enforcement

(FDLE

Biology" Section

3130x/ Plate Record Biomek 2000 Operator: Analyst(s): Date: Plate Record Name: Biomek 2000 Method:

Instrument ID: Capillary Array Lot: Array Count or Date: POP4 Lot: Buffer Lot:

�io·s� �W�if� A1 A2 81 82 C1 C2

01

02 E1 E2 F1 F2 G1 G2 H1 H2

A3 A4 83 84 C3 C4 03 04 E3 E4 F3 F4 G3 G4 H3 H4

A5 AS 85 66 C5 05 06 E5

E6 F5 F6 G5 G6 H5 HS

../=Used V- "'1-' I

1--..J

10+ 10+ 10+ 10+ 10+ 10+

10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+

10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+

10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+

3130xiB 32A06704 7 1209121 1209410

10+ Ladder lsw20120406772 Ex3 lsw20120406772 RBO lsw20120406801 Ex1 lsw20120406801 Ex2 lsw20120406801 Ex5 lsw20120406801 Ex6 lsw20120406801 Ex7 lsw20120406801 Ex8 lsw20120406801 RBQ lsw20120406045 Ex1 lsw20120406045 RBQ lsw20110404779 Ex9 .2 lsw20110404779 RBQ lsw20090401384 Ex2.3 lsw20090401384 RBK-3 10+ Ladder lsw20120406645 Ex6 lsw20120406645 RBK lsw20120406801 Ex3 lsw20120406801 RBK-FTA lsw20120406801 Ex4 lsw20120406801 RBK lsw20120406045 Ex2 lsw20120406045 RBK lsw20120406771 Ex1.1 lsw20120406771 RBK lsw20120404249 ExS lsW20120404249 RBK lsw20120406578 Ex1 lsw20120406578 RBK lsw20120406998 Ex1 10+ Ladder lsw20120406998 RBK lsw20120407000 RBK lsw20120406771 lsw20120406771 Ex1.2epi lsw20120406771 lsw20120406771 RBQepi edmh 20120407111 1 edmh 20120407111 2 edmh 20120407111 3 edmh 20120407111 4 edmh 20120407111 RBQ edmh 20120407113 1 edmh 20120407113 2 edmh 20120407113 RBQ edmh 20120402713 4

Pace 1

rf

lsw edmh

. };_,· :�---�--

31-Jan-13

AR 30Jan13 61-75 ID+ Ladder Lot:

1205041

Yfiler Ladder Lot: Formamide Lot:

GS 600 Lot: Denature:

1202160 1207049 TC#3@ 95d

!?

A7 AS 87 88 C7 CB 07 DB E7 EB F7 FB G7 GB H7 HB A9 A10 89 810 C9 C10 09 010 E9 E10 F9 F10 G9 G10 H9 H10 A11 A12 811 812 C11 C12 011 012 E11 E12 F11 F12 G11 G12 H11 H12

./=Used

ID+ ID+ ID+ ID+ ID+ ID+ ID+ ID+ ID+ ID+ ID+ 10+ 10+ ID+ 10+ 10+

ID+ Ladder edmh 20120402713 RBQ2 edmh 20130400029 1 edmh 20130400029 2 edmh 20130400029 3 edmh 20130400029 4 edmh 20130400029 RBQ edmh 20120407111 5 FTA edmh 20120407111 RBQ FTA edmh 20120407111 6 edmh 20120407111 RBK edmh 20120407113 3 edmh 20120407113 4 edmh 20120407113 5 edmh 20120407113 RBK lsw20130400493 Ex2 10+ 10+ Ladder ID+ lsw20130400493 RBQ 10+ APC AR30Jan13 ID+ ANC AR30Jan13 10+ no 10+ 2 mn2 10+ RBQ mn2 10+ no 10+ no sample 10+ no sample 10+ no 10+ no sample 10+ no sample 10+ no sample 10+ no sample 10+ no sample

ID+ 10+ 10+ 10+ 10+ ID+ 10+ 10+ 10+ 10+ 10+ ID+ ID+ 10+ 10+ ID+

Paae 2

plexor plexor

Florida Department of Law Enforcement Biology Section RUN ANALYSIS FORM Analyst

fW.

Run Folder.

