Gene Therapy & Molecular Biology Volume 2 Issue A

EMBED Excel.Chart.5 \s Figure 3. Post rV-CEA vaccinated PBL, stimulated in vitro with CAP-1 peptide display an oligoclonal 6 expansion of T cell receptor V family subtypes. Total cellular RNA was isolated from 5 x 10 V24 T cells. Firststrand cDNA was then synthesized from 1 g of total RNA and ampliďƒžed with 25 V oligonucleotides and FITClabeled C oligonucleotide. Labeled PCR products were loaded on a 6% acrylamide sequencing gel and the samples were then run on an ABI 373 sequencer for size and ďƒ&#x;ourescence intensity determination. The relative percentages of each V subfamily are represented as histograms.