Columns for Special Applications
Separation of Licorice Root on RRHD Columns Column A:
Column B: Column C: Mobile Phase:
Flow Rate: Gradient: Temperature: Detector: Instrument:
ZORBAX RRHD SB-C18 857700-902 2.1 x 50 mm, 1.8 μm 858700-902 2.1 x 100 mm, 1.8 μm 859700-902 2.1 x 150 mm, 1.8 μm 10-100% B/30 min A: 0.1% formic acid (fa) B: acetonitrile with 0.1% fa F = 0.4 mL/min 30 minute gradient on each length Ambient 280 nm UV 1290 Infinity LC
50 mm
7 peaks
A
Rs: 0
mAU 200 150 100 50 0
2
0
2
4
6
8
10
12
4
14
16
18
min
100 mm
8 peaks
B
Rs: 1.37
mAU 200 150 100 50 0
2
0
2
4
6
8
10
12
4
14
16
18
min
150 mm
9 peaks
C
Rs: 2.40
mAU 200 150 100 50 0
2
0
2
4
6
8
10
12
4
14
6
16
18
min RRHD_Licorice
LC AND LC/MS COLUMNS
893