Case Number(s) or Sample Set

S

!r.:o:> Location:

!-'\.

t.SN

GS600 present and correc tly labeled

All ladders have correct allelic calls

Alleles above threshold/below

All controls have correct results (Positive/Negative/Blanks)

max rfu,

peak heights within ratio

Comments:

� 0 \� bL-\ 6 �OL\S

\

Date

Original in first case listed

Applied B1osystems

31308 AR 30Jan131sw

GeneMapper10 v3.2.1

Name

File

A02 002 lsw20120406772 Ex3 90

150

Panel

Ex3

0 1.fsa

270

210

Plus Panels v1

0 330

390

90 60

. A02 路ooz lsw20120406772 Ex3 90

Ex3

o 1.fsa 150

210

0

Plus Panels v1

.

270

.

330

390

90 60

A02 002 lsw20120406772 Ex3 90

0 1.fsa 150

lsw20120406772 Ex3 210

0 270

ldentifiler Plus Panels v1 330

390

90 60 30

Ao2 002 lsw20120406772 Ex3 90

801 003 lsw20120406772 RBQ 90

0 1.fsa 150

lsw20120406772 Ex3 210

150

270

RBQ

0 1.fsa

0

210

ldentifiler Plus Panels v1 330

0 270

390

Plus Panels v1 330

390

90 60 30

Mon Feb 11,2013 01:57PM, EST

Printed by: lsw

Page 1 of 2

Applied B1osystems

31308 AR 30Jan13 lsw

GeneMapper ID v3.2.1

Name

File

801 003 lsw20120406772 RBQ

0 1.fsa 150

90

Panel

lsw20120406772 RBQ 210

0 270

ldentifiler Plus Panels v1 330

390

90 60

801 003 lsw20120406772 RBQ 路o 1.fsa 150

90

lsw20120406772 RBQ 210

0 270

ldentifiler Plus Panels v1 330

390

90 60 30

801 003 lsw20120406772 RBQ 90

o

1.fsa

lsw20120406772 RBQ

150

210

0 270

ldentifiler Plus Panels v1 330

390

90 60

Mon Feb 11,2013 01:57PM, EST

Printed by: lsw

Page 2 of 2

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Page 1 of 1

·FDLE

Florida Department of

Jacksonville Regional Operations Center

Rick Scott, GJvernor

Law Enforcement

921 N. Davis Street, Building E Jacksonville, Florida 32209-683 8

Pam Bond� Attorney General

Gerald M. Bailey

1-800-226-6481

Adam Putnam, Commissioner of Agriculture

Commissioner

www.fdle.s tate.fl.us

Jeff Atwater, Chief Financial Officer

LABORATORY REPORT February12,2013 Sheriff John H. Rutherford

TO:

FDLE NUMBER:

Jacksonville Sheriffs Office

SUBMISSION:

501 East Bay Street

AGENCY NUMBE R:

Jacksonville, FL 32202-2975 ATTN:

M. A. Musser61139

VICTIM (S):

Jordan Russell Davis

SUBJE CT(S):

Michael David Dunn

0 FFENSE(S):

20120406772 1 and2

12-0853093

SUBPOENAS PERTAINING TO THIS CASE SHOULD REFE R TO THE FDLE NUMBER.

Death Investigation

L. SukHan Warf

Duval County 11/23/2012

Crime Laboratory Analyst Biology Section

RE FE RENCE :

This report references evidence submited to the Florida Department of Law Enforcement on December06,

2012 and on December18,2012 by Maurice Lawrence. This report may contain conclusions, opinions, and/ or interpretations made by the author. E VIDENCE : FDLE

Agency

Item#

E xhibit# 1-9

3 8

9 10

6-2 6-3 6-4

Description

DNA swab from shell casings at scene

DNA swabs from shell casings recovered from suspect vehicle DNA swab from top of magazine recovered from suspect vehicle DNA swab from live rounds recovered from suspect vehicle

RE SULTS:

STR DNA analysis was performed on a sample from Exhibit 3 utilizing the AmpF lSTR Identif.tler Plus PCR Amplification Kit.

No DNA results were obtained from Exhibit 3 . STR DNA analysis was not performed on Exhibits 8,9 and10 .

1111111111111111111111111111111111111111

Service

Page 1 of2

Integrity

Respect

Quality

FDLE NUMBER: 20120406772 Submissions: 1 and 2 REMARKS: ·

·· · · ·

The·submited evidence· is available-Jor retrieval orreturatthe earliest opportunity; Thls evidence ·includes DNA extracts. It is recommended that all DNA extracts be stored frozen. Abbreviations that may appear in this report are: CODIS Combined DNA Index System; DNA

deoxy ri bonuc leic acid; PCRPolymerase Chain Reac tion; STR short tandem repeat; Y STR male spe cific short tandem repeat; FBI F e de ral Bureau ofInvestigation; and JFS Journal ofForensi c Sciences. -

Questions regarding this report should be addressed to: jrocbiology@fdle. state.fl.us.

