Columns for Analytical HPLC
Optimize Separations with Eclipse XDB Selectivity Options: Analysis of Sunscreens Column A:
Column B:
Column C:
Eclipse XDB-Phenyl 963967-912 4.6 x 150 mm, 3.5 μm Eclipse XDB-C8 963967-906 4.6 x 150 mm, 3.5 μm Eclipse XDB-C18 963967-902 4.6 x 150 mm, 3.5 μm
A
1
1. Oxybenzone 2. Padimate-O 3. Ethylhexylsalicylate
2 3
Mobile Phase: Flow Rate: Temperature: Sample:
0 B
15% H2O:85% MeOH 1.0 mL/min 35°C Sunscreens
2
4
6
8
10
12
14
16
10
12
14
16
1
2 3
0 C
This separation of sunscreens on all three Eclipse XDB bonded phases – C18, C8 and Phenyl – shows that different bonded phases can be used to optimize a separation. While all three bonded phases provide an adequate separation, the Eclipse XDB-Phenyl provides a different peak elution order and a much shorter overall analysis time. All three bonded phases also provide excellent peak shape with no mobile phase additives.
2
4
6
8
1
2 3
0
2
4
6
8 10 Time (min)
12
14
LCEC007
Selectivity for Urea Pesticides Column A:
Column B:
Column C:
Mobile Phase:
Flow Rate: Temperature: Sample:
Eclipse XDB-C18 993967-902 4.6 x 150 mm, 5 μm Eclipse XDB-CN 993967-905 4.6 x 150 mm, 5 μm Eclipse XDB-C18 993967-902 4.6 x 150 mm, 5 μm A. 60:40 MeOH:Water B. 60:40 MeOH:Water C. 77:23 MeOH:Water 1.0 mL/min 25°C Urea pesticides
A
2 3
4
1
5 6
0
10
B
30 Time (min)
40
50
2 34
5
1
0
2
C
2
6
4 6 Time (min) 34
2
8
10
8
10
5
1
0
20
1. 2. 3. 4. 5. 6.
Fenuron Monuron MonoLinuron Diuron Linuron Pencycuron
6
4 6 Time (min)
The Eclipse XDB-CN column reduces retention time and provides good selectivity for Urea pesticides when compared to an Eclipse XDB-C18 column.
834
LC AND LC/MS
www.agilent.com/chem/lccolumns
16
LCEC010