Page� of2

ASCLDILAB-International

STATEMENT OF QUALIFICATIONS

Name

Date

Lucretia SukHan Warf

of Law Enforcement I Jacksonville

Florida

Job Title

sno12o13 Center

Crime

Indicate all disciplines in which you do casework:

D D D D D

Drug Chemistry Firearms/Tool marks Trace Evidence Latent Prints

Digital & Multimedia Evidence

D IX] D D

Toxicology Biology Questioned Documents Crime Scene

L ist all category(ies) of testing in which you do casework:

and DNA. Breath Alcohol Calibration Categories

D

D

Toxicology- Breath Alcohol Measuring Instruments (The work of the laboratory MUST include calibration certificatesdo not check the box if work is limited to breath/alcohol testing)

- Breath Alcohol Calibration Reference Material

Education: List all higher academic institutions attended (list high school only if no college degree has been attained) Institution

Dates Attended

ofNorth Florida

8/1998 - 5/2002

of Florida

5/2003 - 1 2/2005

Major

Degree

B. S.

Forensic DNA & Serology

M.S.

Other Training: List continuing education, workshops, in-service and other formal training received. Please include the course title, source and date of the training.

University of North Florida, Biochemisty FDLE Forensic Technologist Serology and DNA training program FDLE Crime Laboratory Analyst Serology and DNA training program Paternity Index DNA Statistics, SAFS, MAFS, MAAFS, CAS Joint meeting Applied Biosystems GeneMapper ID Workshop, SAFS, MAFS, MAAFS, CAS Joint meeting GeneMapper ID Training, FDLE 3130xl Genetic Analyzer training, FDLE Statistics in the Courtroom, 5th Annual Advanced DNA Technology Workshop, The Bode Technology Group, Inc. Beckman Biomek 3000 and 2000 rpbotics training with Promega's DNA IQ extraction system, FDLE HID University 3130xl and Genemapper ID training, Applied Biosystems FBI DNA Auditor Workshop DNA Identifications for Missing Persons and Disasters SAFS, MAFS, MAAFS, SWAFS Joint meeting YSTRs: Science and Statistics SAFS, MAFS, MAAFS, SWAFS Joint meeting ASCLD/LAB International Preparation Course, ASCLD/LAB Plexor HY

and PowerPlex Y training, Promega

ASCLD/LAB-lnternational Statement of Qualifications

Approval Date: August 3, 2012 Approved By: Executive Director

Page 1 of2

Effective Date: August 3, 2012 AL-PD-3018-Ver 3.0

Plexor HY quantitation training, FDLE

Yfiler training, FDLE

Identifiler Plus training, FDLE Courtroom Experience: List the discipline/category(ies) of testing in which you have qualified to testify and indicate over what period of time and approximately how many times you have testified in each.

as

an expert witness

Serology (since 2005):25 DNA (since 2005) :35 Professional Affiliations: List any professional organizations of which you are or have been a member. Indicate any offices or other positions held and the date(s) of these activities.

Employment History: List all scientific or technical positions held, particularly those related to forensic science. List current position first. Be sure to indicate employer and give a brief summary of principal duties and tenure in each position.

Job Title

Tenure

Crime

2/2005 -

FDLE Provide a brief

Job Title

duties:

of

STR-DNA

evidence

of evidence and courtroom

Tenure

Crime

Crime

FDLE of

Provide a brief In

duties : of evidence and courtroom

to learn STR-DNA

Job Title

Tenure

Forensic

8/2002 - 5/2004

FDLE of

Provide a brief

duties :

General lab support, instrument maintenance and operation, analyst support, sweep cases for trace evidence, general serology evidence

Job Title

Tenure

Volunteer

3/2002 - 8/2002

FDLE duties:

Provide a brief description of Evidence

office

and

Tenure

Job Title Provide a brief

of

duties :

Other Qualifications: List below any scientific publication and/or presentation you have authored o r co-authored, research in which you are or have been involved, academic or other teaching positions you have held, and any other information which you consider relevant to your qualification as a forensic scientist. (Use additional sheets if necessary. )

FDLE Plexor HY and Y-filer internal validation team

ASCLD/LAB-lnternational Statement of Qualifications

Approval Date: August 3 , 20 1 2

Approved By: Executive D irector

Page 2 of 2

Effec-tive Date: August 3, 20 1 2 AL-PD-3 0 1 8-Ver 3 .0

tj_

Form

Full Member

IF Associate Member, Name of Full Member Sponsor

Badge Name

tY'-:C. \ ·L-£.

Drivers License No . Newsletter by Email? Email Address :

.

.;:' ) Yes

4- -1

D 6 <;

.J

-4 ... Vice President.

No

I

NRA Membership Number

�- Cbc"'­

.

Treasurer

Executive Officer

Sign me up for the NRA

I wil email my NRA number

Initiation F ee Yearly Dues NRA Fee Total

I certify that I

1, () 6 �s

?>�1

of good repute; that I am not a member of any organization or group having as its purpose the overthrow or

disruption by force or viol ence the Gov ernment of the United States of America or any of the poli tical subdivisions; that I have am

never been convicted of a crime of violence; that if admitted to membership, I will fulfill the obligations of good sportsmanship and good citizenship . I hereby attest the above statements are true, and any falsehood will deprive me of membership. I understand that I must remain an NRA member for the duration of my club membership. I also understand that by signing this application I am consenting to a possible background check.

Applicant Signature

FOR CLUB USE ONLY Orientation Attended:

Date Orientation Lead Signature

Page �

Revised 1 0/2 1 109

RELEASE AND HOLD HARMLESS AGREEMENT In consideration for participating in club activity sponsored or organized by the Port Malabar Rifle and Pistol Clu b, Inc., the undersigned Participant HEREBY AGREES TO WAIVE, RELEASE, DISCHARGE, AND HOLD HARMLESS the Port Malabar Rifle and Pistol Club, Inc . from any and all claims for damages for death , personal injury, or property damage which they may have or which they may hereafter accrue as a result of participation in any Port Malab ar Rifle and Pistol Club, Inc. program or related activity and events including, but not limited to unorganized practice, organized practice, training and instruction and organized shooting events. By signing this Release and Hold Harmless Agreement, the undersigned recognizes that the sport of rifle, pistol, and shotgun shooting or related activities present certain inherent dangers that cannot be eliminated even by the exercise of reasonable care. As such, the undersigned Participant hereby expressly assumes the risk that is inherent to the sport of rifle, pistol, and shotgun shooting. As a requisite condition to the Participant being allowed to attend and participate in any Port Malabar Rifle and Pistol Club, Inc. sponsored event or trip, the undersigned Participant hereby acknowledges that the State of F lorida has the most significant relationship with the p arties to this Agreement rather then the destination state or providence. As such, the parties hereto expressly agree to F lorida

as

the Choice of Law and Choice of Forum to any claim, suit, or controversy that m ay arise by and between the

p arties or any of its related affiliates, and further agree that any action, suit, or claim made by the Particip ant

must

be brought in the

State of Florida as the proper Venue having exclusive subj ect matter jurisdiction to -any such claim, demand, or suit. To effectuate this expressed Choice of Law, Choice of Forum and V enue provision, the undersigned hereby agrees that the state or providence where the injury or cause of action arose shall hereto .

not have

either subj ect matter jurisdiction or

in personam jurisdiction

over the p arties

By signing this Release and Hold Harmless Agreement, the undersigned Participant assumes the risk inherent to rifle, pistol, and shotgun shooting, and thereby consents to the Participant's p articipation in Port Malabar Rifle and Pistol Club, Inc. programs and related activities, and further acknowledge that he/she understands that all risks, whether known or unknown, are expressly assumed by the undersigned, and that all claims, whether known or unknown, are expressly waived in advance. In addition to the foregoing, the undersigned Particip ant hereby expressly agrees and recognizes that the Port Malabar Rifle and Pistol Club, Inc., through its individual Match Directors, Match Chairmen, Range Officers, judges and referees, officers, directors, agents, individuals or entities perform

volunteer services,

and as such, said entities and/or individuals are immune from

liability as volunteer leaders, assistants, and organizers.

Arbitration :

I n further consideration for being allowed t o participate i n any activities sponsored b y the Port Malabar Rifle and Pistol Club, Inc. the undersigned P articipant recognizes and agrees that this contract involves interstate commerce which is necessarily

ยง 2), and therefore, pursuant to the Federal "Arbitration Act (FAA) as well as (Florida Statutes ยง 682.02) hereby agree to submit to binding arbitration any and all claims, demands,

controlled by the Federal Arbitration Act (9 USC the Florida Arbitration Act

. suits, or other disputes that may arise out of this contract, or that may be presented by or on beh alf of the Participant for resolution

under the Commercial Rules of the American Arbitration Association with the initial filing fees thereof to be paid by the party filing the Demand for Arbitration subject to the schedule of fees then in effect at the time of the filing of a Demand with the American Arbitration Association. I HAVE READ THE FOREGOING TERMS AND CONDITIONS AND AGREE TO BE BOUND BY ALL OF THE CONDITIO

S S ET FORTH ABOVE.

Page 1

Revised 1 0/2 1/09

Detective Detective Detective Detective

T. A. Stapp #7725 M . A. M usser #61 1 39 (Lead Detective) E. M. Cayenne #6060 T. D. O l iver #7779

WITNESSES INTERVIEWED BY DETECTIVE CAYENNE

D ETAILS OF I NVESTIGATION F riday/1 1 -23-1 2/2000 hours : Detective Cayenne received a phone call from Sergeant Paul advising our team was bei n g called to 8251 Southside Boulevard (Gate Gas Station) in reference to a shooting in the parking lot. Sergeant Pau l advised that when we are done with our other crime scene we could meet at the Gate Gas Statio n . Note: Let i t be n oted that Detectives Stapp and Cayenne was handling another death investigation a n d never made it to the crime scene. F riday/1 1 -23-1 2/231 0 hours: Detective Cayenne spoke with Sergeant Paul and advised him that Detectives Cayenne and Sta pp were done with our death i nvestigation where did he need us to respond. Sergeant Pau l advised Detective Cayenne to respond to t h e Homicide Office. F riday/1 1 -23-1 2/230 hou rs: Detective Cayenne arrived at the Homicide Office. Tuesday/1 1 -27 -1 2/1 805 hours : Detectives Caye n ne and Stapp responded to the strip mall located at 8221 Southside Boulevard to cond uct a canvass of the businesses. Detective Cayenne spoke with owners or employees of the following businesses: U n it 3- Kalu by's Dance Club advised they had video surveillance i nside the business and outside on the back side. There was no video outside the front of the business. U n it 5 - The Loop Restaurant advised the business has s u rveillance inside and views outside the rear of the business. There was no video outside the front of the business. U n it 1 1 - Tropical Smoothie advised the business has surveillance inside and views outside the rear of the business. There was no video outside the front of the business. Detective Cayenne spoke with employee who stated he was working on the n i g ht of the shooting. He advised he d id not hear any g u nshots. remem bered a police car had pulled over a veh icle in the Publix parking lot. The next th ing he remem bered were m u ltiple police cars responding to the scene. He did not learn of the shooting until later that night. Detective Cayenne spoke with witness the incident. did not hea r any g u nshots.

SUP

Page 2 of 4

Date Printed: 1 013/2013

who was working on the night of

J O H N H . RUTH E RF O RD, S H E RI F F

2012 853093-9

SUP

Case Cleared CLEARED

BY ARREST (DETECTIVE)

CRIME ANAL YSIS Aggravated Assault/Murder: ARG U M ENT Type of Weapon: HAN DGUN ( DISCHARGED) Forced Entry: NOT APPLICABLE Structure Occupancy Code: NOT APPLICABLE Number ofPremises Entered: Location Type: Gas/Service Station(business that services vehicles) Incident Occurred Outside th is Location Incident Occurred In the Parking Lot at this Location: Number ofArrested: 1 Number of Vehicles Recovered: School Name: School Number

Yes

MISCELLANEOUS: Is Offense Related to Domestic Violence: No Ifyes, were Children under 18 Present: No IfNo is it Domestic Related: NO Is there additional information included on a continuation report: No Are there other Pertinent Reports: Yes In your opinion is there sign(ficant reason to believe that the crime can be solved by a patrolfollow-up investigation? No Neighborhood Canvass Conducted: Yes Case Information Card Left With: N.A. If Other (Name Address): Address: Apt./Lot #: Sub-Sector: City: State: Zip: Taz: Crossstreet: Home Phone # Bus. Phone # Ext. Cell Phone # Cell Phone Provider E-mail Is Vagrant?

NO

Investigative Time: #1 0 Hours: 1 0 Minutes: 0

Cost Amount:

Detective Called To the Scene: M .A . M USSER

Evidence Technician Called to the Scene: Reporting Officers:

SUP

#61 1 39

#0

E.M.CAYEN N E

Approving Supervisor M .A. PAU L Division: DETECTIVE Unit:

Page 4 of 4

$482.40

( 1 [ 1 ID #6060 1D #0 ID #6097

NA:

Not(fied: [ X

NA:

Assigned By HQ: [ X

1

8/1 5/201 3

1 1 3 :05

HOMICIDE

Date Printed: 1 01312013

J O H N H . RUTH E RF O R D , S H E RI F F

2012 853093-9

SUP

at the B u rger King located near the i ntersection of Beach Boulevard and St. Johns Bluff. Th is report documents all ind ivid ual investigative efforts in this case that were conducted by Detective Oliver. Refer to Detective M usser's report for more detai ls of the investigation.

Clearance Status: CLEARED BY ARREST Clearance Code: Case Cleared C LEARED BY ARREST (DETECTIVE)

ADULT Date case was cleared: 1 1 /24/201 2

Number ofCases Cleared

CRIME ANAL YSIS Aggravated Assault/Murder: ARG U MENT Type of Weapon: HANDGUN (DISCHARGED) Forced Entry: NOT APPLICABLE Structure Occupancy Code: NOT APPLICABLE Number ofPremises Entered: Location Type: Gas/Service Station(business that services veh icles) Incident Occurred Outside th is Location Incident Occurred In the Parhng Lot at this Location: Number of Vehicles Recovered: Number ofArrested: 1 School Name: School Number

Yes

MISCELLANEOUS: Is Offense Related to Domestic Violence: No Jfyes, were Children under 18 Present: No IfNo is it Domestic Related: NO Is there additional information included on a continuation report: No Are there other Pertinent Reports: Yes In your opinion is there significant reason to believe that the crime can be solved by a patrolfollow-up investigation? No Case Information Card Left With: N .A. Neighborhood Canvass Conducted: Yes If Other (Name Address): Address: Apt./Lot #: Sub-Sector: City: State: Zip: Taz: Crossstreet: Home Phone # Bus. Phone # Ext. Cell Phone # Cell Phone Provider E-mail Is Vagrant?

NO

Investigative Time: #1 1 Hours: 0 Minutes: 0

Cost Amount:

Detective Called To the Scene: M .A.M USSER Evidence Technician Called to the Scene: Reporting Officers:

T. D. 0 LIVER

Approving Supervisor M .A. PAUL Division: DETECTIVE Unit:

SUP

Page 3 of 3

#61 1 39

#0

NA:

[ 1 1 #7779 #0 #6097

NA: [ ID ID ID

Notified:

[X1

AssignedBy HQ: [

X1

8/1 9/201 3

1 4:23

HOMICIDE

Date Printed: 1 0/312013

J O H N H . RUTH E RF O RD , S H E RI F F

2012 853093 1 0

SUP

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enough filler, on to the real news. Thought of you yesterday after all of this and said man Mike probably really enj oy this

article and the talk back section.

e Are Watching You Act, propo sed by

Democratic Massachuset Rep . Michael Capuano and

Rep lican North Carolina Rep. Walter Jones, will keep corporations from using digiW vide<>.t" recor ers (DVRs) to collect and share your personal information.

Kine info

Polygon reports. The bill would require the consumer to provide prior consent before

ation is collected, and the operator of the

data s collected, said a press release on the House's website. Infr

info offer

would have to provide access to how the

installed in the DVRs would be used to collect the surveillance ed cameras and would be forc�d to ation. If not given a consumer ' s permission to be filmed, the

other option that does not film their consumers but that is "otherwise identical in all aspects."

If a c nsumer does allow the information to be collected, a bright red message that reads "WE ARE WAT HING YOU" must be flashed across the screen at all times while recording. The

ouse 's press release explains the teci.Jx.• companies ' interest in this technology: Essentially, they

want to better and more quickly figure out what to sell you- a way to "super-target ads," Capuano said .

"If s meone is watching a favorite program while cleaning the kitchen, ads for products that will make appli ces shine may show up. If a couple is talking about where to dine over the weekend, or enjoying

ac

chy snack while watching the Stanley Cup finals, their television may serve up food suggestions.

The atent application even goes so far as to suggest that if a couple is fighting, an adverti semen.tt"'

asso iated with relationship counseling might

be selected for them," it said.

Jone and Capuano both offered quotes in their press release.

In li ht of the government's "unfortunate history of secretly collecting private citizens ' information we must ensure

from Jone said. Cap and

that safeguards are in place to protect Americans ' rights,"

o stressed that though "this may sound preposterous ," it is "neither a j oke nor

an

exaggeration,"

ged consumers to consider the impact this sort of information- sharing will have on their personal about what you do in the privacy of your own home and then think about how· you would feel

g that information with your cable company, their advertisers and your

ress release concluded with the message that this is · a matter of awareness , and having a choice iri

atter.

minimum, consumers should have the option of saying no to being watched. " d it interesting . At first I thought it was another over reach of govenunent then I looked at the

Dire TV DVR box and re-read the article.

I realized this wasn't written specifically for the Xbox One.

This was written and aimed at Cable companies with DVR's. I had already read the comments section

at

s point and most of the comments were along the lines of. . . . er solution, don't buy an,

an Xbox. There i s n o need for a law. "

this is so over the top"

I.

I

Then

e post I replied to . . .

"Micr soft and other c omp anie s will make it al-o r-none . Either you enable the earnerďż˝ to transmit back

to the orne office, or you don't use the camera If

t

for anything. I still don't see the need for a federal law.

don't want to be survei lled, don't buy an Xbox. Sony or Nintendo will come out with a

co mp titive product that is not so intrusive. Fre My I too

this until

l

market

win."

I looked at my cable box. What if cable companies decided to do thisl We're

very I mited in what cable companies you can use. Without any oversight and very little effort, the

cable �ompanies could work together and push that out. Most people will do without a gaming

conso�es, how many would actually say no to cable?

I

f

Some ne else rep lyi ng to me

You :must admit tho ugh, the princ iple is the exact same no matter how much you want the product.

1/ I

I

I

I

My Reply back

While I do agree the federal government should be very limited in its interference with

anything, this is not one of those cases. The government has a j ob of protecting your right to

privacy. It is one of the few things the government is supposed to do. I have no prob lem with

the government telling co rporations that they us.

can not punish us if we don't allow them to spy o n

The same guy replying back to n1e

The government has no such duty to protect your right of privacy in voluntary transactions I between private citizens/entities. You have no more right to an xbox without a camera than a I right to vegetables without genetically modified organis ms . Nor do yo u have a ri ght to cable.

And what if you did? Take it to the logical conclusion, what other freely produced/purchased items would you then have the right to force (by the State, legislature) producers to make

according to your whim? I don't like being spied on, so I won't buy the pro duct . That's where

the analysis stops. My reply back

It does when it is the one using said corporations to spy on the citizens. Cable companies are

also not entirely private entities. They are highly subsidized by big government. If they were

truly priv ate entities many non..; spying

ones would pop up as soon all the maj or ones started spying. Ev ery home would have a choice of 2 or more cable providers but that isn't the case for

most people.

I don't eat GMO's but would agree the government has no right to make companies label GMO products .

Fre e Market works just fine because non-GMO foods make sure it is written al over.

As a co nsumer you're left with many choices.

Cabl e companies are comp letely different. It's about the control of information. If cable

companies tomorrow could tell you that you either put in a camera or they stop pro v iding you

TV/Internet access, you're at a serious disadvantage in life. �th the way things are going, that isn't completely far-fetched.

In a truly free society we wouldn't need this, but that's not where we are in history.

His Final Reply back to me. exists through State action. So maybe it is a different analysis with cable. But that is one of a

very few industries where that is the case. (Thankfully) It

as an interesting back and forth. I think I would actually argue that even in a case like the Xbox

One, as a consumer you should have the option of buying that hardware without the spy cam on it. I thi at some point, a conversation has to take place about the true role of the constitution and the fed ral government. I'm one of those that believes the federal governments j ob is to protect the bill of Sometimes the few need to be protected from the masses when it comes to the bill of rights. If rig 8 0° o of the population decided tomorrow that they don't care about their right to privacy and every

co pany out there started to put a c amera, microphone, and internet connection on their devices then req ·red you to have those parts operational in order to use the device, what happens to the 20% of pe le that don't want their privacy stolen from them? I'm talking about a stove, a microwave, a

· gerator, your cable box, your AC unit. This seems far fetched for now, but 20-3 0 years from now, I

do 't know that it will be as far fetched. I completely understand that in a truly free republic, this

wo ld never happen. But that's not what we have. We have a society filled with idiots that don't care

ab t any of their rights and will give them up for the latest gadget and a government that is loving the r new abilities to spy on the people. I think people need to be cautious when it comes to the kind of

}

leg slation. As I pointed out earlier in my replies, I don't think foods that contain GMO's should have to e labeled. I'm more concerned about a person's right to privacy in their home.

Fe eral Nullification efforts mounting in states . . . . Im gine the scenario: A federal agent attempts to arrest someone for illegally selling a machine gun. Ins ead, the federal agent is arested - charged in a state court with the crime of enforcing federal gun la s . Farfetched? Not as much as you might think.

Th scenario would become conceivable if legislation passed by Missouri's Republican-led Legislature � is s gned into law by Democratic Gov. Jay Nixon. be

Missouri legislation is perhaps the most extreme example of a states ' rights movement that has spreading across the nation. States are increasingly adopting laws that purport to nullify federal

s -. setting up intentional legal conflicts, directing local police not to enforce federal laws and, in rar cases, even threatening criminal charges for federat agents who dare to do their j obs. An Associated Press analysis found that about four-fifths of the states now have enacted local laws that

dir ctly rej ect or ignore federal laws on m arij uana use, gun control, health insurance requirements and

standards for driver 's licenses. The trend began in Democratic leaning California with a 1 9 6 medical marijuana law and has proliferated lately in Republican strongholds like Kansas, where Go . Sam Brownback this spring became the frrst to sign a measure threatening felony charges against ide

fed ral agents who enforce certain firearms laws in his state. So

e states, such as Montana and Arizona, have said "no" to the feds again and again

es' rights measures on all four subjects examined by the AP

"n " caries any legal significance.

-

-

passing

despite questions about whether their

"It see ¡s that there has been an uptick in nullification efforts from both the left and the right," said a professor at the University of California at Los Angeles who

Adam consti

Yet '

law.

specializes in

e law is clear - the supremacy clause (of the U.S. C onstitution) says specifically that the

laws are supreme over contrary state laws, even if the state doesn't like those laws," Wm.k.ler

feder

added

over some tates from flouting those federal laws - sometimes successfully.

state ones hasn't stopped

20 states now have medical marijuana laws allowing people to use pot to treat chronic pain and

other 'lments - despite a federal law that still criminalizes marijuana distribution and possession.

C edin ground to the states, President B arack Obama's administration has mad e it known to federal

prose utors that it wasn't worth their time to target those people.

authorities have repe atedly delayed implementation of the 2005 Real ID Act,

law or

r

an

anti-terrorism

set stringent requirements for photo identification cards to be used to board commercial flights

federal buildings. The law has been stymied, in part, because about half the state legislatures

have pposed its implementation, according to the National Conference of State Legislatures .

enacted measures challenging Obama's 20 1 0 health care laws, many of which sp e ci tcally rej ect the provision mandating that most people have health insurance or face tax penaltie s b e gi ing in 20 1 4 . Abou 20 states have

After Montana passed a

2009 law decl aring that federal fireans regulations don't apply to guns made

pt in that state, e i ght other states have enacted similar laws.

and The feder

upreme Court ruled in

1 997 that local police could not be compelled to carry out provisions of a

gun control law. But some states are now attempting to take that a step further by asserting that

certa n fede ral laws can't even be enforced by federal authorities .

Kans as law makes it a felony fo r a federal agent to attempt to enforce l aws on guns made and own d in Kansas. A similar Wyoming law, passed in 20 1 0, made it a misdeme anor. The Missouri bill

A ne

ould declare it a misdemeanor crime but would apply more broadly to all federal gun laws and reg ations - past, present, or future - that "infringe on the people's ri ght to keep and bear arms."

also

U. S .

General Eric Ho lder

sent a letter in late April to the Kansas governor warning that the al government is willing to go to court over the nevv lav.'. " Kansas may not prevent federal

emp oyees and officials from caring out their official responsibilities," Holder wrote. So "

woulďż˝ happen if a local pro sec utor actually charges a federal agent for doing his or her job?

Th y're going to have problems if they do it - there's no doubt about it," said Michael Boldin, tive director of the Tenth Amendment Center, a Los Angeles-based entity that promotes states'

exe

righ s . "There ' s no federal court in the country that's going to say that a state can pull this off. "

Yet tates may never need t o prosecute federal agents i n order t o make their point. If eno ug h states resi , "it's going to be very difficult for the federal government to force their l aws down our throats,"

Bol in said.

bill nullifying federal gun laws. whi le , thousands of people have sent online messages to the governor 's office about the legi lation.

Mis ouri 's governor has not said whether he will sign or veto the

Me

Signi g the measure "will show other states how to resist the tyrany of federal bureaucrats who want to ro you of your right to self-defense," said one message, si gned by Tim and Arlena Sowash, who gun shop in rural Stover, Mo. 路

e? dang time! People need to realize that the federal government does not have our best interest in If we can't take our country back with elections, maybe we can take parts back with nullification


Michael Dunn Letters From Jail